Resveratrol inhibits proliferation, migration and invasion of multiple myeloma cells via NEAT1-mediated Wnt/β-catenin signaling pathway

Geng, Wenhua; Guo, Xin; Zhang, Lirong; Ma, Yiming; Wang, Lei; Liu, Zhen; Ji, Hong; Xiong, Ying

doi:10.1016/j.biopha.2018.08.003

Cited by 82 publications

(51 citation statements)

References 28 publications

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“…It should be pointed out that antimigratory actions of RESV were less pronounced in our cell-line assays than in the tissue culture capsular bag model. Previous work also has indicated that RESV can affect migration of various cancer cells, 24,25 and it has been suggested that RESV may inhibit migration of ARPE-19 retinal epithelial cells. 26 RESV treatment resulted in a significant reduction in transdifferentiation from lens epithelial cells to myofibroblasts.…”

Section: Discussionmentioning

confidence: 95%

Resveratrol Inhibits Wound Healing and Lens Fibrosis: A Putative Candidate for Posterior Capsule Opacification Prevention

Smith

Eldred

Wormstone

2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Posterior capsule opacification (PCO) is a common complication of cataract surgery. In addition to improved surgical methods and IOL designs, it is likely additional agents will be needed to improve patient outcomes. Presently no pharmacological agent is in clinical use to prevent PCO. Here we investigate the putative ability of resveratrol (RESV), a naturally occurring polyphenol, as a therapeutic agent. METHODS. The human lens epithelial cell line FHL124, a human lens capsular bag model, and central anterior epithelium were used as experimental systems. Standard culture was in 5% fetal calf serum Eagle's minimum essential medium; 10 ng/mL transforming growth factor-b2 (TGFb2) was used to induce fibrotic changes. A scratch wound assay was used to measure cell migration and the patch assay was used to assess matrix contraction by FHL124 cells. Protein expression was assessed by immunocytochemistry and Western blot and gene expression by quantitative RT-PCR. In capsular bags, cell growth across the posterior lens capsule, capsular wrinkling, and epithelial-to-mesenchymal transition were determined by image analysis. RESULTS. In FHL124 cells, addition of 30 lM RESV significantly impeded cell migration in a wound-healing assay. RESV significantly inhibited TGFb2-induced expression of the myofibroblast marker alpha-smooth muscle actin (a-SMA) at both the message and protein levels, as well as significantly inhibiting matrix contraction induced by TGFb2. In human capsular bags, 30 lM RESV significantly inhibited cell growth. TGFb2-induced a-SMA expression and capsular wrinkling were also significantly inhibited by RESV treatment. RESV significantly suppressed expression of TGFb2-induced genes associated with fibrotic disease, including matrix metalloproteinase-2 in FHL124 cells, capsular bags, and central anterior epithelium. CONCLUSIONS. RESV can counter PCO-related physiological events in two human lens model systems. RESV therefore has the potential to be used as a candidate agent for the prevention of PCO, which in turn could benefit millions of cataract patients.

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Section: Discussionmentioning

confidence: 95%

Resveratrol Inhibits Wound Healing and Lens Fibrosis: A Putative Candidate for Posterior Capsule Opacification Prevention

Smith

Eldred

Wormstone

2019

Invest. Ophthalmol. Vis. Sci.

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“…In contrast, knockdown studies indicated that loss of Neat1 suppressed neuronal differentiation and migration, but promoted apoptosis. Neat1 is a crucial regulator of Wnt/β-catenin [31]. We therefore hypothesized that Neat1 might boost neuronal differentiation and migration and inhibit apoptosis in a Wnt/β-catenin signaling-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

LncRNA Neat1 mediates miR-124-induced activation of Wnt/β-catenin signaling in spinal cord neural progenitor cells

et al. 2019

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Background: Emerging evidence suggests that miR-124 performs important biological functions in neural stem cells (NSCs); it regulates NSC behavior and promotes the differentiation of NSCs into neurons, but the exact molecular mechanism remains unknown. And also, the role of miR-124 during spinal cord injury regeneration is unclear. Materials and methods: In order to explore the function of miR-124 in neural differentiation, the molecular markers (Tuj1, Map2, and GFAP) correlated with the differentiation of NSCs were detected by immunofluorescence staining both in cultured mouse spinal cord progenitor cells (SC-NPCs) and in spinal cord injury (SCI) animal models. The migration ability and apoptosis of cultured SC-NPCs were also evaluated by Transwell migration assay and TUNEL assay. In addition, the relative expression of lnRNA Neat1-and Wnt/β-catenin signaling-related genes were detected by quantitative real-time PCR. Results: In this study, we revealed that lncRNA Neat1 is involved in regulating Wnt/β-catenin signaling that is activated by miR-124 in SC-NPCs. LncRNA Neat1 was also found to play an important role in regulating neuronal differentiation, apoptosis, and migration of SC-NPCs. Furthermore, we demonstrated that overexpression of miR-124 resulted in elevated Neat1 expression, accompanied with the functional recovery of locomotion in a mouse model of spinal cord injury. Conclusions: Our results confirm the therapeutic effectiveness of miR-124 on the functional recovery of injured spinal cord, supporting the rationale and feasibility of miR-124 for spinal cord injury treatment in future clinical therapy. Furthermore, we concluded that the miR-124-Neat1-Wnt/β-catenin signaling axis is involved in regulating the cell function of SC-NPCs, and this may offer novel therapeutic avenues for future treatment of SCI.

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“…For example, metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is overexpressed in MM tissues and various MM cell lines; upregulation of MALAT1 is significantly associated with poor prognosis, including overall survival (oS) and progression-free survival (PFS) (31)(32)(33). nuclear paraspeckle assembly transcript 1 (NEAT1) has also been reported to serve a pivotal role in promoting MM, and its elevated expression is closely associated with poor prognosis (34,35). The upregulation of urothelial cancer associated 1 (UCA1) (36), protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P) (37), H19 (38), colon cancer associated transcript 1 (CCAT1) (39) and colorectal neoplasia differentially expressed (CRNDE) (40) are closely associated with poor prognosis in MM; these genes may be used as future indicators in the clinical prognosis of patients with MM.…”

Section: Introductionmentioning

confidence: 99%

Construction of a prognosis‑associated long noncoding RNA‑mRNA network for multiple myeloma based on microarray and bioinformatics analysis

Zhu

Wang

Ye³

et al. 2020

Mol Med Report

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at present, t he associat ion bet we en prognosis-associated long noncoding rnas (lncrnas) and mrnas is yet to be reported in multiple myeloma (MM). The aim of the present study was to construct prognostic models with lncrnas and mrnas, and to map the interactions between these lncrnas and mrnas in MM. lncrna and mrna data from 559 patients with MM were acquired from the Genome expression omnibus (dataset GSe24080), and their prognostic values were calculated using the survival package in r. Multivariate Cox analysis was used on the top 20 most significant prognosis-associated mrnas and lncrnas to develop prognostic signatures. The performances of these prognostic signatures were tested using the survivalroc package in r, which allows for time-dependent receiver operator characteristic (roc) curve estimation. Weighted correlation network analysis (WGcna) was conducted to investigate the associations between lncrnas and mrnas, and a lncrna-mrna network was constructed using cytoscape software. univariate cox regression analysis identified 39 lncRNAs and 1,445 mRNAs that were significantly associated with event-free survival of MM patients. The top 20 most significant survival-associated lncrnas and mrnas were selected as candidates for analyzing independent MM prognostic factors. Both signatures could be used to separate patients into two groups with distinct outcomes. The areas under the ROC curves were 0.739 for the lncRNA signature and 0.732 for the mRNA signature. In the lncRNA-mRNA network, a total of 143 mRNAs were positively or negatively associated with 23 prognosis-associated lncRNAs. NCRNA00201, LOC115110 and RP5-968J1.1 were the most dominant drivers. The present study constructed a model that predicted prognosis in MM and formed a network with the corresponding prognosis-associated mrnas, providing a novel perspective for the clinical diagnosis and treatment of MM, and suggesting novel directions for interpreting the mechanisms underlying the development of MM.

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Resveratrol inhibits proliferation, migration and invasion of multiple myeloma cells via NEAT1-mediated Wnt/β-catenin signaling pathway

Cited by 82 publications

References 28 publications

Resveratrol Inhibits Wound Healing and Lens Fibrosis: A Putative Candidate for Posterior Capsule Opacification Prevention

Resveratrol Inhibits Wound Healing and Lens Fibrosis: A Putative Candidate for Posterior Capsule Opacification Prevention

LncRNA Neat1 mediates miR-124-induced activation of Wnt/β-catenin signaling in spinal cord neural progenitor cells

Construction of a prognosis‑associated long noncoding RNA‑mRNA network for multiple myeloma based on microarray and bioinformatics analysis

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