Resveratrol Inhibits Nicotinic Stimulation-Evoked Catecholamine Release from the Adrenal Medulla

Abstract

Resveratrol has been known to possess various potent cardiovascular effects in animal, but there is little information on its functional effect on the secretion of catecholamines (CA) from the perfused model of the adrenal medulla. Therefore, the aim of the present study was to determine the effect of resveratrol on the CA secretion from the isolated perfused model of the normotensive rat adrenal gland, and to elucidate its mechanism of action. Resveratrol (

Blockade by Nanomolar Resveratrol of Quantal Catecholamine Release in Chromaffin Cells

“…In their study on perfused rat adrenals, Woo et al (2008) also found that high resveratrol concentrations (10 -100 M) block bulk catecholamine release measured in 4-min periods. On the basis of experiments performed with BayK8644, these authors also suggest that resveratrol could be blocking the L-subtype of voltage-dependent calcium channels to inhibit secretion.…”

“…In the first study, Shinohara et al (2007) analyzed the release of catecholamine in primary cultures of bovine adrenal chromaffin cells elicited by acetylcholine (ACh), high potassium concentrations (K ϩ ), and veratridine; at micromolar concentrations, resveratrol blocked the responses to the three secretagogues. In a second study, Woo et al (2008) explored the effects of resveratrol on catecholamine release from perfused rat adrenal glands stimulated with ACh, dimethylphenylpiperazinium, K ϩ , the muscarinic receptor agonist 4- [[[(3-chlorophenyl)amino]carbonyl] oxy]-N,N,N-trimethyl-2-butyn-1-aminium chloride (MCN-A-343), veratridine, cyclopiazonic acid, and 1,4-dihydro-2, 6-dimethyl-5-nitro-4-[2-(trifluoromethyl) phenyl]-3-pyridinecarboxylic acid, methyl ester (BayK8644), an activator of chromaffin cell L-type calcium channels (García et al, 1984). The authors found that very high resveratrol concentrations (10 -100 M) reduced approximately 30 to 50% of the secretory responses elicited by 2-to 4-min perfusion of distinct secretagogues.…”

“…The authors found that very high resveratrol concentrations (10 -100 M) reduced approximately 30 to 50% of the secretory responses elicited by 2-to 4-min perfusion of distinct secretagogues. In most studies trying to decipher the mechanisms involved in the putative cardioprotective effects of resveratrol, including those of Shinohara et al (2007) and Woo et al (2008) on adrenal catecholamine release, high concentrations (up to 100 M) of the polyphenol were used. Considering that only trace amounts of resveratrol have been found in plasma after its oral administration (Walle et al, 2004), that orally administered resveratrol to humans is quickly metabolized into sulfate and glucuronic acid resveratrol-derivatives (Walle et al, 2004); and that after long-term consumption of moderate amounts of red wine, blood levels of resveratrol range from 100 to 1000 nM (Bertelli, 2006), it follows that in vitro biological effects of micromolar resveratrol cannot explain the putative cardioprotective effects of moderate consumption of red wine.…”

Sex/Gender- and Age-Related Differences in β-Adrenergic Receptor Signaling in Cardiovascular Diseases

“…Among the PhEs tested, resveratrol is a well-documented cardioprotective molecule impacting catecholamine synthesis and β-ARs. For instance, Woo et al reported that resveratrol, perfused into an adrenal vein of normotensive rats, inhibited the acetylcholine-induced secretion of catecholamines [ 161 ]. Similar data were obtained in vitro in primary bovine adrenal medullary cells by Shinohara et al [ 162 ] and Fernández-Morales and colleagues [ 163 ].…”