Resveratrol promotes mammary cell proliferation and antioxidation capacity during pregnancy and lactation in mice

Abstract

Aims Reproduction can induce maternal oxidative stress, and resveratrol is able to alleviate oxidative stress. This study aimed to investigate the protective effects of resveratrol supplementation in mice during pregnancy and lactation. Methods and Results Pregnant mice were randomly divided into three groups: (i) Pure water (Con); (ii) Cellulose sodium dissolves with 20 mg kg−1 resveratrol (R1) and (iii) Cellulose sodium dissolves with 40 mg kg−1 resveratrol (R2). The experiment starts at pregnancy and ends at weaning. The results showed that resveratrol increased mammary cell proliferation and the mRNA expressions of UDP glucuronosyltransferase family 1 member A1 (UGT1A1) and cytochrome P450 proteins (CYP1A1), while decreased superoxide dismutase, extracellular (SOD3) in the mammary gland. Further study showed that resveratrol promoted the mRNA expressions of genes involved in mitophagy, such as transcription factor Eβ (Tfeβ), Bcl1lc3β, homosapiens microtubule‐associated protein 1 light‐chain 3 beta (Map1lc3β), Parkin, sequestosome1 (p62), autophagy‐related protein (Atg5) and Beclin‐1 (Becn1) in the mammary gland. Moreover, resveratrol increased the abundances of some intestinal microbial species. 40 mg kg−1 resveratrol significantly increased the contents of Acidobacteri, unidentified acidbacteria, Bacilales, Staphylococcaceae and Staphylococcus at phylum, class, order, family and genus level respectively. Conclusion Our results indicate that resveratrol supplementation may promote mammary cell proliferation and antioxidant ability through mitophagy and regulating gut microbiota in pregnant mice. Significance and Impact of the Study Current study proved that resveratrol could affect mammary cell proliferation and antioxidation capacity during pregnancy and lactation in mice for the first time. The underlying mechanisms may be related to mitophagy and gut microbiota.

Resveratrol Butyrate Esters Inhibit Obesity Caused by Perinatal Exposure to Bisphenol A in Female Offspring Rats

“…Huang et al [ 75 ] showed that RSV supplementation could not alleviate the reduction in the abundance of Lactobacillus in offspring caused by prenatal and postnatal HFDs. Zha et al (2020) revealed that the offspring of normal mice treated with 40 mg/kg/day of RSV during pregnancy displayed a decreasing trend in the abundance of Lactobacillus in their intestinal tracts [ 76 ]. The results of these previous studies were consistent with those of this study ( Figure 3 B,C), i.e., the results of the operational taxonomic units (OTUs) showed that RBE intake can increase the abundance of S24-7 and decrease the abundance of Lactobacillus.…”

Polyphenols and Lactation: Molecular Evidence to Support the Use of Botanical Galactagogues

“…[ 124 ] Zha and colleagues fed mice resveratrol through pregnancy and lactation and observed increased luminal cell density and increased expression of detoxifying enzymes (UDP glucuronosyltransferase 1, UGT1A1 ; cytochrome P450, CYP1A1 ). Additionally expression of mitophagy genes, such as transcription factor Eβ (Tfeβ), Bcl1lc3β, (Map1lc3β), Parkin, sequestosome1 (p62), autophagy‐related protein ( ATG5 ), and Beclin‐1 ( BECN1 ) are increased in the mammary gland in response to resveratrol, [ 125 ] all of which are important for MEC differentiation into lactocytes. [ 126 ] Resveratrol also prevents H 2 O 2 ‐induced endoplasmic reticulum stress and mitochondria‐related cell apoptosis in MAC‐T cells through decreased expression of GRP8, CHOP, BAX , and BCL2 in a nuclear factor erythroid 2‐related factor 2 (Nrf2)‐dependent manner, through the prolonged activation of insulin signaling pathways such as PI3K/Akt and ERK/MAPK.…”

Effect of Resveratrol on Pregnancy, Prenatal Complications and Pregnancy-Associated Structure Alterations

“…Mitophagy is defined as the selective removal of dysfunctional mitochondria by autophagy, a process aberrantly affected by ROS increase and physiologically restored by oxidative stress balance [69]. Consonantly, a study by Zha et al [70] demonstrated that resveratrol supplementation in pregnant mice might promote mammary gland proliferation and antioxidant activity through mitophagy activation [70]. Pregnancy-related metabolic disorders, such as GDM, occur when ROS prevents insulin from facilitating cellular glucose uptake, subsequently leading to insulin resistance [71].…”