RETRACTED ARTICLE: Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc

Abstract

Retinoblastoma is a rare cancer of the immature retina. This study designed to see the function of the lncRNA ANRIL in retinoblastoma Y79 cells. ANRIL, miR-99a and c-Myc expression in Y79 cells was altered by transfection and then trypan blue, transwell assay and flow cytometry were carried out to evaluate the changes of cell phenotype. The connection between ANRIL, miR-99a and c-Myc was measured by luciferase reporter assay and RNA immunoprecipitation analysis. As a result, ANRIL expression was highly expressed in human retinoblastoma tissue as relative to the adjacent noncancerous tissues. ANRIL suppression inhibited Y79 cells viability, migration, invasion, while promoted apoptosis. ANRIL negatively regulated miR-99a by binding to miR-99a. Silence of miR-99a reversed the ANRIL-knockdown effects on Y79 cells. miR-99a overexpression suppressed Y79 cell viability, migration, invasion, and enhanced apoptosis through downregulating c-Myc. Meanwhile, we found that miR-99a inhibited JAK/ STAT and PI3K/AKT pathways. To conclude, it seems that ANRIL suppression inhibits cell growth and metastasis in retinoblastoma Y79 cells by regulating miR-99a and c-Myc.

Mbd2 Mediates Retinal Cell Apoptosis by Targeting the lncRNA Mbd2-AL1/miR-188-3p/Traf3 Axis in Ischemia/Reperfusion Injury

“…[32][33][34] For example, lncRNA antisense noncoding RNA in the INK4 locus (ANRIL) serves as a competing endogenous RNA to sponge miR-99a and promote apoptosis in retinoblastoma Y79 cells. 35 Hence, we predicted that miR-188-3p was a target gene of lncRNA Mbd2-AL1. Several experiments were performed to demonstrate this hypothesis.…”

Long Noncoding RNA TRPM2-AS Promotes the Growth, Migration, and Invasion of Retinoblastoma via miR-497/WEE1 Axis

“…The lncRNA TP73-AS1 expression was upregulated in RB tissues and cells and it had oncogenic functions in RB (Wang et al, 2020). Antisense noncoding RNA in the INK4 locus (ANRIL) was also elevated and acted as onco-lncRNA in RB (Wang et al, 2019b;Yu et al, 2019).…”

Retracted Article: Long noncoding RNA ANRIL knockdown increases sensitivity of non-small cell lung cancer to cisplatin by regulating the miR-656-3p/SOX4 axis

“…5,6 LncRNA antisense noncoding RNA in the INK4 locus (ANRIL), also known as cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1), has been reported to act as an oncogene to promote the development of multiple cancers. [7][8][9] More importantly, previous studies suggest that ANRIL could encourage the development of drug resistance in gastric cancer, osteosarcoma and ovarian cancer. [10][11][12] Although the carcinogenesis of ANRIL has been exhibited in NSCLC development, 13,14 whether this lncRNA could regulate CDDP resistance in NSCLC remains unclear.…”