An efficient high‐speed countercurrent chromatography method for preparative isolation of highly potent anti‐cancer compound antroquinonol from <i>Antrodia camphorata</i> after experimental design optimized extraction

Tao, Yi; Chen, Lin; Pu, Faxiang; Xie, Zhangfu; Tong, Shengqiang; Yan, Jizhong

doi:10.1002/jssc.202100162

Cited by 11 publications

(10 citation statements)

References 16 publications

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“…cinnamomea , and the purity of the isolated antroquinonol was 97.12%. This method can avoid irreversible stationary-phase adsorption and tedious and time-consuming separation steps [ 61 ].…”

Section: Isolation and Purification Of Bioactive Compounds From ...mentioning

confidence: 99%

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Wang

et al. 2022

Bioengineering

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Antrodia cinnamomea is a precious and popular edible and medicinal mushroom. It has attracted increasing attention due to its various and excellent bioactivities, such as hepatoprotection, hypoglycemic, antioxidant, antitumor, anticancer, anti-inflammatory, immunomodulation, and gut microbiota regulation properties. To elucidate its bioactivities and develop novel functional foods or medicines, numerous studies have focused on the isolation and identification of the bioactive compounds of A. cinnamomea. In this review, the recent advances in bioactivity, isolation, purification, and identification methods of active compounds from A. cinnamomea were summarized. The present work is beneficial to the further isolation and discovery of new active compounds from A. cinnamomea.

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Section: Isolation and Purification Of Bioactive Compounds From ...mentioning

confidence: 99%

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Wang

et al. 2022

Bioengineering

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“…Tao et al. [109] successfully isolated highly potent anticancer compound antroquinonol from Antrodia camphorata using an HSCCC method. A BBD was applied to evaluate the changes of liquid‐to‐solid ratio, extraction time, and extraction temperature, which markedly affect the isolation yield of antroquinonol.…”

Section: Applications Of Doe In the Chromatographic Analysis Conditio...mentioning

confidence: 99%

“…The critical extraction parameters such as the concentration of chiral selector, pH value of the aqueous phase, and separation temperature were investigated through a BBD to obtain the objective of enantioseparation factors more than 1.30. Tao et al [109] successfully isolated highly potent anticancer compound antroquinonol from Antrodia camphorata using an HSCCC method. A BBD was applied to evaluate the changes of liquid-to-solid ratio, extraction time, and extraction temperature, which markedly affect the isolation yield of antroquinonol.…”

Section: High-speed Countercurrent Chromatography (Hsccc)mentioning

confidence: 99%

Design of experiment techniques for the optimization of chromatographic analysis conditions: A review

et al. 2022

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Design of experiment (DoE) techniques have been widely used in the field of chromatographic parameters optimization as a valuable tool. A systematic literature review of the available DoE techniques applied to the development of a chromatographic analysis method is presented in this paper. First, the most common available designs and the implementation steps of DoE are comprehensively introduced. Then the studies in recent 10 years for the application of DoE techniques in various chromatographic techniques are discussed, such as capillary electrophoresis, liquid chromatography, gas chromatography, thin‐layer chromatography, and high‐speed countercurrent chromatography. Current problems and future outlooks are finally given to provide a certain inspiration of research in the application of DoE techniques to the different chromatographic techniques field. This review contributes to a better understanding of the DoE techniques for the efficient optimization of chromatographic analysis conditions, especially for the analysis of complex systems, such as multicomponent drugs and natural products.

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“…Overlap of peaks will happen at a high sample loading amount which further limits the large scale separation. High-speed counter-current chromatography (HSCCC) is a fast-growing separation technique in natural products based on the liquid-liquid partition principle and has been reported for preparative separation and purification of various natural products [16][17][18][19][20]. In the most recent research, Wang et al used high-speed counter-current chromatography (HSCCC) followed by medium-pressure liquid chromatography to isolate monoterpene glycosides [21], which was able to achieve a high recovery yield of 95%.…”

Section: Introductionmentioning

confidence: 99%

“…Overlap of peaks will happen at a high sample loading amount which further limits the large scale separation. High‐speed counter‐current chromatography (HSCCC) is a fast‐growing separation technique in natural products based on the liquid‐liquid partition principle and has been reported for preparative separation and purification of various natural products [16–20]. In the most recent research, Wang et al.…”

Section: Introductionmentioning

confidence: 99%

An efficient high‐speed counter‐current chromatography method for the preparative separation of potential antioxidants from Paeonia lactiflora Pall. combination of in vitro evaluation and molecular docking

Sun

Chen

Yan

et al. 2022

J of Separation Science

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Paeonia lactiflora Pall., one of the most famous classical herbal medicine, has been used to treat diseases for over 1200 years. In this research, the functional ingredients were purified by online‐switch two‐dimensional high‐speed counter‐current chromatography combined with inner‐recycling and continuous injection mode. The antioxidant activity was evaluated by investigating the 2,2′‐azobis (2‐amidinopropane) dihydrochloride‐induced oxidant damage in vitro and confirmed through molecular docking. n‐Butanol/ethyl acetate/water (2:3:5, v/v) solvent system was used for the first‐dimensional separation and optimized the sample loading. Two pure compounds and a polyphenol‐enriched fraction were separated. The polyphenol‐enriched fraction was separated with a solvent system n‐hexane/ethyl acetate/methanol/water (2:8:4:6, v/v) with continuous injection mode. Five compounds were successfully separated, including gallic acid (1), methyl gallate (2), albiflorin (3), paeoniflorin (4), and ethyl gallate (5). Their structures were identified by mass spectrometry and NMR spectroscopy. The results from the antioxidant effect showed that albiflorin had stronger antioxidant activity. Molecular docking results indicated that the affinity energy of the identified compounds ranged from ‐3.79 to ‐8.22 kcal/mol and albiflorin showed the lowest affinity energy. Overall, all those findings suggested that the strong antioxidant capacity of albiflorin can be potentially used for the treatment of diseases caused by oxidation.

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An efficient high‐speed countercurrent chromatography method for preparative isolation of highly potent anti‐cancer compound antroquinonol from Antrodia camphorata after experimental design optimized extraction

Cited by 11 publications

References 16 publications

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Review of Bioactivity, Isolation, and Identification of Active Compounds from Antrodia cinnamomea

Design of experiment techniques for the optimization of chromatographic analysis conditions: A review

An efficient high‐speed counter‐current chromatography method for the preparative separation of potential antioxidants from Paeonia lactiflora Pall. combination of in vitro evaluation and molecular docking

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