Homologous serum‐stimulated fibronectin synthesis in human retinal pigmented epithelial cells

Stocks, Shelli Z.; Shiels, Ian A.; Taylor, Stephen M.

doi:10.1046/j.1440-1606.1999.00199.x

Cited by 4 publications

(2 citation statements)

References 5 publications

(7 reference statements)

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“…Although we didn't detect fibronectin in healthy RPE, other studies showed that fibronectin is expressed by RPE cells and promotes the adhesion of RPE to the ECM [54], [55]. Furthermore, an increased expression and secretion of fibronectin by RPE cells was demonstrated in presence of human serum [56]. Vascular leakage is a characteristic incident that occurs with autoimmune uveitis-related breakdown of the blood-retinal barrier [28].…”

Section: Discussionmentioning

confidence: 65%

Osteopontin and Fibronectin Levels Are Decreased in Vitreous of Autoimmune Uveitis and Retinal Expression of Both Proteins Indicates ECM Re-Modeling

et al. 2011

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Autoimmune uveitis is an intraocular inflammation that arises through autoreactive T-cells attacking the inner eye, eventually leading to blindness. However, the contributing molecular pathomechanisms within the affected tissues remain as yet elusive. The extracellular matrix (ECM) is a highly dynamic structure that varies tremendously and influences the encompassing tissue. In order to assess ECM re-modeling in autoimmune uveitis, we investigated the expression of ECM molecules fibronectin and osteopontin in vitreous and retina samples. This was carried out in the only spontaneous animal model for human autoimmue uveitis, namely equine recurrent uveitis (ERU) that resembles the human disease in clinical as well as in immunopathological aspects. ERU is a naturally occurring autoimmune disease in horses that develops frequently and has already proved its value to study disease-related pathomechanisms. Western blot analysis of fibronectin and osteopontin in healthy and uveitic vitreous revealed significant reduction of both proteins in uveitis. Immunohistochemical expression of fibronectin in healthy retinas was restricted to the inner limiting membrane abutting vimentin positive Müller cell endfeet, while in uveitic sections, a disintegration of the ILM was observed changing the fibronectin expression to a dispersed pattern extending toward the vitreous. Retinal expression of osteopontin in control tissue was found in a characteristic Müller cell pattern illustrated by co-localization with vimentin. In uveitic retinas, the immunoreactivity of osteopontin in gliotic Müller cells was almost absent. The ability of Müller cells to express fibronectin and osteopontin was additionally shown by immunocytochemistry of primary cultured equine Müller cells and the equine Müller cell line eqMC-7. In conclusion, severe ECM re-modeling in autoimmune uveitis reported here, might affect the adhesive function of fibronectin and thus the anchoring of Müller cell endfeet to the ILM. Furthermore, the absence of osteopontin in gliotic Müller cells might represent reduced neuroprotection, an osteopontin attribute that is intensively discussed.

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Section: Discussionmentioning

confidence: 65%

Osteopontin and Fibronectin Levels Are Decreased in Vitreous of Autoimmune Uveitis and Retinal Expression of Both Proteins Indicates ECM Re-Modeling

et al. 2011

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“…However, we only observed reduced invasiveness upon treatment with human serum, indicating that invasion-inhibitory factors are host specific and not found in bovine serum. Such human serum-specific effects have been previously reported: human but not bovine serum can inhibit apoptosis, membrane permeabilization and the release of chemokines in epithelial cells [31]; human, but not bovine, serum stimulates the expression of fibronectin by epithelial cells [32]. Therefore, it is possible that treatment with human serum triggered an epithelial reaction, which protected these cells from fungal invasion and damage.…”

Section: Discussionmentioning

confidence: 69%

Candida albicans-Epithelial Interactions: Dissecting the Roles of Active Penetration, Induced Endocytosis and Host Factors on the Infection Process

et al. 2012

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Candida albicans frequently causes superficial infections by invading and damaging epithelial cells, but may also cause systemic infections by penetrating through epithelial barriers. C. albicans is a remarkable pathogen because it can invade epithelial cells via two distinct mechanisms: induced endocytosis, analogous to facultative intracellular enteropathogenic bacteria, and active penetration, similar to plant pathogenic fungi. Here we investigated the contributions of the two invasion routes of C. albicans to epithelial invasion. Using selective cellular inhibition approaches and differential fluorescence microscopy, we demonstrate that induced endocytosis contributes considerably to the early time points of invasion, while active penetration represents the dominant epithelial invasion route. Although induced endocytosis depends mainly on Als3-E–cadherin interactions, we observed E–cadherin independent induced endocytosis. Finally, we provide evidence of a protective role for serum factors in oral infection: human serum strongly inhibited C. albicans adhesion to, invasion and damage of oral epithelial cells.

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Transforming growth factor‐β₁ induces α‐smooth muscle actin expression and fibronectin synthesis in cultured human retinal pigment epithelial cells

Stocks¹,

Taylor

Shiels

2001

Clinical Exper Ophthalmology

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Elevated levels of TGF-beta1 in proliferative vitreoretinopathy may contribute to phenotype changes in retinal pigment epithelial cells leading to matrix deposition and contraction. Since the elevated levels of TGF-beta1 may emanate from a number of diverse sources in proliferative vitreoretinopathy, developing an antagonist to TGF-beta1 may offer an approach to the treatment of proliferative vitreoretinopathy.

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Homologous serum‐stimulated fibronectin synthesis in human retinal pigmented epithelial cells

Abstract: The results show that human serum contains substances stimulating fibronectin synthesis in human RPE cells.

Cited by 4 publications

References 5 publications

Osteopontin and Fibronectin Levels Are Decreased in Vitreous of Autoimmune Uveitis and Retinal Expression of Both Proteins Indicates ECM Re-Modeling

Osteopontin and Fibronectin Levels Are Decreased in Vitreous of Autoimmune Uveitis and Retinal Expression of Both Proteins Indicates ECM Re-Modeling

Candida albicans-Epithelial Interactions: Dissecting the Roles of Active Penetration, Induced Endocytosis and Host Factors on the Infection Process

Transforming growth factor‐β₁ induces α‐smooth muscle actin expression and fibronectin synthesis in cultured human retinal pigment epithelial cells

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Homologous serum‐stimulated fibronectin synthesis in human retinal pigmented epithelial cells

Abstract: The results show that human serum contains substances stimulating fibronectin synthesis in human RPE cells.

Cited by 4 publications

References 5 publications

Osteopontin and Fibronectin Levels Are Decreased in Vitreous of Autoimmune Uveitis and Retinal Expression of Both Proteins Indicates ECM Re-Modeling

Osteopontin and Fibronectin Levels Are Decreased in Vitreous of Autoimmune Uveitis and Retinal Expression of Both Proteins Indicates ECM Re-Modeling

Candida albicans-Epithelial Interactions: Dissecting the Roles of Active Penetration, Induced Endocytosis and Host Factors on the Infection Process

Transforming growth factor‐β1 induces α‐smooth muscle actin expression and fibronectin synthesis in cultured human retinal pigment epithelial cells

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Transforming growth factor‐β₁ induces α‐smooth muscle actin expression and fibronectin synthesis in cultured human retinal pigment epithelial cells