Retention mechanism of pH‐peak‐focusing in countercurrent chromatographic separation of baicalin and wogonoside from <i>Scutellaria baicalensis</i> Georgi

Wang, Chaoyue; Zuo, Guanglei; Wang, Xiang; Kim, Hyun Yong; Zhao, Shanshan; Sun, Wenyu; Tong, Shengqiang; Lim, Soon Sung

doi:10.1002/jssc.202000455

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Furthermore, the excessive use of organic solvents is harmful to the environment. However, as a liquid–liquid partition chromatography, high-speed countercurrent chromatography (HSCCC) may use a liquid stationary phase without a solid support, thereby avoiding irreversible adsorption and benefiting complete sample recovery [ 59 , 60 ]. HSCCC has been successfully used to separate the antitumor compound antroquinonol from the solid-state cultured mycelia of A .…”

Section: Isolation and Purification Of Bioactive Compounds From ...mentioning

confidence: 99%

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Wang

et al. 2022

Bioengineering

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Antrodia cinnamomea is a precious and popular edible and medicinal mushroom. It has attracted increasing attention due to its various and excellent bioactivities, such as hepatoprotection, hypoglycemic, antioxidant, antitumor, anticancer, anti-inflammatory, immunomodulation, and gut microbiota regulation properties. To elucidate its bioactivities and develop novel functional foods or medicines, numerous studies have focused on the isolation and identification of the bioactive compounds of A. cinnamomea. In this review, the recent advances in bioactivity, isolation, purification, and identification methods of active compounds from A. cinnamomea were summarized. The present work is beneficial to the further isolation and discovery of new active compounds from A. cinnamomea.

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Section: Isolation and Purification Of Bioactive Compounds From ...mentioning

confidence: 99%

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Wang

et al. 2022

Bioengineering

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“…However, compounds 1 and 3 exhibited a small α value (α K1/K3 = 1.29) using n-hexane/EtOAc/ MeOH/water (2:5:2:5, v/v). Subsequently, modification of the solvent system by adding acid, a widely used strategy for HSCCC separation [22,25], was performed by adding acetic acid (0.1%, v/v) to the solvent system n-hexane/EtOAc/MeOH/water (2:5:2:5, v/v). However, this did not improve the α value of compounds 1 and 3 (α K1/K3 = 1.27).…”

Section: Antioxidant Ar Inhibition and Hplc Profile Of The Extracts A...mentioning

confidence: 99%

Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling

Zuo

Quispe

et al. 2021

Plants

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We previously reported that Lepechinia meyenii (Walp.) Epling has antioxidant and aldose reductase (AR) inhibitory activities. In this study, L. meyenii was extracted in a 50% MeOH and CH2Cl2/MeOH system. The active extracts of MeOH and 50% MeOH were subjected to fractionation, followed by separation using high-speed counter-current chromatography (HSCCC) and preparative HPLC. Separation and identification revealed the presence of caffeic acid, hesperidin, rosmarinic acid, diosmin, methyl rosmarinate, diosmetin, and butyl rosmarinate. Of these, rosmarinic acid, methyl rosmarinate, and butyl rosmarinate possessed remarkable antioxidant and AR inhibitory activities. The other compounds were less active. In particular, rosmarinic acid is the key contributor to the antioxidant and AR inhibitory activities of L. meyenii; it is rich in the MeOH extract (333.84 mg/g) and 50% MeOH extract (135.41 mg/g) of L. meyenii and is especially abundant in the EtOAc and n-BuOH fractions (373.71–804.07 mg/g) of the MeOH and 50% MeOH extracts. The results clarified the basis of antioxidant and AR inhibitory activity of L. meyenii, adding scientific evidence supporting its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study can be used for the preparative separation of rosmarinic acid from natural products.

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“…However, high‐speed countercurrent chromatography (HSCCC) can achieve this goal. As a liquid–liquid partition chromatography, HSCCC adopts a liquid stationary phase (SP) without solid support, which avoids irreversible adsorption and total sample recovery [14, 15]. In addition, load capacity of HSCCC is large.…”

Section: Introductionmentioning

confidence: 99%

An efficient high‐speed countercurrent chromatography method for preparative isolation of highly potent anti‐cancer compound antroquinonol from Antrodia camphorata after experimental design optimized extraction

Tao

Chen

Pu³

et al. 2021

J of Separation Science

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To avoid irreversible stationary phase adsorption and tedious and time‐consuming separation steps, high‐speed countercurrent chromatography was employed for the preparative separation of anti‐tumor compound antroquinonol from solid fermentation culture of Antrodia camphorata for the first time. A Box–Behnken experimental design, based on three parameters including liquid‐to‐solid ratio, extraction time, and extraction temperature, was applied to optimize the ultrasonic extraction procedure. The optimal extraction condition was set as follows: liquid‐to‐solid ratio: 49.57:1; extraction time: 55.76 min; extraction temperature was arranged as 44.21°C. Meanwhile, an optimized solvent system containing petroleum ether, ethyl acetate, methanol, and water (4:1:4:1, v/v/v/v) was selected for the preparative separation of antroquinonol at a flow rate of 2.0 mL/min. The yield of isolated antroquinonol was determined to be 6.0 mg from 0.67 g of ethyl acetate extracts. The isolated antroquinonol was elucidated by ultra‐high‐performance liquid chromatography–tandem mass spectrometry, and NMR spectroscopy, and by comparison with literature data. The purity of isolated antroquinonol was determined to be 97.12%. This study confirmed that high‐speed countercurrent chromatography was powerful and cost‐effective for the preparative separation of the high‐potently anti‐tumor compound antroquinonol from solid fermentation culture of A. camphorata.

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Retention mechanism of pH‐peak‐focusing in countercurrent chromatographic separation of baicalin and wogonoside from Scutellaria baicalensis Georgi

Cited by 5 publications

References 33 publications

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling

An efficient high‐speed countercurrent chromatography method for preparative isolation of highly potent anti‐cancer compound antroquinonol from Antrodia camphorata after experimental design optimized extraction

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