Retina damage after exposure to UVA radiation on the early developmental stages of the Egyptian toad Bufo regularis Reuss

Abstract

The present study was carried out to investigate the histological and histochemical changes in the retina on different developmental stages of Egyptian toad Bufo regularis. Our experiment started when tadpoles begin to feed. The adapted embryos are divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Both histological and histochemical results showed that the general architecture of the retina organ is correlated with the state of development. Therefore, it displayed different characteristic features depending on the investigated developmental stage starting from the larval stage (feeding began, stage 44) and ending with the postmetamorphic stage 66. Also, the present work aimed to study the chronic effects of UVA on the retina structure of B. regularis during development and metamorphosis for the first time.

Xenopus: An in vivo model for studying skin response to ultraviolet B irradiation

“…To investigate the cellular responses to UVB, several models have been used including monolayer cells, skin equivalent, skin explant, and mouse models (Cario‐Andre et al, 2000; Cario‐Andre et al, 2002; Duval et al, 2001; Mahfouf et al, 2019; Raad et al, 2017; Rezvani et al, 2006; Rezvani et al, 2007; Scott, 2002). Besides mouse as the favorite mammalian model, several aquatic species, ranging from crustacean and echinoderm to different species of fish and amphibian, have been used to investigate the effects of UV radiation on development and behavior (Blaustein et al, 1997; Bonaventura et al, 2021; de Quadros et al, 2016; Dong et al, 2007; Hurem et al, 2018; Mahmoud et al, 2009; Nahon et al, 2009; Sayed et al, 2013; Sayed et al, 2016; Smith et al, 2000). Of note, increasing UVB radiation due to ozone layer depletion and consequently persistent UVB‐induced damage have been proposed as a potential cause for the disappearance of amphibian populations (Blaustein & Belden, 2003).…”