Retinoic acid negatively regulates neuropeptide Y expression in human neuroblastoma cells

Magni, Paolo; Beretta, Elena; Scaccianoce, Eugenia; Motta, Marcella

doi:10.1016/s0028-3908(99)00231-2

Cited by 25 publications

(16 citation statements)

References 31 publications

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“…This effect has been observed in the skin [16], in the digestive system [3] or in the kidney [21] but there is very little information about the effect in the nervous system and nothing on the nociceptive system. In in vitro preparations, retinoic acid seems to negatively regulate the expression of neuropeptide Y in human neuroblastoma cells and this effect involves RARs [19]. Also, retinoic acid regulates the expression of mu-opioid receptors and c-fos mRNAs in some neuroblastoma cells, the effect being very dose-dependent [13].…”

Section: Discussionmentioning

confidence: 99%

The oral administration of retinoic acid enhances nociceptive withdrawal reflexes in rats with soft-tissue inflammation

et al. 2004

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Section: Discussionmentioning

confidence: 99%

The oral administration of retinoic acid enhances nociceptive withdrawal reflexes in rats with soft-tissue inflammation

et al. 2004

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“…On the molecular level, this selective NPY regulation could be due to transcription factors acting directly on the NPY promoter such as NGFI-A, AP-1, AP-2, and retinoic acid, which might act with different potencies on the promoter of the NPY gene in the two strains (Buckland et al, 2004;Jalava and Mai, 1994;Li et al, 2000;Magni et al, 2000;Wernersson et al, 1998). Moreover, it is conceivable that there is a set of factors controlling basal transcription and another set that is induced after running.…”

Section: Discussionmentioning

confidence: 99%

Running has Differential Effects on NPY, Opiates, and Cell Proliferation in an Animal Model of Depression and Controls

Bjørnebekk

Mathé

Brené

2005

Neuropsychopharmacol

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Physical activity has documented beneficial effect in treatment of depression. Recently, we found an antidepressant-like effect of running in an animal model of depression, the Flinders Sensitive Line (FSL) and demonstrated that it was associated with increased hippocampal cell proliferation. In this study, we analyzed levels of mRNAs encoding the neuropeptide Y (NPY) and the opioid peptides dynorphin and enkephalin in hippocampus and correlated these to cell proliferation in the FSL and in the 'nondepressed' Flinders Resistant Line (FRL) strain, with/without access to running wheels. Running increased NPY mRNA in dentate gyrus and the CA4 region in FSL, but not in FRL rats. NPY mRNA increase was correlated to increased cell proliferation in the subgranular zone of dentate gyrus. Baseline dynorphin and enkephalin mRNA levels in the dentate gyrus were lower in the FSL compared to the FRL strain. Running had no effect on dynorphin and enkephalin mRNAs in the FSL strain but it decreased dynorphin mRNA, and there was a trend to increased enkephalin mRNA in the FRL rats. Thus, it would appear that the CNS effects of running are different in 'depressed' and control animals; modification of NPY, a peptide associated with depression and anxiety, in depressed animals, vs effects on opioids, associated with the reward systems, in healthy controls. Our data support the hypothesis that NPY neurotransmission in hippocampus is malfunctioning in depression and that antidepressive treatment, in this case wheel running, will normalize it. In addition, we also show that the increased NPY after running is correlated to increased cell proliferation, which is associated with an antidepressive-like effect.

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“…Human neuroblastoma cells, SH-SY-5Y and SK-N-MC, contain primarily mature cathepsin V (ϳ24 kDa) and preprocathepsin V (Fig. 6, c and d); these neuronal cells contain NPY and enkephalin, respectively (29,30,33,34).…”

Section: Cellular Cathepsin V Expression Generates (Met)enkephalinmentioning

confidence: 99%

Human Cathepsin V Protease Participates in Production of Enkephalin and NPY Neuropeptide Neurotransmitters

Funkelstein

Koch

et al. 2012

Journal of Biological Chemistry

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Background: Proteases are required to generate peptide neurotransmitters. Results: Human cathepsin V participates in the production of enkephalin and NPY peptide neurotransmitters illustrated by gene expression and silencing. Conclusion: Human cathepsin V participates in producing enkephalin and NPY neurotransmitters in secretory vesicles. Significance: Elucidation of human-specific proteases participating in peptide neurotransmitter production is essential for understanding human health and disease.

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Retinoic acid negatively regulates neuropeptide Y expression in human neuroblastoma cells

Cited by 25 publications

References 31 publications

The oral administration of retinoic acid enhances nociceptive withdrawal reflexes in rats with soft-tissue inflammation

The oral administration of retinoic acid enhances nociceptive withdrawal reflexes in rats with soft-tissue inflammation

Running has Differential Effects on NPY, Opiates, and Cell Proliferation in an Animal Model of Depression and Controls

Human Cathepsin V Protease Participates in Production of Enkephalin and NPY Neuropeptide Neurotransmitters

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