RETRACTED ARTICLE: Degradable poly-L-lysine-modified PLGA cell microcarriers with excellent antibacterial and osteogenic activity

Abstract

The surface modification of polymeric materials has become critical for improving the bone repair capability of materials. In this study, we used a poly-L-lysine (PLL) coating method to prepare functional poly (lactic acid-glycolic acid) (PLGA) cell microcarriers, and bone morphogenetic protein 7 (BMP-7) and ponericin G1 were immobilized on the surface of microcarriers. The scanning electron microscopy (SEM), water contact angle measurement, and energy-dispersive Xray spectroscopy (EDX) was used to analyse the surface morphology of PLL-modified PLGA microcarriers (PLL@PLGA) and their ability to promote mineralization. At the same time, the growth factor binding efficiency and antimicrobial activity of the microcarriers were studied. The effects of microcarriers on cell behaviors were evaluated by cultivating MC3T3-E1 cells on different microcarriers. The results showed that the hydrophilicity, protein adsorption, and mineralization induction capability of the microcarriers were significantly improved by PLL surface modification. The biological experiments revealed that BMP-7 and ponericin G1 immobilized-PLL modified microcarriers can effectively inhibit the proliferation of pathogenic microorganisms while enhancing the ability of the microcarriers to promote cell proliferation and osteogenesis differentiation. Therefore, we believe that PLL-modified PLGA cell microcarriers loaded with BMP-7 and ponericin G1 (PLL@PLGA/BMP-7/ponericin G1) have great potential in the field of bone repair.

Investigation of the structure‐activity relationship in ponericin L1 from Neoponera goeldii

“…The ponericin G and W families both contain a conserved Pro residue that lies near the middle of the sequence in 11 of 13 sequences (the other two sequences, G6 and G7, the Pro is near the C‐terminus due to the shorter overall length). A number of groups have investigated the peptides from the G‐ and W‐families of ponericins, [ 28‐33 ] and other groups have investigated novel peptides that show similarity to the G‐ and W‐families. [ 34‐37 ] A comparison of ponericin sequences are shown in Supplemental Table S1.…”

Advanced Surface Modification for 3D-Printed Titanium Alloy Implant Interface Functionalization

“…Polylactide glycolic acid (PLGA) is one of the most widely used degradable materials for medical applications. PLGA has excellent mechanical properties, low immunogenicity, low toxicity, and adjustable degradation rate ( Kong J. et al, 2018 ; Zhang H et al, 2019 ). Diabetes is a high-risk factor leading to implant failure.…”

Nanobiotechnology with Therapeutically Relevant Macromolecules from Animal Venoms: Venoms, Toxins, and Antimicrobial Peptides

“…This scaffold material has appropriate morphological and surface properties that can meet the needs of skin tissue regeneration [ 44 ]. Ponericin-G1 encapsulated in PLGA/PLL also showed effectiveness against Staphylococcus aureus and E. coli at 300 μg mL −1 ; PLL surface modification and ponericin G1 can greatly improve the antimicrobial activity of the cell microcarrier and enhance the clinical application value of microcarriers [ 132 ].…”