Retention modeling and adsorption mechanisms in reversed-phase liquid chromatography

Tsui, Hung‐Wei; Lin, Songzhu; Hsu, Y. F.; Dai, Feng-Ji

doi:10.1016/j.chroma.2021.462736

Cited by 6 publications

(5 citation statements)

References 48 publications

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“…S-shaped adsorption isotherms were found for MeCN, ACE, and iPrOH. This S-shaped isotherm curve has also been observed for MeCN adsorption on C 18 sorbent [38].…”

Section: Adsorption Isotherms Of Modifierssupporting

confidence: 73%

“…With the exception of the data obtained at high PrOH and iPrOH concentrations, the retention factors of R‐MM decreased with increasing modifier concentration (Figure S3). This finding was attributed to both weakened hydrophobic interaction in the presence of modifier molecules and competitive adsorption between the modifier and solute [38]. The strength of the modulating effect for the various modifiers increased in the following order: MeOH < EtOH < iPrOH ≈ PrOH ≈ ACE ≈ tBuOH (Figure S3).…”

Section: Resultsmentioning

confidence: 98%

“…Using neat water as the mobile phase, we discovered that the retention factors of nine simple solutes increased in the following order: MeOH < EtOH < MeCN < iPrOH < ACE < THF < BZD < BnOH < BZN (Table 1 and Figure S1), which is almost consistent with the overall molecular polarity. This retention factor ordering somewhat reflects the ordering of the adsorbate–sorbent interaction equilibrium constant K M [37, 38].…”

Section: Resultsmentioning

confidence: 99%

“…In reversed-phase mode, MeCN adsorption was reported to follow the Brunauer-Emmett-Teller (BET) multilayer isotherm [37,38,[41][42][43]. In the BET model, the adsorbatesorbent interactions are assumed to be independent of the solvent composition.…”

Section: Adsorption Isotherms Of Modifiersmentioning

confidence: 99%

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Effect of mobile‐phase modifiers on the enantioselective retention behavior of methyl mandelate with an amylose 3,5‐dimethylphenylcarbamate chiral stationary phase under reversed‐phase conditions

Tsui

Hsieh

Zhan

2022

J of Separation Science

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In this study, methanol, ethanol, n‐propyl alcohol, isopropyl alcohol, acetone, and tert‐butanol were used as organic modifiers in reversed‐phase mode chiral liquid‐chromatography to systematically investigate the effects of mobile phase components on the enantioselective retention behavior of methyl mandelate with immobilized amylose 3,5‐dimethylphenylcarbamate‐based sorbent called Chiralpak IA. A two‐site enantioselective model was used to obtain information on the recognition mechanisms by observing the dependence of the enantioselectivity and retention factor difference on the modifier content. Similar enantioselective retention behaviors were observed for all modifiers, and characteristic modifier concentration points (PL, PM, and PH) were identified. At modifier concentrations up to PM, the weakened hydrophobic environment resulted in polymer structural relaxation, which changed the recognition mechanisms. By contrast, at concentrations beyond PH, considerably different enantioselectivity behaviors were observed, indicating that the existence of dipole‐dipole interaction, which was stronger at higher modifier concentrations, contributed to the retention mechanisms. The concentrations at which these characteristic points occurred were dependent on the carbon number of the modifier molecule. Modifiers with more carbon numbers facilitated the transition in the enantioselective behaviors. These results demonstrated that the proposed method can provide a physically consistent quantitative description of enantioselective retention behavior in reversed‐phase mode.

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“…S-shaped adsorption isotherms were found for MeCN, ACE, and iPrOH. This S-shaped isotherm curve has also been observed for MeCN adsorption on C 18 sorbent [38].…”

Section: Adsorption Isotherms Of Modifierssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Adsorption Isotherms Of Modifiersmentioning

confidence: 99%

See 2 more Smart Citations

Effect of mobile‐phase modifiers on the enantioselective retention behavior of methyl mandelate with an amylose 3,5‐dimethylphenylcarbamate chiral stationary phase under reversed‐phase conditions

Tsui

Hsieh

Zhan

2022

J of Separation Science

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“…Nevertheless, occasionally challenging separation tasks cannot be accomplished on conventional alkyl stationary phases. The polar embedded alkyl stationary phases usually are superior to the conventional alkyl stationary phases in water resistance, silanol activity, and selectivity to polar compounds due to their different retention mechanisms, such as hydrogen bonding, electrostatic, π–π stacking, and steric hindrance. , Then, the retention mechanism of aromatic phases is attributed to π–π interactions between the analyte and the phenyl moieties of the stationary phase, weak electrostatic, dipole–dipole interactions with analytes and steric hindrance factors. Retention and selectivity of aromatic phases for analytes are highly complementary to alkyl equivalents …”

Section: Introductionmentioning

confidence: 99%

Grafting of Tetraphenylethylene on Silica Surface, Characterizations, and Their Chromatographic Performance as Reversed-Phase Stationary Phases

et al. 2022

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Surface modification is an effective way to functionalize the materials so as to get some special properties. Tetraphenylethylene (TPE) has been widely investigated as a well-known reagent which has the nature of aggregation-induced emission (AIE), but has never been reported in the liquid chromatography stationary phase. In this work, TPE-grafted silica (Sil-TPE) was obtained successfully using the derivative of 1-(4-hydroxyphenyl)-1,2,2-triphenylethylene as a ligand, and then characterized by elemental analysis, Fourier transform infrared spectra, thermogravimetric analysis, and so forth. Laser scanning confocal microscopy images reflected the AIE phenomenon of grafted TPE because the internal vibration and rotation of TPE molecules were restrained in the confined silica space. The contact angle test showed superhydrophobic properties of Sil-TPE. In order to understand thoroughly the mechanism of chromatographic performance and retention behavior for Sil-TPE, Tanaka test mixture, alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), and phenols were separated. This reveals that Sil-TPE has strong aromaticity and certain shape selectivity, especially, has excellent separation performance for PAHs and phenols. The thermodynamic properties and repeatability of Sil-TPE were further studied, which showed the stability of Sil-TPE. This work shows that TPE can be successfully grafted on silica surface and it has the potential to be a new kind of promising stationary phases in the future.

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Applications of the solvation parameter model in thin-layer chromatography

Poole

2022

JPC-J Planar Chromat

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Retention modeling and adsorption mechanisms in reversed-phase liquid chromatography

Cited by 6 publications

References 48 publications

Effect of mobile‐phase modifiers on the enantioselective retention behavior of methyl mandelate with an amylose 3,5‐dimethylphenylcarbamate chiral stationary phase under reversed‐phase conditions

Effect of mobile‐phase modifiers on the enantioselective retention behavior of methyl mandelate with an amylose 3,5‐dimethylphenylcarbamate chiral stationary phase under reversed‐phase conditions

Grafting of Tetraphenylethylene on Silica Surface, Characterizations, and Their Chromatographic Performance as Reversed-Phase Stationary Phases

Applications of the solvation parameter model in thin-layer chromatography

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