Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice

Bando, Yasuhiko; Yamamoto, Miyuki; Sakiyama, Kazuo; Sakashita, Hide; Taira, Fuyoko; Miyake, Genki; Iseki, Shoichi; Owada, Yuji; Amano, Osamu

doi:10.1007/s00418-017-1578-0

Cited by 14 publications

(12 citation statements)

References 33 publications

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“…The septoclastic processes in cartilages under active (Bando et al 2014;. The reduction in the GP cartilage resorption is induced by loose contact between transverse septa and morphologically abnormal septoclastic processes in tibiae of toothless rats (Gartland et al 2009) and in tibiae of retinoic acid-excessive or deficient mice (Bando et al 2017). Septoclasts in the present FABP5-deficiency mutant tibiae had shorter processes than those of WT control in a way similar to septoclasts in tibiae of vitamin A-deficiency mice, in which the activity of septoclasts to resorb cartilages is reduced (Bando et al2017).…”

Section: Discussionmentioning

confidence: 99%

“…Apexes of the long processes from the septoclastic bodies are attached to the uncalcified transverse septa, and therefore, septoclasts are considered to be involved in the cleavage of the transverse septa of the GP (Schenk et al1967;Lee et al 1995;Odgren et al 2016). A shortening of septoclastic processes and a decrease in their number lead septoclasts to diminish the mechanical linkage between the cells and the transverse septa, resulting in the reduction of the cleavage of the transverse septa in the GP (Bando et al 2014(Bando et al , 2017.…”

Section: Introductionmentioning

confidence: 99%

“…We previously revealed the exclusive expression of FABP5, epidermal-type FABP, in septoclasts along the COJ (Bando et al 2014). FABP5 was suggested to mediate the transport of long chain fatty acid to mitochondria for energy metabolism in septoclasts (Bando et al 2014) and the transport of retinoic acid to nuclei for induction of signaling pathways mediated by PPARβ/δ, resulting in maintenance of proliferation and morphology of septoclasts (Bando et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

Bando

Tokuda

Ogasawara

et al. 2021

Histochem Cell Biol

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In our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5−/−) mice. Those finding lead us to hypothesize that another FABP can compensate the deletion of FABP5 in septoclasts of its gene-mutant mice. Based on the hypothesis, the present study examined the expression levels of several other FABPs in septoclasts and their morphology in FABP5−/− mouse tibiae. Processes of FABP5−/− septoclasts tend to be shorter than wild septoclasts. FABP4-positive septoclasts in FABP5−/− mice were more numerous than those cells in wild mice.Peroxisome proliferator-activated receptor (PPAR) γ was expressed in FABP4-positive septoclasts of FABP5−/− mice as well as mice administered with GW1929, a PPARγ agonist, suggesting that the occurrence of PPARγ induces an increase of FABP4-positive septoclasts. The present finding suggests that the functional exertion of FABP5 in septoclasts is supplemented by FABP4 in normal and FABP5−/− mice, and that the expression of FABP4 is up-regulated in accompany with PPARγ in FABP5−/− for maintenance of resorptive activity in the GP.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

Bando

Tokuda

Ogasawara

et al. 2021

Histochem Cell Biol

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“…Fatty-acid binding proteins, such as FABP5 (also known as E-FABP or Mal1), are intracellular carriers for fatty acids and lipids and have been shown to play a surprisingly diverse role in multiple cellular processes including important roles in metabolism (Hotamisligil and Bernlohr, 2015), neurogenesis (Matsumata et al , 2016), inflammation (Berger et al , 2012; Gally et al , 2013; Bogdan et al , 2018), and cancer (Levi et al , 2013). FABP5 acts through both binding and transport of fatty acids and other lipids such as retinoic acid (Schug et al , 2007; Bando et al , 2014, 2017), and as an indirect regulator of gene expression through interactions with the PPAR class of nuclear receptors (Berger et al , 2012; Gally et al , 2013; Thumser et al , 2014).…”

Section: Discussionmentioning

confidence: 99%

Fatty-acid binding protein 5 modulates the SAR1 GTPase cycle and enhances budding of large COPII cargoes

Melville

Gorur

Schekman

2019

MBoC

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COPII-coated vesicles are the primary mediators of ER-to-Golgi trafficking. Sar1, one of the five core COPII components, is a highly conserved small GTPase, which, upon GTP binding, recruits the other COPII proteins to the ER membrane. It has been hypothesized that the changes in the kinetics of SAR1 GTPase may allow for the secretion of large cargoes. Here we developed a cell-free assay to recapitulate COPII-dependent budding of large lipoprotein cargoes from the ER. We identified fatty-acid binding protein 5 (FABP5) as an enhancer of this budding process. We found that FABP5 promotes the budding of particles ∼150 nm in diameter and modulates the kinetics of the SAR1 GTPase cycle. We further found that FABP5 enhances the trafficking of lipoproteins and of other cargoes, including collagen. These data identify a novel regulator of SAR1 GTPase activity and highlight the importance of this activity for trafficking of large cargoes.

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“…Retinoic acid is known to be bound by E-FABP and is normally present in the growth plate cartilage. Now Bando et al (2017) show that under experimental conditions of excess amounts of retinoic acid or of vitamin A deficiency, the number of E-FABP-and peroxisome-proliferator-activated receptorpositive septoclasts is significantly decreased which is due to increased apoptosis. Under conditions of excess amounts of retinoic acid, septoclasts also had shorter and fewer cellular processes, which are cell surface specializations important for the resorption of the transverse septa of the growth plate cartilage.…”

Section: Retinoic Acid Regulates Growth Plate Cartilage Septoclast Bimentioning

confidence: 99%

In focus in HCB

Taatjes

Roth

2017

Histochem Cell Biol

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thus offers a novel means to monitor and image fluorescently labeled neurons in deep regions of an awake and active rat. Rotenone effect on peroxisomal dynamics mediated by microtubulesRedox biology is an area of great interest for cell biological responses in health and disease (Little et al. 2017). Two major cellular organelles involved in reactive oxygen species (ROS) signaling are mitochondria and peroxisomes. Like many intracellular organelles, these organelles are also known to interact dynamically with one another in what is referred to as the "peroxisome-mitochondria connection" (Schrader et al. 2015). Although effects of the generation of oxidative stress in peroxisomes on mitochondrial function and morphology are well known, very little is known concerning potential effects of mitochondrial-derived oxidative stress on peroxisomal structure or function. To address this issue, Passmore et al. (2017) have performed experiments to examine effects of the complex I inhibitor rotenone on membrane dynamics occurring between mitochondria and peroxisomes. Using fluorescence microscopy, including quantitative assessment of ROS production with H 2 DCFDA, and immunoblotting they found that rotenone treatment had substantial effects on both peroxisomes and mitochondria in COS-7 cells. With respect to peroxisomes, rotenone affected both morphology and intracellular distribution through a mechanism independent of mitochondrial generated oxidative stress, but dependent upon microtubule destabilization. Interestingly, in contrast, the opposite situation occurred with the effect of rotenone on mitochondrial morphology: these effects were dependent upon the generation of ROS, but independent of microtubule stabilization. New system for monitoring brain neuronal activity in moving ratsOne of the great challenges in live animal imaging is the accurate recording of brain neuronal activity in a free-moving animal. Although multiphoton confocal microscopy has been used successfully to image more peripheral regions of the brain (Kerr et al. 2005), deeper regions remain inaccessible. Iijima et al. (2017) have now developed a system consisting of (1) a single optical fiber for recording neuronal activity via eGFP expression (linked to gonadotropin releasing hormone), and (2) a newly designed animal cage whereby the floor rotates in response to head movements, thus minimizing animal movement-derived stress on the optical fiber, for monitoring and imaging via fluorescence structures deep in the brain. This system allowed real-time fluorescence monitoring in awake and active animals over several days. By replacing the animal's water with a mild saline solution (to induce dehydration), they found an increase in eGFP fluorescence intensity in the paraventricular nucleus region. To refine their system to allow recording from individual neurons, the authors replaced the original optical fiber with a bundle containing approximately 3000 thin optical fibers and connected to an upright fluorescence microscope. Using this higher resolution ...

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Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice

Cited by 14 publications

References 33 publications

Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

Fatty-acid binding protein 5 modulates the SAR1 GTPase cycle and enhances budding of large COPII cargoes

In focus in HCB

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