Resveratrol inhibits TGF-β1-induced epithelial-to-mesenchymal transition and suppresses lung cancer invasion and metastasis

Wang, Heyong; Zhang, Huijun; Tang, Liang; Chen, Hạixia; Wu, Chen; Zhao, Ming; Yang, Yaoqin; Chen, Xiaofeng; Liu, Gentao

doi:10.1016/j.tox.2012.09.017

Cited by 146 publications

(99 citation statements)

References 50 publications

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“…These provided a novel perspective on the role of resveratrol in preventing cancer progression as an EMT inhibitor. Experiments on the lung cancer cell line A549 also supported this conclusion (79).…”

Section: Resveratrol Affects Emtmentioning

confidence: 72%

Anti-tumor effects and cellular mechanisms of resveratrol

Han

Xia

Gao

et al. 2015

DD&T

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Section: Resveratrol Affects Emtmentioning

confidence: 72%

Anti-tumor effects and cellular mechanisms of resveratrol

Han

Xia

Gao

et al. 2015

DD&T

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“…In particular, study findings have indicated that resveratrol inhibits EMT in lung, pancreatic and breast cancer (29)(30)(31), and that it is also able to suppress the Hh signaling pathway in prostate carcinoma (32). In addition, resveratrol has been identified as being able to prevent, reverse or delay carcinogenesis.…”

Section: Resveratrol Inhibits the Hedgehogmentioning

confidence: 99%

Resveratrol inhibits the hedgehog signaling pathway and epithelial-mesenchymal transition and suppresses gastric cancer invasion and metastasis

et al. 2015

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Abstract. The hedgehog (Hh) signaling pathway is vital to vertebrate development, the homeostatic process and tumorigenesis. Epithelial-mesenchymal transition (EMT) is a cellular process during which epithelial cells become mesenchymal-appearing cells, which in turn promotes cancer metastasis and invasion. Resveratrol is a natural polyphenolic compound found in grapes, a variety of berries, peanuts and other plants. Numerous studies have demonstrated that the Hh signaling pathway is able to regulate the EMT, and that resveratrol can suppress carcinoma invasion and metastasis. In addition, certain studies have indicated that resveratrol can inhibit the Hh signaling pathway and EMT in cancers other than gastric cancer. The purpose of the present study was to investigate the inhibitory effect of resveratrol on the Hh signaling pathway and EMT in gastric cancer in vitro. Gastric cancer SGC-7901 cells were treated with resveratrol or cyclopamine at different concentrations. The viability of the cells was assessed using an MTT assay. The expression of Gli-1, a key component of the Hh signaling pathway, and Snail, E-cadherin and N-cadherin, key components of EMT, was detected by reverse transcription polymerase chain reaction (RT-PCR) and western blotting. The invasion and metastasis of the cells were observed by performing a cell scratch test. The RT-PCR and western blotting showed a decrease in Gli-1, Snail and N-cadherin expression, and an increase in E-cadherin expression in the resveratrol and cyclopamine group compared with the control group, suggesting that resveratrol inhibited the Hh pathway and EMT, as did cyclopamine. The MTT assay indicated that the viability of the SGC-7901 cells was significantly decreased in a concentration-dependent manner following resveratrol and cyclopamine treatment. The cell scratch test showed slower cell invasion and metastasis in the resveratrol and cyclopamine groups. These findings indicated that resveratrol was able to inhibit the Hh signaling pathway and EMT, and suppress invasion and metastasis in gastric cancer in vitro.

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“…In agreement with previous studies [62,74], we successfully used 5 ng/mL TGFβ1 in serum-free media to induce EMT in H1299 and A549 NSCLC cells. Our findings indicate the TGFβ1 decreased the expression of E-cadherin in both A549…”

Section: Discussionsupporting

confidence: 88%

Withaferin a synergistically enhances the effects of paclitaxel against lung cancer.

Kyakulaga¹

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Resveratrol inhibits TGF-β1-induced epithelial-to-mesenchymal transition and suppresses lung cancer invasion and metastasis

Cited by 146 publications

References 50 publications

Anti-tumor effects and cellular mechanisms of resveratrol

Anti-tumor effects and cellular mechanisms of resveratrol

Resveratrol inhibits the hedgehog signaling pathway and epithelial-mesenchymal transition and suppresses gastric cancer invasion and metastasis

Withaferin a synergistically enhances the effects of paclitaxel against lung cancer.

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