Retinal Pigment Epithelial Cells Produce Fibronectin

Abstract

Antichick fibronectin antiserum, noncross-reactive to bovine fibronectin, was prepared to determine the production of fibronectin by cultured chick retinal pigment epithelial cells which were grown in the presence of fetal bovine serum. The typical fibrillary net pattern of fibronectin was observed by an indirect immunofluorescent technique when this specific antiserum reacted with cultured chick retinal pigment epithelial cells. Cultured chick retinal pigment epithelial cells were shown to produce fibronectin.

Homologous serum‐stimulated fibronectin synthesis in human retinal pigmented epithelial cells

“…Fibronectin and fibronectin mRNA expression have been recorded in subretinal membranes from patients with proliferative vitreoretinopathy (PVR), 1 which indicates that fibronectin may contribute to the course of PVR. RPE cells are a major cellular component of PVR membranes 2 and synthesize fibronectin 3 . Breakdown of the blood–retinal barrier is a fundamental feature of PVR and the relationship between plasma leakage and expression of fibronectin by RPE cells has not been previously studied.…”

“…RPE cells are a major cellular component of PVR membranes 2 and synthesize fibronectin. 3 Breakdown of the blood-retinal barrier is a fundamental feature of PVR and the relationship between plasma leakage and expression of fibronectin by RPE cells has not been previously studied. This study investigated serum-induced fibronectin expression and synthesis by RPE cells.…”

Effects of TGF-Beta on Retinal Pigmented Epithelium in vitro

“…Embryonic chick retinal pigmented epi thelial (RPE) cells grown on glass synthesize fibronectin [1] and deposit it onto the sub stratum [1][2][3][4]. The deposition of fibronectin-containing extracellular matrix (ECM) onto uncoated glass is carried out only by RPE cells which maintain the differentiated phenotype [5].…”