Retinal Angiogenesis Effects of TGF-β1 and Paracrine Factors Secreted from Human Placental Stem Cells in Response to a Pathological Environment

Kim, Kyung-Sul; Park, Ji-Min; Kong, TaeHo; Kim, Chul; Bae, Sang-Hun; Kim, Han Wool; Moon, Jisook

doi:10.3727/096368915x688263

Cited by 49 publications

(60 citation statements)

References 45 publications

(46 reference statements)

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“…The hallmark of DR is progressive microvascular disease, in which abnormal proliferation of retinal angiogenesis in DR progression is an important pathological change. Retinal blood vessels normally do not penetrate through the inner limiting membrane, so those vessels that break through the inner membrane can be considered as newly generated vessels of the retina.…”

Section: Discussionmentioning

confidence: 99%

Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model

Wang

Lan

Liao

et al. 2018

J of Diabetes Invest

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Aims/Introduction To investigate the protective effect of Polygonatum sibiricum polysaccharide ( PSP ) on the retina in diabetic rats. Materials and Methods A total of 120 Sprague–Dawley rats were randomly divided into blank control, control model (meaning diabetes mellitus), and diabetes mellitus with PSP intervention of high, medium and low doses groups. The difference of retinal vascularization between groups was evaluated by fluorescein isothiocyanate‐dextran perfusion. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining was used to assess apoptosis in the retinal ganglion cells; reverse transcriptase polymerase chain reaction and western blotting were utilized to evaluate the expression of Bcl2‐associated X protein, B‐cell lymphoma‐2 factor, epidermal growth factor, p38 mitogen‐activated protein kinases, transforming growth factor‐β and vascular endothelial growth factor at the messenger ribonucleic acid and protein level. Results Fluorescein isothiocyanate‐dextran perfusion showed retinal vascular anomaly in diabetes mellitus rats, but vascular tortuosity and leakage were relatively alleviated after PSP intervention. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining showed numerous terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling‐positive retinal cells in the diabetes mellitus group, which then were reduced by PSP treatment. Reverse transcriptase polymerase chain reaction showed that PSP intervention decreased Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β messenger ribonucleic acid expression, but increased B‐cell lymphoma‐2 factor messenger ribonucleic acid expression. Western blot showed that PSP intervention upregulated the expression of B‐cell lymphoma‐2 factor, and downregulated the expression of Bcl2‐associated X protein, epidermal growth factor, p38 mitogen‐activated protein kinases, vascular endothelial growth factor and transforming growth factor‐β proteins. Conclusions Polygonatum sibiricum polysaccharide shows a protective effect against diabetes‐induced retinal injury in a dose‐dependent manner. The mechanism of action deserves further study and exploration.

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Section: Discussionmentioning

confidence: 99%

Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model

Wang

Lan

Liao

et al. 2018

J of Diabetes Invest

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“…There are no effective measures to promote nerve repair after DR nerve damage, and exogenous cell replacement therapy may be a potential treatment for the damage 9 . NSCs have become a promising source of stem cell-based regenerative therapy.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy

Zhang

Wang

Kong

et al. 2017

Sci Rep

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The aim of this study was to evaluate the effects of intravitreal injection of neural stem cells (NSCs) originating from human umbilical cord-derived mesenchymal stem cells (UC-MSCs) on neurodegeneration of diabetic retinopathy (DR) in rats. UC-MSCs were isolated and passaged, followed by induction to NSCs in neural differentiation medium. Four weeks following NSC transplantation, treatment attenuated retinal vascular dysfunction compared with non-treated rats, and BDNF and Thy-1 expression was significantly higher in the treated group than in the control group. Treatment of diabetic rats with NSCs prevented the decrease in BDNF levels caused by diabetes. The average leakage of Evans Blue (EB) dye in the treated group was significantly less than that in the control group. These morphological improvements were accompanied by a restoration of vision, as documented by F-ERG. NSCs originating from MSCs demonstrated a neuroprotective effect by increasing the number of surviving RGCs and significantly reducing the progression of DR. Thus, transplantation of NSCs could be a novel strategy for the treatment of neurodegeneration in DR.

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“…Wang et al [121] and Zhao et al [122] revealed that the area of retinal neovascularization was significantly smaller in OIR mice with intravitreally injected BM-MSCs on postnatal day 12; the injected MSCs were located in retinal neovascularized areas but did not integrate into the vascular network. Kim et al [123] showed that human placental amniotic membrane-derived MSCs (AM-MSCs) secreted high levels of TGF-β1 and suppressed endothelial cell proliferation under pathological conditions in vitro. Moreover, TGF-β1 siRNA nullified the benefits of MSCs both in vivo and in vitro; TGF-β1 is a key secretory factor in the regulation of MSC-mediated angiogenesis [123].…”

Section: Msc-derived Secretomes For Ropmentioning

confidence: 99%

“…Kim et al [123] showed that human placental amniotic membrane-derived MSCs (AM-MSCs) secreted high levels of TGF-β1 and suppressed endothelial cell proliferation under pathological conditions in vitro. Moreover, TGF-β1 siRNA nullified the benefits of MSCs both in vivo and in vitro; TGF-β1 is a key secretory factor in the regulation of MSC-mediated angiogenesis [123]. Another study described the protective effects of human BM-MSC-derived exosomes against ROP [76].…”

Section: Msc-derived Secretomes For Ropmentioning

confidence: 99%

Mesenchymal stem cell-derived secretomes for therapeutic potential of premature infant diseases

et al. 2020

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Preterm birth is a complex syndrome and remains a substantial public health problem globally. Its common complications include periventricular leukomalacia (PVL), bronchopulmonary dysplasia (BPD), necrotizing enterocolitis (NEC) and retinopathy of prematurity (ROP). Despite great advances in the comprehension of the pathogenesis and improvements in neonatal intensive care and associated medicine, preterm birth-related diseases remain essentially without adequate treatment and can lead to high morbidity and mortality. The therapeutic potential of mesenchymal stem/stromal cells (MSCs) appears promising as evidenced by their efficacy in preclinical models of pathologies relevant to premature infant complications. MSC-based therapeutic efficacy is closely associated with MSC secretomes and a subsequent paracrine action response to tissue injuries, which are complex and abundant in response to the local microenvironment. In the current review, we summarize the paracrine mechanisms of MSC secretomes underlying diverse preterm birth-related diseases, including PVL, BPD, NEC and ROP, are summarized, and focus is placed on MSC-conditioned media (CM) and MSC-derived extracellular vesicles (EVs) as key mediators of modulatory action, thereby providing new insights for future therapies in newborn medicine.lung function and help to remove the ventilator as soon as possible [12]. However, many studies have reported that glucocorticoid can increase the risk of adverse reactions, such as those by the nervous system, in the early or late stages, and its dose and course of treatment are not certain [13,14]. In terms of surgical treatment, preterm infants with surgical NEC are at risk of significant growth reduction and neurodevelopmental impairment (NDI), including cerebral palsy, deafness and blindness [15,16]. These sequelae and complications of prematurity-related diseases affect not only the neonatal period, but also infancy and childhood, and even adolescence and adulthood [17][18][19]. Currently, although various treatment methods have been found to achieve certain curative effects, there still exist several side effects and disputes above. Thus, it is necessary to explore and develop new therapies for the treatment and prevention of premature infant diseases.Accumulating studies have established the importance and feasibility of stem cell-based therapies to ameliorate many degenerative and inflammatory diseases. Among these stem/progenitor cell types, mesenchymal stem/stromal cells (MSCs) have received more extensive attention because they are easily extracted, exert anti-inflammatory effects, have low immunogenicity and can renew themselves. The therapeutic potential of MSCs has been demonstrated in many diseases such as cancer [20,21] and cardiovascular [22,23] and lung [24][25][26] diseases. MSCs also represent a promising and growing approach to the targeting of various pathological processes and cellular impairments underlying the major abnormalities in premature infant and preterm-associated neonatal diseases [27][28]...

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Retinal Angiogenesis Effects of TGF-β1 and Paracrine Factors Secreted from Human Placental Stem Cells in Response to a Pathological Environment

Cited by 49 publications

References 45 publications

Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model

Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model

Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy

Mesenchymal stem cell-derived secretomes for therapeutic potential of premature infant diseases

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