Retardation of some drugs in thin-layer chromatographic systems with impregnated silica gel plates with hen's egg white and bovine serum albumin

Jaglińska, Kamila; Polak, Beata; Klimek-Turek, Anna; Pomastowski, Paweł; Dzido, Tadeusz H.

doi:10.1016/j.chroma.2020.461277

Cited by 7 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 15 × 33 cm horizontal chamber prototype was used (Department of Physical Chemistry, Medical University, Lublin, Poland) for this process. Other details of the impregnation process were presented in our previous paper [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous article, we focused only on the effect of albumin sorbent impregnation hen’s (egg white and bovine serum albumin) on the drug sample preparation process [ 18 ]. It turned out that isolation of substances from a biological matrix is more complicated and requires additional development of chromatograms to obtain the investigated substances in the front position of the mobile phase [ 18 ]. Hence, the purpose of this paper is to check the suitability of SFPE for the preparation of biological samples with antihypertensive drugs before their further quantitative determinations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of Antihypertensive Drugs in Biological Matrix with Solvent Front Position Extraction for LC–MS/MS Analysis

et al. 2021

Self Cite

View full text Add to dashboard Cite

Solvent front position extraction procedure was used to prepare biological samples containing selected antihypertensive drugs (ramipril, lercanidipine, indapamide, valsartan, hydrochlorothiazide, perindopril, and nebivolol). Substances separated from the biological matrix components (bovine serum albumin) were quantified by means of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Sample preparation process was performed with the use of a prototype horizontal chamber with a moving pipette driven by a 3D printer mechanism enabling a controlled eluent flow velocity. Application of this device was advantageous for simultaneous preparation of several samples for further quantitative analysis, with a synchronized reduction of manual operations and solvent consumption. Quantitative results obtained for the majority of the investigated antihypertensive drugs in a complex biological matrix were satisfactory. The values of the %RSD were around 5% for six of the seven substances (with the exception of indapamide). The method exhibits a suitable accuracy (the relative error percentage was below 10% for most drugs). The values of LOD and LOQ were in the range of 1.19 µg/L–8.53 µg/L and 3.61 µg/L–25.8 µg/L, respectively.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of Antihypertensive Drugs in Biological Matrix with Solvent Front Position Extraction for LC–MS/MS Analysis

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thanks to its biocompatibility, metabolic activity, non-antigenicity, and availability, BSA has gained increasing attention in biotechnology and nanomedicine 51 . As an ampholyte protein (containing both acidic and basic residues), it can bind both cations and anions 52 . By possessing 582 amino acid residues and various functional groups, especially thiols group, BSA is suitable for adsorbing metal ions from water 29 , 52 .…”

Section: Protein Nanofibrils As New Water Purification Agentsmentioning

confidence: 99%

“…As an ampholyte protein (containing both acidic and basic residues), it can bind both cations and anions 52 . By possessing 582 amino acid residues and various functional groups, especially thiols group, BSA is suitable for adsorbing metal ions from water 29 , 52 .…”

Section: Protein Nanofibrils As New Water Purification Agentsmentioning

confidence: 99%

Protein nanofibrils for next generation sustainable water purification

2021

View full text Add to dashboard Cite

Water scarcity is rapidly spreading across the planet, threatening the population across the five continents and calling for global sustainable solutions. Water reclamation is the most ecological approach for supplying clean drinking water. However, current water purification technologies are seldom sustainable, due to high-energy consumption and negative environmental footprint. Here, we review the cutting-edge technologies based on protein nanofibrils as water purification agents and we highlight the benefits of this green, efficient and affordable solution to alleviate the global water crisis. We discuss the different protein nanofibrils agents available and analyze them in terms of performance, range of applicability and sustainability. We underline the unique opportunity of designing protein nanofibrils for efficient water purification starting from food waste, as well as cattle, agricultural or dairy industry byproducts, allowing simultaneous environmental, economic and social benefits and we present a case analysis, including a detailed life cycle assessment, to establish their sustainable footprint against other common natural-based adsorbents, anticipating a bright future for this water purification approach.

show abstract

“…Protein molecules (M.m. 64-69 kiloDa) are a globule in the form of an oblate ellipsoid of revolution, with crystallographic dimensions (8)(9)(10)(11)(12)(13) nm × (4-5) nm (from the DOI database: 10.2210/pdb4F5S/pdb). The BSA molecule has an equal number of hydrophobic and hydrophilic amino acid residues; isoelectric point (pJ) is 4.8-5.6, i.e., the BSA molecule is neutral in distilled water with a pH~6.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Aggregation of Nanoparticles on Aluminum and Gold Surfaces Occurring in Bovine Serum Albumin Solutions

Саланов

Серкова

Zhirnova

et al. 2022

Micro

View full text Add to dashboard Cite

The supramolecular aggregation processes occurring on metallic (aluminum and gold) surfaces in aqueous solutions of bovine serum albumin (BSA) during drying were studied using advanced scanning electron microscopy (SEM). The possible mechanism for the formation of amazing intricate fractal structures on metallic surfaces was proposed based on the analysis of SEM images, size distribution diagrams and EDX-scanning element distribution maps.

show abstract

Retardation of some drugs in thin-layer chromatographic systems with impregnated silica gel plates with hen's egg white and bovine serum albumin

Cited by 7 publications

References 28 publications

Preparation of Antihypertensive Drugs in Biological Matrix with Solvent Front Position Extraction for LC–MS/MS Analysis

Preparation of Antihypertensive Drugs in Biological Matrix with Solvent Front Position Extraction for LC–MS/MS Analysis

Protein nanofibrils for next generation sustainable water purification

Supramolecular Aggregation of Nanoparticles on Aluminum and Gold Surfaces Occurring in Bovine Serum Albumin Solutions

Contact Info

Product

Resources

About