Retention of Microbiota Diversity by Lactose-Free Milk in a Mouse Model of Elderly Gut Microbiota

Abstract

Prebiotics may improve ageing-related dysbiosis. Milk is a source of nutrients including oligosaccharides whose prebiotic potential remains largely unexplored. We used a murine model to explore the effect of milk products on high diversity and lower diversity faecal microbiota from healthy and frail elderly subjects, respectively. Mice were treated with antibiotics and subsequently "humanised" with human faecal microbiota. The mice received lactose-free or whole milk, glycomacropeptide, or soy protein (control) supplemented diets for one month. The faecal microbiota was analysed by 16S rRNA gene amplicon sequencing. Lactose-free milk diet was as efficient as the control diet in retaining faecal microbiota diversity in mice. Both milk diets had a significant effect on the relative abundance of healthrelevant taxa (e.g. Ruminococcaceae, Lachnospiraceae). The glycomacropeptide prebiotic activity previously observed in vitro was not replicated in vivo. However, these data indicate the novel prebiotic potential of bovine milk for human nutrition.

Effects of Regular Kefir Consumption on Gut Microbiota in Patients with Metabolic Syndrome: A Parallel-Group, Randomized, Controlled Study

“…However, Bacteroidetes group was decreased compared to the baseline. In accordance with our results, Ntemiri et al also found out that whole milk consumption was associated with an increase in taxons belonging to Firmicutes and a higher Firmicutes/Bacteroidetes ratio [76]. In another randomized cross–over study, consumption of probiotic yogurt and milk acidified with D-(+)-glucono-δ-lactone showed some distinct effects on microbiota composition.…”

“…Apart from fermentation, unfermented dairy products may also affect the gut microbiota [75,76]. In our study, regular milk consumption that was used as control also led to some changes in microbiota composition compared to the baseline.…”

Whole Blueberry and Isolated Polyphenol-Rich Fractions Modulate Specific Gut Microbes in an In Vitro Colon Model and in a Pilot Study in Human Consumers

“…The pipeline for the microbiota composition analysis was described before [48] and comprised the following steps. Paired-end reads were joined with FLASH [49] and quality filtering was performed in Qiime (v.1.9.1) using the split_libraries_fastq.py script [50].…”

Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis

“…In all, the developed pipeline avoids overlooking the less abundant species, while at the same time resulting on a better understanding of the analyzed microbial populations. Since this methodology allows for a full understanding of any 16S RNA sequencing dataset, it has the potential to unravel the dynamics of other microbial ecosystems important for the agricultural and food chemistry field, such as in food fermentation processes [8] or to study the gut microbiota [9,40].…”

“…In the study of anaerobic digestion processes, the analysis of the microbial communities can provide an insightful link between the physicochemical state of these digesters and the growth of the microorganisms [7][8][9]. Therefore, in waste AcoD, this analysis is not only useful for obtaining a microbial characterization of the digestion process but also to determine the digester conditions (e.g.…”