Resveratrol’s neural protective effects for the injured embryoid body and cerebral organoid

Abstract

Objective Resveratrol (RSV) is a polyphenol compound found in grapes, veratrum and other plants. It has been reported that RSV has anti-inflammatory, anti-oxidant, anti-cancer and other pharmacological effects. However, the impacts of RSV on development of nervous system are not understood well. The study aims to investigate RSV’s neuroprotective effect during development and to provide a health care for pregnant women and their fetuses with RSV supplementation. Methods In this study, we induced human induced pluripotent stem cells (hiPSCs) to form the embryoid bodies (EBs) and cerebral organoids (COs) with 3 dimensional (3D) culture. In the meantime, D-galactose (D-gal, 5 mg/ml) was used to make nervous injury model, and on the other hand, RSV with various doses, such as 2 μm/L, 10 μm/L, 50 μm/L, were applied to understand its neuroprotection. Therefore, the cultures were divided into control group, D-gal nervous injury group and RSV intervention groups. After that, the diameters of EBs and COs were measured regularly under a reverted microscope. In the meantime, the neural proliferation, cell apoptosis and the differentiation of germ layers were detected via immunofluorescence. Results (1) D-gal could delay the development of EBs and COs; (2) RSV could rescue the atrophy of EBs and COs caused by D-gal; (3) RSV showed its neuroprotection, through promoting the neural cell proliferation, inhibiting apoptosis and accelerating the differentiation of germ layers. Conclusion RSV has a neuroprotective effect on the development of the nervous system, suggesting RSV supplementation may be necessary during the health care of pregnancy and childhood.

Bioactivity and toxicity evaluation of nutraceuticals using in vitro cell-based models: A review

“…Wang et al have evaluated the neuroprotective mechanism of resveratrol using cerebral organoids 3D-cell culture. The result showed that resveratrol could protect the neuronal cell from damage caused by D-galactose by inducing neuronal cell proliferation, inhibiting apoptosis, and enhancing the differentiation of germ layers [57]. These results show the initial applications and potential of using 3D-cell culture models in the study of toxicity and bioactivity of nutraceutical compounds.…”

Cerebral Organoid Arrays for Batch Phenotypic Analysis in Sections and Three Dimensions

“…In addition to using patient-derived cerebral organoids, we could also treat cerebral organoids with certain drugs in vitro to mimic aging or damaged brains in humans. These induced disease models include the D-galactose-induced aging model [ 6 ], the hydrogen peroxide-induced oxidative stress model [ 7 ], the lipopolysaccharide (LPS)-induced inflammation model [ 8 ], the alcohol-induced neurotoxicity model [ 9 , 10 ], etc. These organoid models have contributed to our understanding of disease mechanisms and driven innovation for new therapies.…”