Resveratrol enhances brown adipocyte formation and function by activating AMP‐activated protein kinase (AMPK) α1 in mice fed high‐fat diet

Abstract

Scope Enhancing the formation and function of brown adipose tissue (BAT) increases thermogenesis and hence reduces obesity. Thus, we investigate the effects of resveratrol (Resv) on brown adipocyte formation and function in mouse interscapular BAT (iBAT). Methods and results CD1 mice and stromal vascular cells (SVCs) isolated from iBAT were treated with Resv. Expression of brown adipogenic and thermogenic markers, and involvement of AMP-activated protein kinase (AMPK)α1 were assessed. In vivo, Resv enhanced expression of brown adipogenic markers, PR domain-containing 16 (PRDM16) and thermogenic genes, uncoupling protein 1 (UCP1) and cytochrome C in iBAT, along with smaller lipid droplets, elevated AMPKα activity and increased oxygen consumption. Meanwhile, Resv promoted expression of PRDM16, UCP1, PGC1α, cytochrome C and pyruvate dehydrogenase (PDH) in differentiated iBAT SVCs, suggesting that Resv enhanced brown adipocyte formation and function in vitro. In addition, Resv stimulated AMPKα and oxygen consumption in differentiated iBAT SVCs. However, the promotional effects of Resv were diminished by AMPK inhibition or AMPKα1 knockout, implying the involvement of AMPKα1 in this process. Conclusion Resv enhanced brown adipocyte formation and thermogenic function in mouse iBAT by promoting the expression of brown adipogenic markers via activating AMPKα1, which contributed to the anti-obesity effects of Resv.

Dietary apple polyphenols promote fat browning in high‐fat diet‐induced obese mice through activation of adenosine monophosphate‐activated protein kinase α

“…The differentiation and thermogenesis of classical brown and inducible beige/brite adipocytes are controlled by transcriptional regulators and signaling pathways . AMPK, an energy sensor that maintain energy homeostasis, is involved in the polyphenols‐mediated activation of brown fat and browning of WAT . Consistently, in this study, APs promote BAT activity and IngWAT browning in vivo through, at least partly, activation of AMPK α .…”

Programming of the Beige Phenotype in White Adipose Tissue of Adult Mice by Mild Resveratrol and Nicotinamide Riboside Supplementations in Early Postnatal Life

“…RSV exposure promotes mitochondrial activity in white and brown adipocyte cell models, and both WAT browning and activation of BAT thermogenesis have been implicated in body fat loss after RSV supplementation to adult rodents. In our study, early postnatal RSV supplementation favored the expression of brown adipocyte marker genes in iWAT of adult male mice but not in their BAT, where if anything there was decreased expression.…”

Antiobesity effects of resveratrol: which tissues are involved?

“…More recently, Wang et al . observed a decrease in the average daily weight gain and morphological changes in interscapular BAT (iBAT) in mice treated with resveratrol (0.1% w/w in a high‐fat diet) for 4 weeks.…”

“…Furthermore, the authors observed an increase, both in UCP1 gene expression and in protein expression of PRDM16 and cytochrome c , master factors involved in brown adipocyte formation, in the resveratrol‐ supplemented animals. They concluded that the enhanced formation and function of iBAT in mice fed the high‐fat diet and treated with resveratrol were associated with AMPK activation …”