Resveratrol inhibits the proliferation of human melanoma cells by inducing G1/S cell cycle arrest and apoptosis

Abstract

Resveratrol (Res), a natural plant extract, is an effective inducer of cell apoptosis and cell cycle arrest in multiple carcinoma cell types, which has been demonstrated by its ability to inhibit the proliferation of multiple human tumor cells in vitro. Although Res possesses chemopreventive properties against several types of cancer, the molecular mechanism underlying its anticancer activity remains to be fully elucidated. The present study demonstrated that Res induced cell cycle arrest and inhibited the proliferation of human melanoma A375 (IC50=23 µM after 48 h; P<0.05) and SK-MEL-31 (IC50=15 µM after 48 h; P<0.05) cells. Western blot analysis demonstrated that Res induced the apoptosis of human melanoma A375 and SK-MEL-31 cells by upregulating the expression of Bcl-2-associated X protein and B-cell lymphoma 2, possibly via the p53 pathway and activation of caspase-9 and caspase-3.

Inhibitory Effects of Oligostilbenoids from the Bark of <i>Shorea roxburghii</i> on Malignant Melanoma Cell Growth: Implications for Novel Topical Anticancer Candidates

“…A recent study has demonstrated that treatment of human melanoma cells with resveratrol resulted in inhibition of cell proliferation by inducing G1/S cell cycle arrest and apoptosis, possibly via the p53 pathway. 19) Our present data indicated that resveratrol treatment triggered cell cycle arrest at the G1 phase possibly via the upregulation of p21 and the induction of apoptosis (Fig. 5).…”

Pro-apoptotic and anti-proliferative effects of corn silk extract on human colon cancer cell lines

“…It was demonstrated that the change in mitochondrial outer membrane permeability could lead to apoptosis, and this change could be directly mediated by the Bcl-2 family proteins. In addition, the main activity of the Bcl-2 family proteins takes place on the mitochondrial membrane (27). In the present study, the decrease in the Bcl-2/Bax ratio might have led to a decrease in ΔΨm and subsequent activation of caspase-3 via the cytochrome c and caspase-9 pathway.…”

Folic acid-functionalized drug delivery platform of resveratrol based on Pluronic 127/D-&alpha;-tocopheryl polyethylene glycol 1000 succinate mixed micelles

“…1 Some literature have illustrated that resveratrol can both inhibit tumor cell growth and promote cell apoptosis exerting its antitumor effect by means of some related cell signaling pathways. 2,3 However, poor bioavailability of resveratrol owing to factors such as low aqueous solubility, easy photodegradation and extensive first-pass metabolism hinders its further clinical application. 4 Therefore, the primary problem is how to design effective and suitable drug delivery systems (DDSs) for resveratrol to address the aforementioned shortcomings.…”