Multi-omics analysis reveals size-dependent toxicity and vascular endothelial cell injury induced by microplastic exposurein vivoandin vitro

Abstract

Microplastics (MPs) pollution has gained increasing attention recently. Fewer studies have examined the effects of these small items on the vascular system. The aim of this work was to precisely...

Impact of Micro- and Nanoplastics on Mitochondria

“…MNPs cause endothelial cell dysfunction by diminishing mitochondrial function and increasing pro-inflammatory cytokines, thereby triggering apoptosis [ 64 ]. This study also revealed differences in gene expression and metabolite levels between MNP-treated endothelial cells.…”

Long‐Chain Acyl Carnitines Aggravate Polystyrene Nanoplastics‐Induced Atherosclerosis by Upregulating MARCO

“…[18] In recent studies, multiomics analysis has revealed that MNP exposure induced size-dependent toxicity and vascular endothelial cell injury both in vivo and in vitro. [19,20] However in those studies, the mice were exposed to NPs by subcutaneous injection with 100 mg kg −1 of 20 nm polystyrene NPs (PS-NPs). [19,20] Those studies are detached from reality in that the MNP exposure routes and levels they examined were relevant to neither humans nor the environment in which we live.…”

“…[19,20] However in those studies, the mice were exposed to NPs by subcutaneous injection with 100 mg kg −1 of 20 nm polystyrene NPs (PS-NPs). [19,20] Those studies are detached from reality in that the MNP exposure routes and levels they examined were relevant to neither humans nor the environment in which we live. In addition, investigation into the mechanisms of PS-NP-induced vascular endothelial cell toxicity have been unsatisfactory.…”

Contaminants of emerging concern in the fetal environment

“…In HUVEC cells, cytotoxicity was observed after exposure to 50 nm PS particles with a concentration of 50 µg/mL [165][166][167] . In addition, all sizes (20-10000 nm, 1000 µg/mL) resulted in apoptosis, with higher apoptosis rates observed in cells treated with smaller PS particles 167 . Furthermore, exposure to 20, 100 and 10000 nm (1000 µg/mL) PS particles resulted in lactate dehydrogenase (LDH) leakage.…”

“…Also, placental accumulation and potential toxic effects could be investigated by using various markers of toxicity and multi-omics approaches, such as RNA sequencing and metabolomic profiling 167,188 . However, taking into account a large number of possible experimental conditions (the multitude of MNP polymer types, shapes and sizes), the most important consideration for tissue explant cultures and placental perfusion studies is the low throughput of these methods: they are laborious, time-consuming, and, morphological and functional characterization of these fragile tissues has to be performed at different time points to ensure robust results.…”