Retinal Nerve Fiber Layer and Macula Thickness with Spectral Domain Optical Coherence Tomography in Children: Normal Values, Repeatability and the Inflfluence of Demographic and Ocular Parameters

Abstract

A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To determine normal values for retinal nerve fiber layer (RNFL) and macular thickness in children using spectral domain optical coherence tomography (SD-OCT), to assess the repeatability of the SD-OCT measurements and to analyse their correlations with age, gender, refraction and axial length. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Healthy children aged 5-17 years were enrolled for this cross-sectional study. All subjects underwent comprehensive ophthalmological examination, cycloplegic refraction and axial length measurement. Thereafter, three consecutive SD-OCT measurements were obtained by the same operator. The mean of three consecutive measurements for each SD-OCT parameter was included in the normal values. Repeatability was calculated by comparing the three consecutive measurements and expressed as an intraclass correlation coefficient. The correlation of all SD-OCT parameters with age, gender, spherical equivalent refraction and axial length were statistically analysed. R Re es su ul lt ts s: : Two hundred two eyes of 202 children (103 females and 99 males) with a mean age of 10.4±3.4 years were included. The mean of average RNFL thickness was 103.9±8.2 (84.3-132) µm. The mean central macular thickness was 257.4± 22.1 (217-329) µm. Intraclass correlation coefficients of repeated measurements were over 0.81 for all evaluated parameters. Males had significantly thicker central macula than females (p=0.013). On multivariate linear regression analysis, spherical equivalent refraction was the only parameter independently effective on average RNFL thickness (p<0.001). C Co on nc cl lu us si io on n: : This study has provided normal values for RNFL and macular thickness measured with SD-OCT in healthy children. The high repeatability of the measurements indicates that SD-OCT can be used reliably in children that are five years and older. Data obtained in this study may be helpful in the assessment of childhood optic nerve and macular diseases with SD-OCT. K Ke ey yw wo or rd ds s: : Child; tomography, optical coherence; macula lutea; retina Ö ÖZ ZE ET T A Am ma aç ç: : Çocuklarda spektral domain optik koherens tomografi (SD-OKT) ile retina sinir lifi (RSLT) ve maküla kalınlıklarının normal değerlerini belirlemek, SD-OKT ölçümlerinin tekrarlanabilirliğini ve yaş, cinsiyet, refraksiyon ve aksiyel uzunluk ile ilişkilerini saptamak. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu kesitsel çal-ışmaya yaşları 5-17 yıl arasında değişen sağlıklı çocuklar dahil edildi. Tüm olguların ayrıntılı göz muayenesi, sikloplejik refraksiyon ve aksiyel uzunluk ölçümleri yapıldı. Sonrasında art arda üç kez aynı görevli tarafından SD-OKT ölçümleri alındı. Normal değerler belirlenirken her parametre için bu üç ölçümün ortalaması alındı. Tekrarlanabilirlik ise bu üç ölçüm karşılaştırılarak hesaplandı ve sınıf içi korelasyon katsayısı olarak ifade edildi. Ayrıca tüm SD-OKT parametrelerinin yaş, cinsiyet, sferik ekivalan refraksiyon ve aksiyel uzunluk...