Chronic resveratrol consumption prevents hypertension development altering electrophysiological currents and Ca2+ signaling in chromaffin cells from SHR rats

Bomfim, Guilherme Henrique Souza; Musial, Diego Castro; Méndez-López, Iago; Jurkiewicz, Aron; Jurkiewicz, Neide Hyppolito; Padín, Juan Fernando; Garcı́a, Antonio G.

doi:10.1016/j.cellsig.2020.109811

Cited by 11 publications

(6 citation statements)

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Section: Methodsmentioning

confidence: 99%

“…All data, mathematical analyses, and graphs were analyzed and/or generated using the GraphPad Prism software version 9.4.1 (Inc., California, USA), as we previously described. 38 , 52 , 57 Basal c Ca 2+ levels were calculated averaging the values from 0 s to 60 s of each independent experiments. The Ca 2+ clearance through PMCAs was analyzed measuring the Ca 2+ efflux (clearance) rate parameter calculated in each individual trace and fitted by the nonlinear regression curve.…”

Section: Methodsmentioning

confidence: 99%

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PMCA Ca ²⁺ clearance in dental enamel cells depends on the magnitude of cytosolic Ca ²⁺

2022

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Enamel formation (amelogenesis) is a two‐step process whereby crystals partially grow during the secretory stage followed by a significant growth expansion during the maturation stage concurrent with an increase in vectorial Ca2+ transport. This requires tight regulation of cytosolic Ca2+ (cCa2+) concentration in the enamel forming ameloblasts by controlling Ca2+ influx (entry) and Ca2+ extrusion (clearance). Gene and protein expression studies suggest that the plasma membrane Ca2+‐ATPases (PMCA1‐4) are likely involved in cCa2+ extrusion in ameloblasts, yet no functional analysis of these pumps has been reported nor whether their activity changes across amelogenesis. PMCAs have high Ca2+ affinity and low Ca2+ clearance which may be a limiting factor in their contribution to enamel formation as maturation stage ameloblasts handle high Ca2+ loads. We analyzed PMCA function in rat secretory and maturation ameloblasts by blocking or potentiating these pumps. Low/moderate elevations in cCa2+ measured using the Ca2+ probe Fura‐2‐AM show that secretory ameloblasts clear Ca2+ faster than maturation stage cells through PMCAs. This process was completely inhibited by an external alkaline (pH 9.0) solution or was significantly delayed by the PMCA blockers vanadate and caloxin 1b1. Eliciting higher cCa2+ transients via the activation of the ORAI1 Ca2+ channel showed that the PMCAs of maturation ameloblasts were more efficient. Inhibiting PMCAs decreased the rate of Ca2+ influx via ORAI1 but potentiation with forskolin had no effect. Our findings suggest that PMCAs are functional Ca2+ pumps during amelogenesis regulating cCa2+ upon low and/or moderate Ca2+ stimulus in secretory stage, thus participating in amelogenesis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

PMCA Ca ²⁺ clearance in dental enamel cells depends on the magnitude of cytosolic Ca ²⁺

2022

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“…Reduced systolic and diastolic blood pressure, without altering heart rate, is also reported in deoxycorticosterone acetate (DOCA)-salt hypertensive mice upon resveratrol administration and is attributed to AMPK activation [189]. On the other hand, in spontaneously hypertensive rats long-term treatment with resveratrol induces reduction of systolic blood pressure through altering electrophysiological currents and Ca2+ signaling in sympathoadrenal chromaffin cells [190]. Resveratrol-induced reduction of systolic blood pressure by 5 mmHg was reported in clinical study involving obese humans [191].…”

Section: 44mentioning

confidence: 97%

Role of Resveratrol in Prevention and Control of Cardiovascular Disorders and Cardiovascular Complications Related to Covid-19 Disease: Mode of Action and Approaches Explored to Increase Its Bioavailability

Gligorijević¹,

Stanić-Vučinić²,

Radomirović³

et al. 2021

Preprint

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Resveratrol is a phytoalexin produced by many plants as a defense mechanism against stress-inducing conditions. The richest dietary sources of resveratrol are berries and grapes, their juices and wines. Good bioavailability of resveratrol is not reflected in its high biological activity in vivo because of resveratrol isomerization and its poor solubility in aqueous solutions. Proteins, cyclodextrins and nanomaterials have been explored as innovative delivery vehicles for resveratrol to overcome this limitation. Numerous in vitro and in vivo studies demonstrated beneficial effects of resveratrol in cardiovascular diseases (CVD). Main beneficial effects of resveratrol intake are cardioprotective, anti-hypertensive, vasodilatory, anti-diabetic, and improvement of lipid status. As resveratrol can alleviate the numerous factors associated with CVD, it has potential as a functional supplement to reduce COVID-19 illness severity in patients displaying poor prognosis due to cardio-vascular complications. Resveratrol was shown to mitigate the major pathways involved in the pathogenesis of SARS-CoV-2 including regulation of the renin-angiotensin system and expression of angiotensin-converting enzyme 2, stimulation of immune system and downregulation of pro-inflammatory cytokines release. Therefore, several studies already have anticipated potential implementation of resveratrol in COVID-19 treatment. Regular intake of resveratrol rich diet, or resveratrol-based complementary medicaments, may contribute to a healthier cardio-vascular system, prevention and control of CVD, including COVID-19 disease related complications of CVD.

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“…The study exhibited an increase in outward voltage-dependent potassium currents (I K ), a reduction in inward voltage-dependent sodium (I Na ), calcium (I Ca ), and nicotinic (I Ach ) currents, and an attenuation of cytosolic Ca 2+ concentration overload in chromaffin cells from SHR rats. Based on these findings, the modulation of the sympathoadrenal axis functionality may be a new target that could account for resveratrol's antihypertensive effect [93].…”

Section: Resveratrolmentioning

confidence: 99%

Biological Potential of Polyphenols in the Context of Metabolic Syndrome: An Analysis of Studies on Animal Models

Niewiadomska

Gajek-Marecka

Gajek

et al. 2022

Biology

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Metabolic syndrome (MetS) is a disease that has a complex etiology. It is defined as the co-occurrence of several pathophysiological disorders, including obesity, hyperglycemia, hypertension, and dyslipidemia. MetS is currently a severe problem in the public health care system. As its prevalence increases every year, it is now considered a global problem among adults and young populations. The treatment of choice comprises lifestyle changes based mainly on diet and physical activity. Therefore, researchers have been attempting to discover new substances that could help reduce or even reverse the symptoms when added to food. These attempts have resulted in numerous studies. Many of them have investigated the bioactive potential of polyphenols as a “possible remedy”, stemming from their antioxidative and anti-inflammatory effects and properties normalizing carbohydrate and lipid metabolism. Polyphenols may be supportive in preventing or delaying the onset of MetS or its complications. Additionally, the consumption of food rich in polyphenols should be considered as a supplement for antidiabetic drugs. To ensure the relevance of the studies on polyphenols’ properties, mechanisms of action, and potential human health benefits, researchers have used laboratory animals displaying pathophysiological changes specific to MetS. Polyphenols or their plant extracts were chosen according to the most advantageous mitigation of pathological changes in animal models best reflecting the components of MetS. The present paper comprises an overview of animal models of MetS, and promising polyphenolic compounds whose bioactive potential, effect on metabolic pathways, and supplementation-related benefits were analyzed based on in vivo animal models.

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Chronic resveratrol consumption prevents hypertension development altering electrophysiological currents and Ca2+ signaling in chromaffin cells from SHR rats

Cited by 11 publications

References 70 publications

PMCA Ca ²⁺ clearance in dental enamel cells depends on the magnitude of cytosolic Ca ²⁺

PMCA Ca ²⁺ clearance in dental enamel cells depends on the magnitude of cytosolic Ca ²⁺

Role of Resveratrol in Prevention and Control of Cardiovascular Disorders and Cardiovascular Complications Related to Covid-19 Disease: Mode of Action and Approaches Explored to Increase Its Bioavailability

Biological Potential of Polyphenols in the Context of Metabolic Syndrome: An Analysis of Studies on Animal Models

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Chronic resveratrol consumption prevents hypertension development altering electrophysiological currents and Ca2+ signaling in chromaffin cells from SHR rats

Cited by 11 publications

References 70 publications

PMCA Ca 2+ clearance in dental enamel cells depends on the magnitude of cytosolic Ca 2+

PMCA Ca 2+ clearance in dental enamel cells depends on the magnitude of cytosolic Ca 2+

Role of Resveratrol in Prevention and Control of Cardiovascular Disorders and Cardiovascular Complications Related to Covid-19 Disease: Mode of Action and Approaches Explored to Increase Its Bioavailability

Biological Potential of Polyphenols in the Context of Metabolic Syndrome: An Analysis of Studies on Animal Models

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PMCA Ca ²⁺ clearance in dental enamel cells depends on the magnitude of cytosolic Ca ²⁺

PMCA Ca ²⁺ clearance in dental enamel cells depends on the magnitude of cytosolic Ca ²⁺