Retention and Separation Selectivity of Natural Phenolic Antioxidants on Zirconia Based Stationary Phases

Beldean‐Galea, Mihail Simion; Jandera, Pavel; Hodisan, Sorin

doi:10.1080/10826070801890454

Cited by 5 publications

(5 citation statements)

References 34 publications

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“…Because of their excellent thermal and chemical stability, zirconia (Zr)‐based columns have also been investigated in the HTLC analysis of phenolic compounds 81. Although these columns were found to offer alternative selectivity compared with silica‐based columns, most flavone antioxidants were too strongly retained on the Zr‐carbon and Zr‐carbon C18 columns.…”

Section: Applications Of Recent Advances In Hplc To Phenolic Analysismentioning

confidence: 99%

Recent developments in the HPLC separation of phenolic compounds

Kalili

Villiers

2011

J of Separation Science

117

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Phenolic compounds represent a class of highly complex naturally occurring molecules that possess a range of beneficial health properties. As a result, considerable attention has been devoted to the analysis of phenolics in a variety of samples. HPLC is the workhorse method for phenolic separation. However, conventional HPLC methods provide insufficient resolving power when faced with the complexity of real-world phenolic fractions. This limitation has been traditionally circumvented by extensive sample fractionation, multiple analysis methods and/or selective detection strategies. On the other hand, there is an increasing demand for improved throughput and resolving power from the chromatographic methods used for phenolic analyses. Fortunately, during the last decade, a number of important technological advances in LC have demonstrated significant gains in terms of both speed and resolution. These include ultra high-pressure liquid chromatography (UHPLC), high-temperature liquid chromatography (HTLC), multi-dimensional separations as well as various new stationary phase chemistries and morphologies. In recent years, these technologies have also found increasing application for phenolic analysis. This review seeks to provide an updated overview of the application of recent advances in HPLC to phenolic separation, with the emphasis on how these methodologies can contribute to improve performance in HPLC analysis of phenolics.

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Section: Applications Of Recent Advances In Hplc To Phenolic Analysismentioning

confidence: 99%

Recent developments in the HPLC separation of phenolic compounds

Kalili

Villiers

2011

J of Separation Science

117

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“…48,50 A zircônia é o óxido metálico mais estudado e difundido por apresentar maior estabilidade química e térmica, na faixa de pH de 1 a 14 e em temperaturas de até 200 °C, respectivamente, e, também, alta resistência mecânica. 13,51,52 A coluna zirconizada mais popular disponível comercialmente é a ZirChrom ® -PBD (zircônia recoberta com polibutadieno) da ZirChrom ® . 47,49,53 A titânia tende a ter propriedades e mecanismos de retenção similares à zircônia.…”

Section: Figuraunclassified

Fases estacionárias modernas para cromatografia líquida de alta eficiência em fase reversa

Maldaner¹,

Jardim²

2010

Quím. Nova

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Recebido em 15/12/09; aceito em 1/4/10; publicado na web em 29/6/10 MODERN STATIONARY PHASES FOR REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. This review first discusses the limitations of many of the supports and stationary phases used in reversed phase high performance liquid chromatography and then describes those, developed more recently, that present better stabilities and more versatile selectivities. Emphases will be given to stationary phases that use higher purity silicas, hybrid silicas, monolithic silicas, metallic oxides and mixed oxides as supports and those that have embedded polar groups or contain phenyl or fluoro groups as the stationary phase as well as the phases used for mixed mode or hydrophilic interaction separations. These modern stationary phases facilitate the analysis of complex mixtures.Keywords: RP-HPLC; modern stationary phases; stability. INTRODUÇÃOO alto nível de desenvolvimento e expansão alcançado pela cromatografia líquida de alta eficiência em fase reversa (CLAE-FR), ao longo das últimas décadas, deve-se principalmente a sua vasta aplicabilidade, que engloba o desenvolvimento de novos produtos, o controle da qualidade desses produtos e, também, as possíveis contaminações provenientes desses produtos e dos disponíveis no mercado há anos. Dentro deste contexto estão envolvidas as indústrias farmacêuticas, alimentícias, agropecuárias e químicas, as agências reguladoras que tratam do meio ambiente e outras. A ampla aplicabilidade da CLAE-FR foi o que impulsionou e continua impulsionando as pesquisas em busca do aprimoramento desta técnica, principalmente referente ao desenvolvimento de novas fases estacionárias (FE). Hoje a CLAE-FR pode ser considerada uma técnica de análise (separação, confirmação e quantificação) bem difundida e empregada, em consequência das colunas cromatográficas e equipamentos de alta tecnologia que se encontram disponíveis.As FE empregadas em CLAE-FR consistem de uma camada orgânica apolar ligada quimicamente ou imobilizada ou apenas sorvida a um suporte cromatográfico. A forma de obtenção dessas camadas apolares e a variedade de óxidos empregados como suportes cromatográficos resultam nos diferentes tipos de FE. O método mais comum e também o mais empregado para a obtenção das FE consiste na introdução de monocamadas orgânicas através de reações com reagentes apropriados, que dá origem às fases estacionárias quimicamente ligadas (FEQL).1-4 As FEQL podem ser obtidas por diferentes processos, como esterificação, cloração seguida por reação com reagente de Grignard ou com compostos organo-lítio e organossilanização. Uma forma alternativa de preparo de FE é o método de recobrimento do suporte com polímeros orgânicos, que pode ser realizado de duas maneiras: mistura de um polímero pré-sintetizado de composição química bem definida em solução com o suporte e posterior evaporação do solvente e, polimerização in situ de uma solução de monômeros ou oligômeros, iniciada ou propagada na superfície do suporte, geralmente seguida de uma etapa de imobilização....

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“…Simultaneous determination of microbial‐derived aromatic acids in biological specimens is challenging. So far, numerous analytical methods have been developed to quantify the microbial‐derived metabolites in various biological fluids, including LC coupled with the detection of ultraviolet (Beldean‐Galea, Jandera, & Hodisan, 2008), diode array (Gonthier et al, 2003a; Nováková, Spáčil, Seifrtová, Opletal, & Solich, 2010), or tandem mass spectrometry (MS/MS; Gasperotti, Masuero, Guella, Mattivi, & Vrhovsek, 2014; Gonthier, Rios, Verny, Rémésy, & Scalbert, 2003b; Margalef, Pons, Muguerza, & Arola‐Arnal, 2014); and GC–mass spectrometry (GC–MS) (Liu, Kushnir, Roberts, & Pasquali, 2004; Ordóñez et al, 2018; Paprotny, Celejewska, Frajberg, & Wianowska, 2019). Traditionally, LC–ultraviolet and LC–diode array show features of weak sensitivity and poor selectivity, which restrict their use in detecting low‐concentration metabolites (Nováková et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study

Zou

Sun

et al. 2020

Biomedical Chromatography

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Gut microbial phenylalanine, tyrosine, and tryptophan metabolites are closely linked to various diseases. Monitoring the alterations of the related metabolites is vital to facilitate the understanding of pathophysiology of diseases. Herein, a rapid and sensitive assay based on LC–tandem mass spectrometry has been developed to analyze 20 gut microbial metabolites derived from phenylalanine, tyrosine, and tryptophan in rat serum, urine, and faeces. These microbial‐derived metabolites were separated on a phenyl‐hexyl column and simultaneously determined in a single run of 8 min. The detection limit for analytes ranged between 1.08 and 32.4 ng/mL. All calibration curves exhibited good linear relationships (R2 ≥ 0.9982). Intra‐ and inter‐assay precision values were below 15% and accuracies ranged from 85% to 115% for all analytes. The selectivity, matrix effect, and recovery of this method were all satisfactory. The validated method was successfully applied to characterize the alterations of these metabolites in type 2 diabetes mellitus rat. In general, the developed assay is suitable for high‐throughput monitoring of gut microbial phenylalanine, tyrosine, and tryptophan metabolites and provides a useful approach for exploring the mechanisms of microbial‐derived metabolites in diseases.

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Retention and Separation Selectivity of Natural Phenolic Antioxidants on Zirconia Based Stationary Phases

Cited by 5 publications

References 34 publications

Recent developments in the HPLC separation of phenolic compounds

Recent developments in the HPLC separation of phenolic compounds

Fases estacionárias modernas para cromatografia líquida de alta eficiência em fase reversa

Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study

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