Resveratrol protects primary rat hepatocytes against oxidative stress damage:

Rubiolo, Juan A.; Mithieux, Gilles; Vega, Félix

doi:10.1016/j.ejphar.2008.06.067

Cited by 280 publications

(83 citation statements)

References 51 publications

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“…RVT did not reverse the effects of CrVI; rather, it enhanced SOD1 and 2 compared to control and CrVI. RVT increased GSH synthesis (Kode et al, 2008) and activated catalase, SOD, and NQO1 (Rubiolo et al, 2008) in rat hepatocytes. Overall, we speculate that RVT may have protected the follicles from CrVI-induced oxidative stress by increasing the transcription, translation and/or activity of endogenous AOX enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…It also plays several roles in anti-aging (Agarwal and Baur, 2011) and anti-inflammatory pathways (Yu et al, 2012), and ameliorating metabolic syndrome (Beaudeux et al, 2010). The diversified biological effects of RVT might be explained in part by RVT’s antioxidant (AOX) properties, including increases in catalase and superoxide dismutase (SOD) activities (Rubiolo et al, 2008). The AOX effect of RVT has been shown to be due to the presence of the phenolic hydroxyl groups in it’s chemical structure (Leonard et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol protects the ovary against chromium-toxicity by enhancing endogenous antioxidant enzymes and inhibiting metabolic clearance of estradiol

Banu

Stanley

Sivakumar

et al. 2016

Toxicology and Applied Pharmacology

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Resveratrol (RVT), a polyphenolic component in grapes and red wine, has been known for its cytoprotective actions against several diseases. However, beneficial effects of RVT against early exposure to endocrine disrupting chemicals (EDCs) have not been understood. EDCs are linked to several ovarian diseases such as premature ovarian failure, polycystic ovary syndrome, early menopause and infertility in women. Hexavalent chromium (CrVI) is a heavy metal EDC, and widely used in >50 industries. Environmental contamination with CrVI in the US is rapidly increasing, predisposing the human to several illnesses including cancers and still birth. Our lab has been involved in determining the molecular mechanism of CrVI-induced female infertility and intervention strategies to mitigate CrVI effects. Lactating mother rats were exposed to CrVI (50 ppm potassium dichromate) from postpartum days 1–21 through drinking water with or without RVT (10 mg/kg body wt., through oral gavage daily). During this time, F1 females received respective treatments through mother’s milk. On postnatal day (PND) 25, blood and the ovary, kidney and liver were collected from the F1 females for analyses. CrVI increased atresia of follicles by increasing cytochrome C and cleaved caspase-3; decreasing antiapoptotic proteins; decreasing estradiol (E2) biosynthesis and enhancing metabolic clearance of E2, increasing oxidative stress and decreasing endogenous antioxidants. RVT mitigated the effects of CrVI by upregulating cell survival proteins and AOXs; and restored E2 levels by inhibiting hydroxylation, glucuronidation and sulphation of E2. This is the first study to report the protective effects of RVT against any toxicant in the ovary.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Resveratrol protects the ovary against chromium-toxicity by enhancing endogenous antioxidant enzymes and inhibiting metabolic clearance of estradiol

Banu

Stanley

Sivakumar

et al. 2016

Toxicology and Applied Pharmacology

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“…Chung et al [22] reported that green tea extract inhibits ROS/RNS-mediated damage while reducing serum alanine aminotransferase (ALT) and decreasing lipid peroxidation and protein nitration. Rubiolo et al [25] similarly revealed that resveratrol protects isolated primary rat hepatocytes from necrosis induced by oxidative stress. Gómez-Zorita et al [27] demonstrated that a 6-week treatment of resveratrol significantly decreased lipid peroxidation, suggesting a protective antioxidant effect against obesity and steatosis-induced oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Herbal adaptogens combined with protein fractions from bovine colostrum and hen egg yolk reduce liver TNF-α expression and protein carbonylation in Western diet feeding in rats

Mobley

Toedebusch

Cm³

et al. 2014

Nutr Metab (Lond)

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BackgroundWe examined if a purported anti-inflammatory supplement (AF) abrogated Western-diet (WD)-induced liver pathology in rats. AF contained: 1) protein concentrates from bovine colostrum and avian egg yolk; 2) herbal adaptogens and antioxidants; and 3) acetyl-L-carnitine.MethodsNine month-old male Brown Norway rats were allowed ad libitum access to WD for 41–43 days and randomly assigned to WD + AF feeding twice daily for the last 31–33 days (n = 8), or WD and water-placebo feeding twice daily for the last 31–33 days (n = 8). Rats fed a low-fat/low-sucrose diet (CTL, n = 6) for 41–43 days and administered a water-placebo twice daily for the last 31–33 days were also studied. Twenty-four hours following the last gavage-feed, liver samples were analyzed for: a) select mRNAs (via RT-PCR) as well as genome-wide mRNA expression patterns (via RNA-seq); b) lipid deposition; and, c) protein carbonyl and total antioxidant capacity (TAC). Serum was also examined for TAC, 8-isoprostane and clinical chemistry markers.ResultsWD + AF rats experienced a reduction in liver Tnf-α mRNA (-2.8-fold, p < 0.01). Serum and liver TAC was lower in WD + AF versus WD and CTL rats (p < 0.05), likely due to exogenous antioxidant ingredients provided through AF as evidenced by a tendency for mitochondrial SOD2 mRNA to increase in WD + AF versus CTL rats (p = 0.07). Liver fat deposition nor liver protein carbonyl content differed between WD + AF versus WD rats, although liver protein carbonyls tended to be lower in WD + AF versus CTL rats (p = 0.08). RNA-seq revealed that 19 liver mRNAs differed between WD + AF versus WD when both groups were compared with CTL rats (+/- 1.5-fold, p < 0.01). Bioinformatics suggest that AF prevented WD-induced alterations in select genes related to the transport and metabolism of carbohydrates in favor of select genes related to lipid transport and metabolism. Finally, serum clinical safety markers and liver pathology (via lesion counting) suggests that chronic consumption of AF was well tolerated.ConclusionsAF supplementation elicits select metabolic, anti-inflammatory, and anti-oxidant properties which was in spite of WD feeding and persisted up to 24 hours after receiving a final dose.

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“…Less signifi cant changes were observed in the content of mRNA for CYP1A1, CYP1A2, and CYP3A1. In vitro experiments showed that RES increases the expression of the Nrf2 gene and activities of quinone reductase, glutathione transferase, and some antioxidant enzymes [14]. In our experiments, RES had no effect on activity of these enzymes (including Nrf2-regulated enzyme hemoxygenase-1).…”

Section: Resultsmentioning

confidence: 59%

“…Similarly to α-tocopherol, RES inhibits lipid peroxidation (LPO) due to trapping of lipid peroxide radicals [8]. Apart from antiradical effect, RES increases activity of factor Nrf2 and antioxidant and cytoprotective enzymes in various cells [14]. The so-called "French paradox" (relatively low incidence of cardiovascular disease in the Mediterranean population) is associated with high consumption of RES.…”

mentioning

confidence: 99%

Effects of Combined Treatment with Resveratrol and Indole-3-Carbinol

et al. 2010

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Male Wistar rats received a semisynthetic diet with resveratrol (100 mg/kg), indole-3-carbinol (20 mg/kg), or a mixture of these compounds in the same doses for 1 week. Activities of ethoxyresorufin dealkylase (EROD), methoxyresorufin dealkylase (MROD), pentoxyresorufin dealkylase (PROD), and 6β-testosterone hydroxylase (6β-TH) and the content of mRNA for CYP1A1, CYP1A2, and CYP3A1 were elevated in the liver of rats receiving indole-3-carbinol. These changes were accompanied by an increase in activity of phase II xenobiotic metabolism enzymes (quinone reductase, hemoxygenase-1, glutathione transferase, and UDP glucuronosyl transferase). Resveratrol did not modify activity of these enzymes. After combined treatment with the test compounds, resveratrol suppressed the indole-3-carbinol-induced increase in activities of EROD, MROD, PROD, and 6β-TH, and expression of the corresponding genes. Combined treatment was characterized by potentiation of the antioxidant effects of these compounds.

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Resveratrol protects primary rat hepatocytes against oxidative stress damage:

Cited by 280 publications

References 51 publications

Resveratrol protects the ovary against chromium-toxicity by enhancing endogenous antioxidant enzymes and inhibiting metabolic clearance of estradiol

Resveratrol protects the ovary against chromium-toxicity by enhancing endogenous antioxidant enzymes and inhibiting metabolic clearance of estradiol

Herbal adaptogens combined with protein fractions from bovine colostrum and hen egg yolk reduce liver TNF-α expression and protein carbonylation in Western diet feeding in rats

Effects of Combined Treatment with Resveratrol and Indole-3-Carbinol

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