Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma

Abstract

Chaperonin containing TCP1 subunit 7 (CCT7) regulates the expression of many tumor-related proteins. We investigated the diagnostic and prognostic value of CCT7 expression for hepatocellular carcinoma (HCC). In datasets from The Cancer Genome Atlas and the Gene Expression Omnibus, CCT7 mRNA levels were greater in HCC tissues than adjacent normal tissues, and these results were validated using immunohistochemistry. In patients with early-stage disease and low alpha-fetoprotein expression, CCT7 expression was still higher in HCC tissues than normal tissues. Receiver operating characteristic curve analyses indicated that CCT7 expression had better diagnostic value than alpha-fetoprotein for HCC patients with early-stage disease and low alphafetoprotein expression. The positive predictive value of CCT7 expression was higher than that of alphafetoprotein expression. Higher CCT7 mRNA and protein levels were independent risk factors for poorer overall and recurrence-free survival in HCC patients. Greater methylation of the CpG site cg19515186 was associated with better overall survival in HCC patients. Genes co-expressed with CCT7 were upregulated in HCC and associated with poorer overall survival. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and Gene Set Enrichment Analyses demonstrated that CCT7 expression correlated with spliceosome signaling. These findings demonstrate that CCT7 has diagnostic and prognostic value for HCC.

Prognostic related gene index for predicting survival and immunotherapeutic effect of hepatocellular carcinoma

“…CCT7 has diagnostic and prognostic value for HCC. [ 57 ] High PRC1 expression in HCC is associated with poor prognosis and low macrophage infiltration. [ 58 ] This finding is consistent with the results of the present analysis.…”

Elevated Small Nuclear Ribonucleoprotein Polypeptide an Expression Correlated With Poor Prognosis and Immune Infiltrates in Patients With Hepatocellular Carcinoma

“…In addition, KEGG and GSEA analysis revealed that SNRPA was involved in the spliceosome signaling pathway. Interestingly, numerous studies have shown that spliceosome signaling contributed enormously to the tumorigenesis and advancement of multiple tumor types ( 27 , 40 , 41 ). More importantly, in our previous study, the genes most relevant to survival were mainly enriched in the spliceosome pathway in the GEPIA database ( 40 ).…”

“…Interestingly, numerous studies have shown that spliceosome signaling contributed enormously to the tumorigenesis and advancement of multiple tumor types ( 27 , 40 , 41 ). More importantly, in our previous study, the genes most relevant to survival were mainly enriched in the spliceosome pathway in the GEPIA database ( 40 ). Based on the above research results, it is reasonable to speculate that SNRPA functions as an oncogene that promotes HCC tumorigenesis and progression via the spliceosome signaling pathway, which needs further study.…”

TMEM88 is a protective gene in HCC and has the function of promotes immune escape

“…3,4 The low survival rate of patients with HCC is due to the low detection rate in early HCC and early recurrence post-curative resection. 5 Although diagnostic and prognostic biomarkers have been identi ed in recent years, their poor sensitivity and speci city limit clinical application. 6,7 Therefore, new diagnostic and prognostic molecular biomarkers and the relevant molecular mechanisms and signaling pathways urgently need to be identi ed to improve the prognosis of patients with HCC.…”