Resveratrol protects intestinal integrity, alleviates intestinal inflammation and oxidative stress by modulating AhR/Nrf2 pathways in weaned piglets challenged with diquat

Xun, Wenjuan; Fu, Qingyao; Shi, Liguang; Cao, Ting; Jiang, Hongzheng; Ma, Zhonghua

doi:10.1016/j.intimp.2021.107989

Cited by 37 publications

(33 citation statements)

References 41 publications

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“…25 Moreover, reduction of weaning-associated diarrhea, attenuation of intestinal inflammation, regulation of intestinal microbiota, and promotion of intestinal barrier function have been reported in weanling piglets following resveratrol administration. 26–28 However, it is still unclear how resveratrol protects pig intestine from disorders and maintains intestinal barrier function. In the present study, we hypothesized that resveratrol could protect intestinal epithelial cells from ETEC K88 infection.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells

Luo

Hai

et al. 2022

Food Funct.

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Section: Introductionmentioning

confidence: 99%

Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells

Luo

Hai

et al. 2022

Food Funct.

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“…Nrf2 and Keap1 form a conjugate, and then Nrf2 dissociates from the conjugate and stably translocates into the nucleus, driving expression of antioxidant enzymes genes [47,48]. In the oxidative stress model induced by diquat, Nrf2 signaling is essential for regulating the redox state of cells [49,50]. The results in this study showed that weaning caused the down-regulated expression of Nrf2 and NQO1 in the liver of piglets on the 3rd and 7th day after weaning, respectively.…”

Section: Discussionmentioning

confidence: 67%

Early Weaning Affects Liver Antioxidant Function in Piglets

Peng

et al. 2021

Animals

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This study examined the impact of early weaning on antioxidant function in piglets. A total of 40 Duroc × Landrace × Large White, 21-day-old piglets (half male and half female) were divided into suckling groups (SG) and weaning groups (WG). Piglets in WG were weaned at the 21st day, while the piglets in SG continued to get breastfed. Eight piglets from each group were randomly selected and slaughtered at 24th-day (SG3, WG3) and 28th-day old (SG7, WG7). The body weight, liver index, hepatocyte morphology, antioxidant enzymes activity, gene expression of antioxidant enzymes, and Nrf2 signaling in the liver of piglets were measured. The results showed that weaning caused decreased body weight (p < 0.01), lower liver weight (p < 0.01), and decreased the liver organ index (p < 0.05) of piglets. The area and size of hepatocytes in the WG group was smaller than that in the SG group (p < 0.05). We also observed that weaning reduced the activity of superoxide dismutase (SOD) and catalase (CAT) (p < 0.05) in the liver of piglets. Relative to the SG3 group, the gene expression of GSH-Px in liver of WG3 was significantly reduced (p < 0.05). The gene expression of Nrf2 in the SG3 group was higher than that in the WG3 group (p < 0.01). The gene expression of NQO1 in the SG7 group was higher than that in the WG7 group (p < 0.05). In conclusion, weaning resulted in lower weight, slowed liver development, and reduced antioxidant enzymes activity, thereby impairing liver antioxidant function and suppressing piglet growth.

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“…Consistent with these findings, the current study also showed that compared with saline control treatment, Ang II infusion markedly increases the expression levels of the proinflammatory cytokines IL-1β, TNF-α and IL-6 ( Figure 3B ), which supports the conclusion that Ang II stimulated macrophages to release these factors, which were responsible for the endothelial ROS response, in this experiment. Moreover, the administration of resveratrol effectively protects intestinal integrity; alleviates intestinal inflammation and oxidative stress by inhibiting the activity of SOD, GSH-Px, and CAT; and decreases MDA levels in the jejunal mucosa in diquat-challenged piglets ( Xun et al, 2021 ). Moreover, VCAM-1 can promote both intracellular and extracellular calcium flux and specifically activate the Rho family GTPase Rac1, which results in NOX activity and ROS production ( Cook-Mills et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Blocking VCAM-1 Prevents Angiotensin II-Induced Hypertension and Vascular Remodeling in Mice

Yin

Bai

et al. 2022

Front. Pharmacol.

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Adhesion of monocytes to the vascular endothelium frequently leads to an inflammatory response, which contributes to hypertension and vascular remodeling. Vascular cellular adhesion molecule-1 (VCAM-1) plays an important role in leukocyte adhesion and migration during inflammatory diseases. However, its role in angiotensin (Ang) II -induced hypertension and vascular dysfunction remains largely unknown. Wild-type (WT) mice were administered a VCAM-1 neutralizing antibody (0.1 or 0.2 mg/mouse/day) or IgG control and then infused with Ang II (490 ng kg−1 min−1) or saline continuously for 14 days. Systolic blood pressure (SBP) was measured with a tail-cuff system, pathological changes in the aorta were assessed by histological staining, and vascular relaxation was analyzed an aortic ring assay. Our results indicated that compared with saline infusion, Ang II infusion significantly upregulated VCAM-1 expression in the mouse aorta and serum. Moreover, Ang II infusion markedly increased arterial hypertension, wall thickness, fibrosis, infiltration of Mac-2+ macrophages, reactive oxygen species (ROS) production and vascular relaxation dysfunction. Conversely, blockade of VCAM-1 with a neutralizing antibody substantially alleviated these effects. In vitro experiments further confirmed that the VCAM-1 neutralizing antibody inhibited Ang II-induced macrophage adhesion and migration and DNA damage and oxidative stress in endothelial cells (ECs). In conclusion, these results indicate that blockade of VCAM-1 exerts a protective effect against Ang II-induced arterial hypertension and dysfunction by regulating monocytes adhesion and infiltration into the endothelium and represents a novel therapeutic approach for hypertension.

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Resveratrol protects intestinal integrity, alleviates intestinal inflammation and oxidative stress by modulating AhR/Nrf2 pathways in weaned piglets challenged with diquat

Cited by 37 publications

References 41 publications

Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells

Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells

Early Weaning Affects Liver Antioxidant Function in Piglets

Blocking VCAM-1 Prevents Angiotensin II-Induced Hypertension and Vascular Remodeling in Mice

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