Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting. The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosanâAPP films (CAâfilm) was investigated. Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP. The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images. Xâray diffraction (XRD) patterns suggest the semiâcrystalline structure was increased upon addition of APP. The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd) and water vapor permeability (WVP) compared with chitosan film (Câfilm), respectively. However, the transparency decreased slightly, which may be due to the shrinkage of the spacing of the polymer interchain. The composite films also displayed enhanced the mechanical properties. The antimicrobial activity of the CAâfilm showed an increase of at least of 0.41âfold in inhibition zone diameter for E. coli. At a concentration of 1.2Â mg/mL, the antioxidant activity of CAâfilm was enhanced by more than threefold compared with Câfilm. Therefore, CAâfilms have good potential as sources of active packaging material for the food industry.