Resveratrol Protects against 2-Bromopropane-Induced Apoptosis and Disruption of Embryonic Development in Blastocysts

Chan, Wen‐Hsiung

doi:10.3390/ijms12084991

Cited by 7 publications

(3 citation statements)

References 59 publications

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“…As oxidative stress plays critical roles in the apoptosis triggered by a number of chemical compounds [ 35 , 36 , 37 , 38 , 39 , 40 ], we investigated whether reactive oxygen species (ROS) could be involved in the rhein-induced apoptosis of blastocysts in vitro. Staining with dichlorodihydrofluorescein diacetate (DCFDA) fluorescence dye (which detects intracellular ROS levels) revealed that the rhein-treated group had significantly higher ROS levels compared to the untreated control group ( Figure 5 A,B).…”

Section: Resultsmentioning

confidence: 99%

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Huang

Chan

2017

IJMS

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Rhein, a glucoside chemical compound found in a traditional Chinese medicine derived from the roots of rhubarb, induces cell apoptosis and is considered to have high potential as an antitumor drug. Several previous studies showed that rhein can inhibit cell proliferation and trigger mitochondria-related or endoplasmic reticulum (ER) stress-dependent apoptotic processes. However, the side effects of rhein on pre- and post-implantation embryonic development remain unclear. Here, we show that rhein has cytotoxic effects on blastocyst-stage mouse embryos and induces oxidative stress and immunotoxicity in mouse fetuses. Blastocysts incubated with 5–20 μM rhein showed significant cell apoptosis, as well as decreases in their inner cell mass cell numbers and total cell numbers. An in vitro development assay showed that rhein affected the developmental potentials of both pre- and post-implantation embryos. Incubation of blastocysts with 5–20 μM rhein was associated with increased resorption of post-implantation embryos and decreased fetal weight in an embryo transfer assay. Importantly, in an in vivo model, intravenous injection of dams with rhein (1, 3, and 5 mg/kg body weight/day) for four days resulted in apoptosis of blastocyst-stage embryos, early embryonic developmental injury, and decreased fetal weight. Intravenous injection of dams with 5 mg/kg body weight/day rhein significantly increased the total reactive oxygen species (ROS) content of fetuses and the transcription levels of antioxidant proteins in fetal livers. Additional work showed that rhein induced apoptosis through ROS generation, and that prevention of apoptotic processes effectively rescued the rhein-induced injury effects on embryonic development. Finally, the transcription levels of the innate-immunity related genes, CXCL1, IL-1 β and IL-8, were down-regulated in the fetuses of dams that received intravenous injections of rhein. These results collectively show that rhein has the potential to induce embryonic cytotoxicity and induce oxidative stress and immunotoxicity during the development of mouse embryos.

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Section: Resultsmentioning

confidence: 99%

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Huang

Chan

2017

IJMS

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“…Resveratrol (0.5 μM) can also protect against oxidative damage in preimplantation embryos (Chan, 2011) and improve porcine and bovine preimplantation embryo development in vitro (Lee, et al, 2010, Salzano, et al, 2014, Wang, et al, 2014). A natural flavonoid, quercetin, can protect embryos from peroxide induced damage (Yu, et al, 2014).…”

Section: Interventions To Reduce Impact Of Er Stress Responsementioning

confidence: 99%

Stress signaling in mammalian oocytes and embryos: a basis for intervention and improvement of outcomes

Latham

2015

Cell Tissue Res

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Oocytes and early stage embryos are highly sensitive to variation in diverse exogenous factors such as temperature, osmolarity, oxygen, nutrient restriction, pH, shear stress, toxins, amino acid availability, and lipids. It is becoming increasingly apparent that many such factors negatively affect the endoplasmic reticulum, protein synthesis and protein processing, initiating ER stress and unfolded protein responses. As a result, ER stress signaling serves as a common mediator of cellular responses to diverse stressors. In oocytes and embryos, this leads to developmental arrest and epigenetic changes. Recent studies have revealed that preventing ER stress or inhibiting ER stress signaling can preserve or even enhance oocyte and embryo developmental potential. This chapter reviews ER stress signaling, how it arises, how it affects oocytes and embryos, and how its occurrence can be managed or prevented.

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“…In a study, blastocysts were exposed to 2‐bromopropane in which levels of implantation and ability to proceed to later stages of embryonic development were lowered. Moreover, 2‐bromopropane led to ROS production, loss of mitochondrial membrane potential, and activation of caspase 3, whereas RSV counteracted them (Chan, ).…”

Section: Chemical‐induced Toxicitymentioning

confidence: 99%

Protective effects of Vitis vinifera (grapes) and one of its biologically active constituents, resveratrol, against natural and chemical toxicities: A comprehensive review

Tabeshpour

Mehri

Behbahani

et al. 2018

Phytotherapy Research

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Vitis vinifera (grape) is one of the "most-produced fruit" in the world. Grape seeds are a valuable source of phenolic compounds including resveratrol (RSV). Grape and one of its biologically active constituents, RSV, exert their protective effects against different natural or chemical toxins which could alter physiological homeostasis through a variety of mechanisms. Some of these mechanisms of actions include increase in superoxide dismutase, hemeoxygenase-1, and glutathione peroxidase activities and reduced glutathione content and decrease in malondialdehyde (MDA) levels and activation of the nuclear erythroid2-related factor2/ARE pathway. There are also various reports of the potential use of such compounds in preventing different ailments including cardiovascular diseases, cancer, degenerative diseases, and inflammatory disorders. Therefore, in this review, we have investigated the possible protective effects of grape and one of its biologically active constituents, RSV, on different organs' toxicity induced by natural toxins (such as mycotoxins, lipopolysaccharide, and triptolide) and chemical toxins (such as antitumors, metals, and carbon tetrachloride). There are insufficient clinical trials on this subject, so our review only includes in vivo and in vitro studies. To establish the grape beneficial effects in human intoxication, more clinical trials need to be accomplished.

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Resveratrol Protects against 2-Bromopropane-Induced Apoptosis and Disruption of Embryonic Development in Blastocysts

Cited by 7 publications

References 59 publications

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Rhein Induces Oxidative Stress and Apoptosis in Mouse Blastocysts and Has Immunotoxic Effects during Embryonic Development

Stress signaling in mammalian oocytes and embryos: a basis for intervention and improvement of outcomes

Protective effects of Vitis vinifera (grapes) and one of its biologically active constituents, resveratrol, against natural and chemical toxicities: A comprehensive review

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