Retinoprotective effect of agmatine in streptozotocin-induced diabetic rat model: avenues for vascular and neuronal protection

Gheit, Rehab E. Abo El; Soliman, Nema A.; Badawi, Ghada A.; Madi, Nermin M.; El-Saka, Mervat H; Badr, Shimaa M.; Emam, Marwa Nagy

doi:10.1007/s13105-021-00799-9

Cited by 14 publications

(6 citation statements)

References 37 publications

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“…Counterstaining by haematoxylin was performed (Buchwalow & Böcker, 2010). Under light microscope, immunostaining was scored using a digital imaging software program "ImageJ" (ImageJ, National Institutes of Health, Bethesda, USA, RRID:SCR_003070) at a magnification of 400 (Abo El Gheit et al, 2021). The experimental details provided for micrograph acquisition and analysis comply with the recommendations made by the British Journal of Pharmacology Guidelines (Alexander et al, 2018).…”

Section: Light Microscopic Examinationmentioning

confidence: 99%

Melatonin epigenetic potential on testicular functions and fertility profile in varicocele rat model is mediated by silent information regulator 1

Gheit

Soliman

Nagla

et al. 2022

British J Pharmacology

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Background and Purpose Varicocele is a leading cause of male infertility. Melatonin is a highly pleiotropic neurohormone. We aimed to characterize the melatonin epigenetic potential in varicocele and the involved molecular mechanisms. Experimental Approach Fifty‐two male albino rats were randomly divided into four groups (13 rats each): control (I), melatonin (II), varicocele (III) and melatonin treated varicocele (IV) groups. Left varicocele was induced by partial left renal vein ligation. Reproductive hormones, epididymal sperm functional parameters, testicular 3/17 β‐hydroxysteroid dehydrogenases, antioxidant enzymes, malondialdehyde, nicotinamide adenine dinucleotide phosphate oxidase, 8‐hydroxy‐2′‐deoxyguanosine and histopathological/Johnsen's score were evaluated. Flow cytometry and Comet were carried out to explore extent of sperm and testicular DNA damage. Testicular expression of silent information regulator 1 (SIRT1), forkhead transcription factors‐class O (type1) (FOXO1), tumour suppressor gene, P53, cation channels of sperm (CatSper) and steroidogenic acute regulatory protein was evaluated by western blot technique. Testicular expression of Bcl‐2 and its associated X protein and nuclear factor kappa‐light‐chain‐enhancer of activated B cells were assayed by immunohistochemical staining. Testicular miR‐34a expression was quantified by quantitative reverse transcription‐polymerase chain reaction. Key Results The varicocele induced testicular histological injury, enhanced oxidative stress, P53‐mediated apoptosis, DNA damage and increased testicular miR‐34a expression paralleled with down‐regulated SIRT1/FOXO axis. Melatonin treatment of varicocele rats displayed antioxidant/anti‐apoptotic efficacy and improved reproductive hormones axis, CatSper expression and fertility parameters. MiR‐34a/SIRT1/FOXO1 epigenetic axis integrates testicular melatonin mediated intracellular transduction cascades in varicocele. Conclusion and Implications Melatonin can be used as an adjuvant therapy to improve varicocele and its complication.

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Section: Light Microscopic Examinationmentioning

confidence: 99%

Melatonin epigenetic potential on testicular functions and fertility profile in varicocele rat model is mediated by silent information regulator 1

Gheit

Soliman

Nagla

et al. 2022

British J Pharmacology

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“…GLP-1 receptor agonists (GLP-1Ras) and dipeptidyl peptidase-4 inhibitors (DPP-4Is) are commonly used for the treatment of T2DM [ 27 ]. Since endocitotoxicity has more recently been identified as a key pathological mechanism underlying DR [ 28 – 31 ], systemic neuroprotective therapies for extraocular disease are likely to indirectly provide benefits for ocular disease, although this has yet to be shown [ 32 , 33 ]. Based on this robust pathophysiological background with respect to the role of Reg proteins in excitotoxicity and neuroprotection in systemic disease, we were surprised that their potential role in the diabetic retina has not previously been reported.…”

Section: Discussionmentioning

confidence: 99%

Association of inflammation-related markers and diabetic retinopathy severity in the aqueous humor, but not serum of type 2 diabetic patients

Saucedo,

Pfister,

Schild

et al. 2023

PLoS ONE

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Diabetic retinopathy (DR) is a frequent microvascular complication of diabetes mellitus, and inflammatory pathways have been linked to its pathogenesis. In this retrospective, observational pilot study, we aimed to compare the concentrations of four inflammation-related proteins, ZAG, Reg-3a, elafin and RBP-4, in the serum and aqueous humor of healthy controls and diabetic patients with different stages of DR. The concentrations of VEGF-A, IL-8, IL-6 were determined in parallel as internal controls. In the serum, we did not find significant differences in the concentrations of target proteins. In the aqueous humor, higher levels of ZAG, RBP-4, Reg-3a and elafin were observed in advanced nonproliferative DR (NPDR)/ proliferative DR (PDR) compared to controls. The levels of ZAG and RBP-4 were also higher in advanced NPDR/PDR than in nonapparent DR. Normalization of target protein concentrations to the aqueous humor total protein demonstrates that a spill-over from serum due to breakage of the blood-retina barrier only partially accounts for increased inflammation related markers in later stages. In conclusion, we found elevated levels of Reg-3a, RBP-4, elafin and ZAG in advanced stages of diabetic retinopathy. Higher levels of pro-inflammatory proteins, Reg-3a and RBP-4, might contribute to the pathogenesis of diabetic retinopathy, as the parallel increased concentrations of anti-inflammatory molecules elafin and ZAG might indicate a compensatory mechanism.

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“…44 Alanine, aspartate, and glutamate metabolism is a highly important metabolic pathway in ECs. 45 Treatment with MPs significantly impacted amino acid metabolism, which could result in vascular dysfunction. Recently, it has been demonstrated that sphingolipid levels indicate damage to heart and blood vessels in cardiometabolic diseases.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis reveals size-dependent toxicity and vascular endothelial cell injury induced by microplastic exposurein vivoandin vitro

Zhang

Shi

Huang

et al. 2022

Environ. Sci.: Nano

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Retinoprotective effect of agmatine in streptozotocin-induced diabetic rat model: avenues for vascular and neuronal protection

Cited by 14 publications

References 37 publications

Melatonin epigenetic potential on testicular functions and fertility profile in varicocele rat model is mediated by silent information regulator 1

Melatonin epigenetic potential on testicular functions and fertility profile in varicocele rat model is mediated by silent information regulator 1

Association of inflammation-related markers and diabetic retinopathy severity in the aqueous humor, but not serum of type 2 diabetic patients

Multi-omics analysis reveals size-dependent toxicity and vascular endothelial cell injury induced by microplastic exposurein vivoandin vitro

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