RETRACTED ARTICLE: miR-376a inhibits the proliferation and invasion of osteosarcoma by targeting FBXO11

Xu, Qiaolong; Cheng, Li; Chen, Jianyang; Lu, Wenjie; Wang, Peinian

doi:10.1007/s13577-019-00256-2

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…Downregulation of miR-127 has been shown to be a common feature in several types of cancer, including ovarian cancer [27], gastric cancer [28], squamous cell carcinoma [26], pancreatic cancer [31], giant cell tumor of bone [35], and osteosarcoma [36]. Likewise, downregulation of miR-376a has been reported in multiple cancer types, including breast cancer [32], lymphoma [30], non-small-cell lung cancer [29], giant cell tumor of bone [35], and osteosarcoma [37]. In these studies, both miRNAs have been shown to inhibit proliferation, migration, and invasion of cancer cells and to induce apoptosis upon restoration.…”

Section: Discussionmentioning

confidence: 99%

“…Since miRNAs represent highly attractive targets for the development of new therapeutic strategies, and tumor suppressor functions of miR-127 and miR-376a have already been demonstrated in osteosarcoma cell lines in vitro [36][37][38], we aimed to validate these tumor suppressor functions in further osteosarcoma cell lines and further to analyze the impact of candidate miRNA restoration on tumor engraftment and growth in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Tumor Suppressor Function of miR-127-3p and miR-376a-3p in Osteosarcoma Cells

Fellenberg

Lehner

Saehr

et al. 2019

Cancers

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Since the introduction of high-dose chemotherapy about 35 years ago, survival rates of osteosarcoma patients have not been significantly improved. New therapeutic strategies replacing or complementing conventional chemotherapy are therefore urgently required. MicroRNAs represent promising targets for such new therapies, as they are involved in the pathology of multiple types of cancer, and aberrant expression of several miRNAs has already been shown in osteosarcoma. In this study, we identified silencing of miR-127-3p and miR-376a-3p in osteosarcoma cell lines and tissues and investigated their role as potential tumor suppressors in vitro and in vivo. Transfection of osteosarcoma cells (n = 6) with miR-127-3p and miR-376a-3p mimics significantly inhibited proliferation and reduced the colony formation capacity of these cells. In contrast, we could not detect any influence of miRNA restoration on cell cycle and apoptosis induction. The effects of candidate miRNA restoration on tumor engraftment and growth in vivo were analyzed using a chicken chorioallantoic membrane (CAM) assay. Cells transfected with mir-127-3p and miR-376a-3p showed reduced tumor take rates and tumor volumes and a significant decrease of the cumulative tumor volumes to 41% and 54% compared to wildtype cells. The observed tumor suppressor function of both analyzed miRNAs indicates these miRNAs as potentially valuable targets for the development of new therapeutic strategies for the treatment of osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tumor Suppressor Function of miR-127-3p and miR-376a-3p in Osteosarcoma Cells

Fellenberg

Lehner

Saehr

et al. 2019

Cancers

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“…MiR-376a is frequently reported to be upregulated in ovarian cancer [36] and downregulated in renal cell carcinoma [37], gastric cancer [38], and breast cancer [39]. MiR-376a is underexpressed in OS tumors and cell lines [40]. Its upregulation inhibits OS cell proliferation and invasion in vitro but promotes apoptosis [40].…”

Section: Discussionmentioning

confidence: 99%

“…MiR-376a is underexpressed in OS tumors and cell lines [40]. Its upregulation inhibits OS cell proliferation and invasion in vitro but promotes apoptosis [40]. In terms of the mechanism, F-box protein 11 mRNA is proved to be a direct target of miR-376a in OS [40].…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA TTN-AS1 enhances the malignant characteristics of osteosarcoma by acting as a competing endogenous RNA on microRNA-376a thereby upregulating dickkopf-1

Liu

Pei

et al. 2019

Aging

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The expression levels and detailed functions of TTN-AS1 in osteosarcoma (OS) have not yet been explored. This study aimed to measure TTN-AS1 expression in OS tissues and cell lines, investigate its specific roles in the aggressive characteristics of OS cells in vitro and in vivo, and elucidate the regulatory mechanisms of TTN-AS1 action. TTN-AS1 expression was high in OS tissue samples and cell lines; TTN-AS1 overexpression correlated with the clinical stage, distant metastasis, and shorter overall survival of the patients. A TTN-AS1 knockdown inhibited OS cell proliferation, migration, and invasion and induced apoptosis in vitro and slowed tumor growth in vivo. Mechanism investigation revealed that TTN-AS1 acts as a competing endogenous RNA on microRNA-376a-3p (miR-376a) in OS cells. Dickkopf-1 (DKK1) mRNA was identified as a direct target of miR-376a in OS cells. Resumption of DKK1 expression reversed the tumor-suppressive activities of miR-376a overexpression in OS cells. The knockdown of miR-376a counteracted the reduction in the malignant characteristics of OS cells by the downregulation of TTN-AS1. In conclusion, TTN-AS1 functions as a competing endogenous RNA targeting miR-376a and increases the malignancy of OS cells in vitro and in vivo by upregulating DKK1.

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“…This article [1] has been retracted by the editors because the data are unreliable. It appears that the images in Fig.…”

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confidence: 99%

Retraction Note to: miR-376a inhibits the proliferation and invasion of osteosarcoma by targeting FBXO11

Xu¹,

Cheng²,

Jian-yang³

et al. 2020

Human Cell

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RETRACTED ARTICLE: miR-376a inhibits the proliferation and invasion of osteosarcoma by targeting FBXO11

Cited by 12 publications

References 15 publications

Tumor Suppressor Function of miR-127-3p and miR-376a-3p in Osteosarcoma Cells

Tumor Suppressor Function of miR-127-3p and miR-376a-3p in Osteosarcoma Cells

Long noncoding RNA TTN-AS1 enhances the malignant characteristics of osteosarcoma by acting as a competing endogenous RNA on microRNA-376a thereby upregulating dickkopf-1

Retraction Note to: miR-376a inhibits the proliferation and invasion of osteosarcoma by targeting FBXO11

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