Retention of Nanoparticles-Labeled Bone Marrow Mononuclear Cells in the Isolated <i>Ex Vivo</i> Perfused Heart After Myocardial Infarction in Animal Model

Klabusay, Martin; Scheer, Peter; Doubek, Michael; Řeháková, Kristína; Čoupek, Petr; Horký, Drahomír

doi:10.3181/0803-rm-109

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…The use of injection anaesthesia with diazepam, xylazine and ketamine is well validated in our experimental practice, in both rats and rabbits, and in a large spectrum of cases (pulmonary embolism, septic shock, myocardial infarction; Dembovska et al, 2008;Klabusay et al, 2009). The combination of diazepam, xylazine, and ketamine reported here is both reasonably priced and readily available worldwide.…”

Section: Discussionmentioning

confidence: 99%

Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats

Scheer¹,

Sverakova²,

Doubek³

et al. 2012

Vet. Med. - Czech

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This paper describes the partial results of an echocardiographic study in sixty outbreed Wistar rats. Animals of parity sex ratio were chosen for the experiment. The animals were grown up during the observation period (the minimum weight was 220 g; the maximum weight was 909 g) and were then sequentially anaesthetised (2-2.5% of isoflurane, 3 l/min O 2 ). The second, fourth and fifth examinations were performed under anaesthesia maintained by intramuscular injections with diazepam (2 mg/kg), xylazine (5 mg/kg) and ketamine (35 mg/kg). Transthoracal examination was done using the SonoSite Titan echo system (SonoSite Ltd.) with a microconvex transducer C11 (8-5 MHz). M-mode (according to the leading-edge method of American Society of Echocardiography) echocardiography data were acquired at the papillary muscle: systolic and diastolic interventricular septum (IVSs, d) and left vetricular posterior wall (LVPWs, d) thickness, systolic and diastolic left ventricular dimension (LVDs, d), aorta (Ao) and left atrium (LA) dimensions. According to standard formulas, the following parameters were obtained: ejection fraction (EF), cardiac output (CO), stroke volume (SV), left ventricle end systolic volume (LVESV), left ventricle end diastolic volume (LVEDV), interventricular septum fractional thickening (IVSFT), left ventricular dimension fraction shortening (LVDFS), and left ventricle posterior wall fraction thickening (LVPWFS). In our study we performed 300 examinations both in male and female Wistar rats of various body weights and calculated regression equations to predict expected normal echocardiographic parameters for rats with arbitrary weights. The rats were examined by an echo scan. The first and third examinations were performed during monoanaesthesia induced by inhalation of isoflurane. Correlations, with one exception (LVDs), were very close, which means that the results of the calculations based on regression equations are very reliable.

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Section: Discussionmentioning

confidence: 99%

Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats

Scheer¹,

Sverakova²,

Doubek³

et al. 2012

Vet. Med. - Czech

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“…Aspects of BM-MNCs' interaction with the infarcted tissue (changes in morphology of the BM-MNCs, quantification of BM-MNCs' integration into the muscle, adhesion to endothelial cells and to the extracellular matrix) have been analyzed using electron and fluorescence microscopy on heart samples extracted and fixed postmortem [17][18][19]. The junctions created between BM-MNCs and CMs have also been analyzed [12] (application of cells to an extracorporeal rat beating heart model and subsequent analysis of fixed slices of the heart tissue).…”

Section: Discussionmentioning

confidence: 99%

Formation of Cell-To-Cell Connection between Bone Marrow Cells and Isolated Rat Cardiomyocytes in a Cocultivation Model

Skopalík¹

2014

J Cell Sci Ther

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Aims: Limited regenerative potential of cardiomyocytes (CMs) causes irreversible changes in heart tissue during pathological processes. However bone marrow mononuclear cells (BM-MNCs) can migrate to this tissue, incorporate to the area of dead or missing myocytes, and improve the global heart function. The mechanism of BM-MSCs' incorporation and interaction with CMs is not clear. Our aim was to create an in vitro model which would enable to study the interaction of BM-MNCs with CMs and to make a microscopy description of these interactions. Methods and Results: CMs were isolated from adult and newborn rats. BM-MNCs were isolated from bone marrow. BM-MNCs were added to the myocyte culture. Cell-to-cell adherence and Cx43 expression were evaluated by fluorescence microscopy, Ca 2+ transients were evaluated in cardiomyocyte-BMC communication under electrical stimulation by fluo-4 fluorescence measurement. Analysis of calcein transport from BM-MNCs to CMs was performed using fluorescence microscopy. Conclusions: The adherence of BM-MNCs to CMs occurred quickly and was stable. Cx43 was detected in contact zones between BM-MNCs and CMs; pairs which displayed Cx43 positivity represented less than 1% from all BM-MNC-cardiomyocyte pairs in the coculture. Conductive structures between CMs and BM-MNCs were formed and verified by imaging calcein transfer and synchronous Ca 2+ transients.

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“…They are regarded as therapeutic tools with great potential, in particular for the significant and difficult zones of access such as the brain or the heart. Among these, we find neuronal cells [142], spontaneous remyelination in sclerosis [143] or cell therapy of myocardial infarction [144].…”

Section: View Of the Various Therapeutic Strategies Of Superparamagnementioning

confidence: 99%

Magnetic Iron Oxide Nanoparticles for Biomedical Applications

et al. 2010

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Due to their high magnetization, superparamagnetic iron oxide nanoparticles induce an important decrease in the transverse relaxation of water protons and are, therefore, very efficient negative MRI contrast agents. The knowledge and control of the chemical and physical characteristics of nanoparticles are of great importance. The choice of the synthesis method (microemulsions, sol-gel synthesis, laser pyrolysis, sonochemical synthesis or coprecipitation) determines the magnetic nanoparticle's size and shape, as well as its size distribution and surface chemistry. Nanoparticles can be used for numerous in vivo applications, such as MRI contrast enhancement and hyperthermia drug delivery. New developments focus on targeting through molecular imaging and cell tracking.

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Retention of Nanoparticles-Labeled Bone Marrow Mononuclear Cells in the Isolated Ex Vivo Perfused Heart After Myocardial Infarction in Animal Model

Cited by 7 publications

References 29 publications

Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats

Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats

Formation of Cell-To-Cell Connection between Bone Marrow Cells and Isolated Rat Cardiomyocytes in a Cocultivation Model

Magnetic Iron Oxide Nanoparticles for Biomedical Applications

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