Resveratrol lacks antifungal activity against Candida albicans

Collado-González, Mar; Guirao-Abad, José P.; Sánchez-Fresneda, Ruth; Belchí-Navarro, Sarai; Argüelles, Juan-Carlos

doi:10.1007/s11274-012-1042-1

Cited by 21 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, a study indicates that resveratrol is not effective against both C. albicans and non- C. albicans species [ 101 ]. In another study, resveratrol antifungal activity against C. albicans could be reached at 400 μg/mL, thereby minimizing the antifungal role of resveratrol against C. albicans -caused infections [ 103 ].…”

Section: Biological Activities Of Resveratrolmentioning

confidence: 99%

Resveratrol: A Double-Edged Sword in Health Benefits

et al. 2018

View full text Add to dashboard Cite

Resveratrol (3,5,4′-trihydroxy-trans-stilbene) belongs to polyphenols’ stilbenoids group, possessing two phenol rings linked to each other by an ethylene bridge. This natural polyphenol has been detected in more than 70 plant species, especially in grapes’ skin and seeds, and was found in discrete amounts in red wines and various human foods. It is a phytoalexin that acts against pathogens, including bacteria and fungi. As a natural food ingredient, numerous studies have demonstrated that resveratrol possesses a very high antioxidant potential. Resveratrol also exhibit antitumor activity, and is considered a potential candidate for prevention and treatment of several types of cancer. Indeed, resveratrol anticancer properties have been confirmed by many in vitro and in vivo studies, which shows that resveratrol is able to inhibit all carcinogenesis stages (e.g., initiation, promotion and progression). Even more, other bioactive effects, namely as anti-inflammatory, anticarcinogenic, cardioprotective, vasorelaxant, phytoestrogenic and neuroprotective have also been reported. Nonetheless, resveratrol application is still being a major challenge for pharmaceutical industry, due to its poor solubility and bioavailability, as well as adverse effects. In this sense, this review summarized current data on resveratrol pharmacological effects.

show abstract

Section: Biological Activities Of Resveratrolmentioning

confidence: 99%

Resveratrol: A Double-Edged Sword in Health Benefits

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The effect of resveratrol (3,5,4-trihydroxystilbene), a close analogue of PTE, on C. albicans has been investigated previously (57)(58)(59). However, its antifungal effect was much weaker than PTE.…”

Section: Met14mentioning

confidence: 99%

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Zhao

Mylonakis

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

d Pterostilbene (PTE) is a stilbene-derived phytoalexin that originates from several natural plant sources. In this study, we evaluated the activity of PTE against Candida albicans biofilms and explored the underlying mechanisms. In 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assays, biofilm biomass measurement, confocal laser scanning microscopy, and scanning electron microscopy, we found that <16 g/ml PTE had a significant effect against C. albicans biofilms in vitro, while it had no fungicidal effect on planktonic C. albicans cells, which suggested a unique antibiofilm effect of PTE. Then we found that PTE could inhibit biofilm formation and destroy the maintenance of mature biofilms. At 4 g/ ml, PTE decreased cellular surface hydrophobicity (CSH) and suppressed hyphal formation. Gene expression microarrays and real-time reverse transcription-PCR showed that exposure of C. albicans to 16 g/ml PTE altered the expression of genes that function in morphological transition, ergosterol biosynthesis, oxidoreductase activity, and cell surface and protein unfolding processes (heat shock proteins). Filamentation-related genes, especially those regulated by the Ras/cyclic AMP (cAMP) pathway, including ECE1, ALS3, HWP1, HGC1, and RAS1 itself, were downregulated upon PTE treatment, indicating that the antibiofilm effect of PTE was related to the Ras/cAMP pathway. Then, we found that the addition of exogenous cAMP reverted the PTE-induced filamentous growth defect. Finally, with a rat central venous catheter infection model, we confirmed the in vivo activity of PTE against C. albicans biofilms. Collectively, PTE had strong activities against C. albicans biofilms both in vitro and in vivo, and these activities were associated with the Ras/cAMP pathway.

show abstract

“…Previously, Collado-González et al suggested that resveratrol did not possess critical antifungal and killing effects against C. albicans even in high concentration [8]. However, in this study, we focused on the intracellular mechanism of resveratrol in C. albicans.…”

Section: Resveratrol Induces Apoptosis Through the Caspasedependent Pmentioning

confidence: 81%

Novel Antifungal Mechanism of Resveratrol: Apoptosis Inducer in Candida albicans

Lee

2014

Curr Microbiol

View full text Add to dashboard Cite

Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a well-known natural polyphenolic compound that has garnered considerable interest because of its bioavailability and pharmacokinetics in humans. Although the antimicrobial activity of resveratrol has recently been focused, however, the antifungal activity and its mechanism are still largely unknown. Here, we report for the first time the potential of resveratrol as an apoptosis inducer in the human pathogenic fungus Candida albicans. The results showed that resveratrol exerted its effects from the early to the late stages of apoptosis and involved the activity of reactive oxygen species, particularly hydroxyl radicals ((∙)OH). DiOC6(3) and JC-1 staining indicated that loss of mitochondrial membrane potential (ΔΨ m) is a key event in resveratrol-induced apoptosis. Finally, we investigated metacaspase activation resulting from mitochondrial dysfunction. The result showed that resveratrol strongly activated metacaspase and promoted cytochrome c release. In summary, resveratrol induces fungal apoptosis through a caspase-dependent mitochondrial pathway and is a potential agent for treating human fungal diseases.

show abstract

Resveratrol lacks antifungal activity against Candida albicans

Cited by 21 publications

References 25 publications

Resveratrol: A Double-Edged Sword in Health Benefits

Resveratrol: A Double-Edged Sword in Health Benefits

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Novel Antifungal Mechanism of Resveratrol: Apoptosis Inducer in Candida albicans

Contact Info

Product

Resources

About