Retention mechanisms in micellar liquid chromatography

Ruiz-Ángel, M.J.; Carda‐Broch, Samuel; Torres-Lapası́o, J.R.; García-Álvarez-Coque, M.C.

doi:10.1016/j.chroma.2008.09.053

Cited by 147 publications

(115 citation statements)

References 64 publications

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“…On the other hand, anionic drugs are expected to show an antibinding interaction owing to electrostatic repulsion between the anionic drug molecules and the anionic micelles, which leads to binding of these drug molecules to the cyanopropyl column, increasing their retention times with the increase in the micellar concentration in the mobile phase (Armstrong & Stine, 1983; Ruiz‐Angel, Carda‐Broch, Torres‐Lapasió, & García‐Álvarez‐Coque, 2009). …”

Section: Resultsmentioning

confidence: 99%

Incorporating physiologically relevant mobile phases in micellar liquid chromatography for the prediction of human intestinal absorption

Shokry

Waters

Parkes

et al. 2018

Biomedical Chromatography

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Micellar liquid chromatography is a popular method used in the determination of a compound's lipophilicity. This study describes the use of the obtained micelle–water partition coefficient (log P mw) by such a method in the prediction of human intestinal absorption (HIA). As a result of the close resemblance of the novel composition of the micellar mobile phase to that of physiological intestinal fluid, prediction was deemed to be highly successful. The unique micellar mobile phase consisted of a mixed micellar mixture of lecithin and six bile salts, i.e. a composition matching that found in the human intestinal environment, prepared in ratios resembling those in the intestine. This is considered to be the first method to use a physiological mixture of biosurfactants in the prediction of HIA. As a result, a mathematical model with high predictive ability (R 2 PRED = 81%) was obtained using multiple linear regression. The micelle–water partition coefficient (log P mw) obtained from micellar liquid chromatography was found to be a successful tool for prediction where the final optimum model included log P mw and polar surface area as key descriptors with high statistical significance for the prediction of HIA. This can be attributed to the nature of the mobile phase used in this study which contains the lecithin–bile salt complex, thus forming a bilayer system and therefore mimicking absorption across the intestinal membrane.

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Section: Resultsmentioning

confidence: 99%

Incorporating physiologically relevant mobile phases in micellar liquid chromatography for the prediction of human intestinal absorption

Shokry

Waters

Parkes

et al. 2018

Biomedical Chromatography

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“…SDS is a fatty alcohol sulfate that is aerobically degraded. Thomas and White [20] observed that 70% of 14 C SDS was degraded to CO 2 and the remaining 30% was incorporated into the microbial biomass, i.e., 100% of the SDS was utilized for either energy or biomass production. On the other hand, Brij-35 is one of the fatty alcohol ethoxylate derivatives, developed as an eco-friendly alternative to alkyl phenol ethoxylates [21].…”

Section: Waste Generationmentioning

confidence: 99%

“…While retention of more polar compounds is determined by their partitioning from the bulk aqueous phase into micelle and alkyl stationary phase, the more hydrophobic compounds might be directly transferred from micelles in the mobile phase into the stationary phase [13,14].…”

Section: Principles Of Separation By Mlcmentioning

confidence: 99%

Micellar Liquid Chromatography from Green Analysis Perspective

2015

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Micellar liquid chromatography (MLC) is a simple well-established branch of high-performance liquid chromatography. The applications of MLC for the determination of numerous compounds in pharmaceutical formulations, biological samples, food, and environmental samples have been growing very rapidly. MLC technique has several advantages over other techniques, such as simultaneous separation of charged and uncharged solutes, rapid gradient capability, direct on-column injection of physiological fluids, unique separation selectivity, high reproducibility, robustness, enhanced luminescence detection, low cost, and safety. This review is devoted to the evaluation of the agreement of MLC with the principles of green chemistry which recently represents a universal trend. Also, it provides an overview on the basics of MLC, in addition to a survey of MLC methods published in the past five years for the assay of various compounds in different matrices.

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“…Selection of the pH in the mobile phase is also often extremely important for the resolution of complex mixtures, owing to the side acid-base reactions of many solutes. Other variables to be considered are temperature and ionic strength [1].…”

Section: Introductionmentioning

confidence: 99%

Retention Behaviour in Micellar Liquid Chromatography

Rambla-Alegre

2012

Chromatography Research International

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Retention in micellar liquid chromatography is highly reproducible and can be modelled using empirical or mechanistic models with great accuracy to predict the retention changes when the mobile phase composition varies (surfactant and organic solvent concentrations), thus facilitating the optimisation of separation conditions. In addition, the different equilibria inside the column among the solute, the mobile phase, and the modified stationary phase by monomers of surfactant have been exhaustively studied. In a sequential strategy, the retention of the solutes is not known a priori, and each set of mobile phases is designed by taking into account the retention observed with previous eluents. By contrast, in an interpretative strategy, the experiments are designed before the optimization process and used to fit a model that will allow the prediction of the retention of each solute. This strategy is more efficient and reliable. The sequential strategy will be inadequate when several local and/or secondary maxima exist, as frequently occurs in chromatography, and may not give the best maximum, that is to say, the optimum. More often than not, the complexity of the mixtures of compounds studied and the relevant modification of their chromatographic behaviour when changing the mobile phase composition requires the use of computer-assisted simulations in MLC to follow the modifications in the chromatograms in detail. These simulations can be done with sound reliability thanks to the use of chemometrics tools.

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Retention mechanisms in micellar liquid chromatography

Cited by 147 publications

References 64 publications

Incorporating physiologically relevant mobile phases in micellar liquid chromatography for the prediction of human intestinal absorption

Incorporating physiologically relevant mobile phases in micellar liquid chromatography for the prediction of human intestinal absorption

Micellar Liquid Chromatography from Green Analysis Perspective

Retention Behaviour in Micellar Liquid Chromatography

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