Preparation of Antihypertensive Drugs in Biological Matrix with Solvent Front Position Extraction for LC–MS/MS Analysis

Jaglińska, Kamila; Polak, Beata; Klimek-Turek, Anna; Błaszczyk, Robert; Wysokiński, Andrzej; Dzido, Tadeusz H.

doi:10.3390/molecules27010205

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Solvent front position extraction is a variation of pSPE in which the target compounds and an internal standard for quantification are in a single zone at the solvent front position of the final development [114][115][116][117][118]. This has the advantage that the target compounds are concentrated in a narrow zone with an easily defined location for subsequent solvent extraction.…”

Section: Planar Spementioning

confidence: 99%

“…Vial collection of the effluent from solvent‐extracted zones is a flexible option for off‐line coupling of HPTLC to column systems with the possibility of storing or splitting the extract for subsequent separation by different column systems, solvent exchange, or concentration [105–110, 116, 118, 123]. It offers a straightforward approach for heart‐cut multidimensional chromatography using HPTLC and any form of column chromatography (e.g., GC, HPLC, SFE) for the final separation with a wide range of detection options, some of which are not otherwise available for HPTLC.…”

Section: Solid‐phase Extractionmentioning

confidence: 99%

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Section: Planar Spementioning

confidence: 99%

Section: Solid‐phase Extractionmentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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Planar chromatography – Current practice and future prospects

Wilson

Poole

2023

Journal of Chromatography B

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Comparison of the Determination of Some Antihypertensive Drugs in Clinical Human Plasma Samples by Solvent Front Position Extraction and Precipitation Modes

et al. 2023

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The determination of the selected antihypertensive drugs in human plasma samples with the novel solvent front position extraction (SFPE) technique is presented. The SFPE procedure combined with LC-MS/MS analysis was used for the first time to prepare a clinical sample containing the drugs mentioned above from different therapeutic groups. The effectiveness of our approach was compared with the precipitation method. The latter technique is usually used to prepare biological samples in routine laboratories. During the experiments, the substances of interest and the internal standard were separated from other matrix components using a prototype horizontal chamber for thin-layer chromatography/high-performance thin-layer chromatography (TLC/HPTLC) with a moving pipette powered by a 3D mechanism, which distributed the solvent on the adsorbent layer. Detection of the six antihypertensive drugs was performed by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) in multiple reaction monitoring (MRM) mode. Results obtained by SFPE were very satisfactory (linearity R2 ≥ 0.981; %RSD ≤ 6%; LOD and LOQ were in the range of 0.06–9.78 ng/mL and 0.17–29.64 ng/mL, respectively). The recovery was in the range of 79.88–120.36%. Intra-day and inter-day precision had a percentage coefficient of variation (CV) in the range of 1.10–9.74%. The procedure is simple and highly effective. It includes the automation of TLC chromatogram development, which significantly reduced the number of manual operations performed, the time of sample preparation and solvent consumption.

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Preparation of Antihypertensive Drugs in Biological Matrix with Solvent Front Position Extraction for LC–MS/MS Analysis

Cited by 3 publications

References 25 publications

Sample preparation for planar chromatography

Sample preparation for planar chromatography

Planar chromatography – Current practice and future prospects

Comparison of the Determination of Some Antihypertensive Drugs in Clinical Human Plasma Samples by Solvent Front Position Extraction and Precipitation Modes

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