RETRACTED ARTICLE: Bilobalide protects H9c2 cell from oxygen-glucose-deprivation-caused damage via upregulation of miR-27a

Cao, Axiu; Li, Xiangting

doi:10.1080/21691401.2019.1640708

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…Hua et al ( 51 ) revealed that BB could protect neural cells against aggregated α-synuclein-induced apoptosis. Cao and Li ( 52 ) observed that BB ameliorated OGD-induced injury in H9c2 cells. A study by Mao et al ( 53 ) demonstrated that BB alleviated IL-17-induced inflammatory injury in ATDC5 cells.…”

Section: Discussionmentioning

confidence: 99%

Bilobalide attenuates lipopolysaccharide‑induced HepG2 cell injury by inhibiting TLR4‑NF‑κB signaling via the PI3K/Akt pathway

Mao,

Yao,

Zhang

et al. 2023

Exp Ther Med

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Inflammation is involved in the pathological process underlying a number of liver diseases. Bilobalide (BB) is a natural compound from Ginkgo biloba leaves that was recently demonstrated to exert hepatoprotective effects by inhibiting oxidative stress in the liver cancer cell line HepG2. The anti-inflammatory activity of BB has been reported in recent studies. The major objective of the present study was to investigate whether BB could attenuate inflammation-associated cell damage. HepG2 cells were cultured with lipopolysaccharide (LPS) and BB, and cell damage was evaluated by measuring cell viability using MTT assay. The activity of the NF-κB signaling pathway was assessed by measuring the levels of IκBα, NF-κB p65, phosphorylated (p)-IκBα, p-p65, p65 DNA-binding activity and inflammatory cytokines IL-1β, IL-6 and TNF-α. A toll-like receptor (TLR)4 inhibitor (CLI-095) was used to detect the involvement of TLR4 in cell injury caused by LPS. In addition, the PI3K/Akt inhibitor LY294002 was applied to explore the involvement of the PI3K/Akt axis in mediating the effects of BB. The results demonstrated that LPS induced HepG2 cell injury. LPS also elevated the levels of p-IκBα, p-p65, p65 DNA-binding activity and inflammatory cytokines. However, CLI-095 significantly attenuated the LPS-induced cell damage and inhibited the activation of NF-κB signaling. BB also dose-dependently attenuated the LPS-induced cell damage, activation of NF-κB signaling and TLR4 overexpression. Furthermore, it was observed that LY294002 diminished the cytoprotective effects of BB on cell injury, TLR4 expression and NF-κB activation. These findings indicated that BB could attenuate LPS-induced inflammatory injury to HepG2 cells by regulating TLR4-NF-κB signaling.

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Section: Discussionmentioning

confidence: 99%

Bilobalide attenuates lipopolysaccharide‑induced HepG2 cell injury by inhibiting TLR4‑NF‑κB signaling via the PI3K/Akt pathway

Mao,

Yao,

Zhang

et al. 2023

Exp Ther Med

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“…Post-transcriptional or post-translational regulation, such as 3′UTR modification, acetylation, or methylation may be involved; miR-449a-5p [ 43 ], miR-34a-5p [ 44 ], and miR-30b-5p [ 45 ] have been found to be responsible for SIRT1-targeted inhibition. BB regulates the expression of miRNAs such as miR-101-5p and miR-27a-5p, which have multiple biological functions [ 46 ]. Synthetically, Both AMPK and SIRT1 are energy-sensing molecules that have coexisted in cells throughout evolution.…”

Section: Discussionmentioning

confidence: 99%

Gingko biloba-inspired lactone prevents osteoarthritis by activating the AMPK-SIRT1 signaling pathway

Zhao

Liu

et al. 2022

Arthritis Res Ther

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Background Uncoupled extracellular matrix (ECM) causes cartilage degeneration and osteoarthritis (OA) by suppressing the synthesis and activating the degradation of ECM components. Gingko biloba is a natural Chinese herb with a variety of biological functions; however, the extent to which it can protect against OA and the mechanisms involved are unknown. Methods In our study, using bioinformatics tools, we were able to identify an important lactone, bilobalide (BB), from Gingko biloba. In vitro experiments were performed to evaluate the potential therapeutic effects of BB on ECM homeostasis. In vivo experiments were conducted to assess the protection of systemic administration of BB on cartilage degeneration. Molecular mechanisms underlying BB-regulated anti-arthritic role were further explored. Results In interleukin-1β-incubated human chondrocytes, in vitro treatment with BB increased the expression of cartilage anabolic proteins, while inhibiting the activities of ECM degrading enzymes. In a mice model, systemic administration of BB, in vivo, prevented post-traumatic cartilage erosion and attenuated the formation of abnormal osteophytes in the subchondral bone. Mechanistically, the activation of the adenosine 5′-monophosphate-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) signaling pathway was involved in the anti-arthritic effects of BB. In vitro, blocking BB’s chondroprotection with the AMPK-specific inhibitor Compound C abrogated it. Conclusions These results demonstrated that BB extracted from Gingko biloba regulates ECM balance to prevent OA by activating the AMPK-SIRT1 signaling pathway. This study proposed the monomer BB, a traditional Chinese medicine, as a de novo therapeutic insight for OA. Graphical Abstract Schematic representation of the experimental design. Based on the bioinformatic analysis, bilobalide (BB), a natural herb Gingko biloba-derived ingredient, was identified as a candidate for treating osteoarthritis. In vitro, BB treatment not only facilitates cartilage extracellular matrix synthesis but also inhibits proteolytic enzyme activities. In vivo intraperitoneal injection of BB improves cartilage degeneration and subchondral bone sclerosis. BB, in particular, had anti-arthritic effects by activating the AMPK-SIRT1 signaling pathway.

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“…MI may cause perioperative ischemia and infarction (Pagliaro, Cannata, Stefanini, & Bolognese, 2020; Smit, Coetzee, & Lochner, 2020). Cao and Li (2019) established myocardial ischemic injury employing oxygen–glucose deprivation (OGD) and studied the role and mechanism of BB in OGD‐induced damage to rat myocardial H9c2 cells. It was found that BB can upregulate miR‐27a, activate PI3K/Akt and Wnt/β‐catenin signaling pathways, reduce the damage of H9c2 cells caused by OGD, improve cell viability, and reduce cell apoptosis to relieve MI.…”

Section: Pharmacological Effects Of Bbmentioning

confidence: 99%

Bilobalide: A review of its pharmacology, pharmacokinetics, toxicity, and safety

Xie

et al. 2021

Phytotherapy Research

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Bilobalide is a natural sesquiterpene trilactone from Ginkgo biloba leaves. It has good water solubility and is widely used in food and pharmaceutical fields. In the last decade, a plethora of studies on the pharmacological activities of bilobalide has been conducted and demonstrated that bilobalide possessed an extensive range of pharmacological activities such as neuroprotective, antioxidative, antiinflammatory, antiischemic, and cardiovascular protective activities. Pharmacokinetic studies indicated that bilobalide may have the characteristics of rapid absorption, good bioavailability, wide distribution, and slow elimination. This review aims to summarize the advances in pharmacological, pharmacokinetics, toxicity, and safety studies of bilobalide in the last decade with an emphasis on its neuroprotective and antiinflammatory activities, to provide researchers with the latest information and point out the limitations of relevant research at the current stage and the aspects that should be strengthened in future research.

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RETRACTED ARTICLE: Bilobalide protects H9c2 cell from oxygen-glucose-deprivation-caused damage via upregulation of miR-27a

Cited by 8 publications

References 48 publications

Bilobalide attenuates lipopolysaccharide‑induced HepG2 cell injury by inhibiting TLR4‑NF‑κB signaling via the PI3K/Akt pathway

Bilobalide attenuates lipopolysaccharide‑induced HepG2 cell injury by inhibiting TLR4‑NF‑κB signaling via the PI3K/Akt pathway

Gingko biloba-inspired lactone prevents osteoarthritis by activating the AMPK-SIRT1 signaling pathway

Bilobalide: A review of its pharmacology, pharmacokinetics, toxicity, and safety

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