Effects of Combined Treatment with Resveratrol and Indole-3-Carbinol

Abstract

Male Wistar rats received a semisynthetic diet with resveratrol (100 mg/kg), indole-3-carbinol (20 mg/kg), or a mixture of these compounds in the same doses for 1 week. Activities of ethoxyresorufin dealkylase (EROD), methoxyresorufin dealkylase (MROD), pentoxyresorufin dealkylase (PROD), and 6β-testosterone hydroxylase (6β-TH) and the content of mRNA for CYP1A1, CYP1A2, and CYP3A1 were elevated in the liver of rats receiving indole-3-carbinol. These changes were accompanied by an increase in activity of phase II xenobiotic metabolism enzymes (quinone reductase, hemoxygenase-1, glutathione transferase, and UDP glucuronosyl transferase). Resveratrol did not modify activity of these enzymes. After combined treatment with the test compounds, resveratrol suppressed the indole-3-carbinol-induced increase in activities of EROD, MROD, PROD, and 6β-TH, and expression of the corresponding genes. Combined treatment was characterized by potentiation of the antioxidant effects of these compounds.

Indole-3-carbinol protects against cisplatin-induced acute nephrotoxicity: role of calcitonin gene-related peptide and insulin-like growth factor-1

“…Indole-3-carbinol was purchased from Sigma Chemical Co. (St. Louis, MO, USA). The dose used was selected according to previous studies 16 17 . Besides, pilot experimental trials of the present study were carried out for the same purpose.…”

“…Indole-3-carbinol is a natural glucosinolate breakdown product found in cruciferous vegetables 15 . It was shown that indole-3-carbinol has the ability to increase the activity of some anti-oxidant enzymes such as hemoxygenase-1 and glutathione transferase 16 . Besides, several studies had confirmed the anti-inflammatory activities of indole-3-carbinol in a variety of animal models such as aspirin-induced gastric ulcer 17 , liver fibrosis 18 , lipopolysaccharide-induced acute lung injury 19 and high fat diet-induced obesity 20 .…”