Resveratrol Attenuates Benzo(a)pyrene-Induced Dysfunctions, Oxidative Stress and Apoptosis in Pancreatic Beta-Cells

Abstract

Background: Diabetes mellitus is one of the major health problems for people all over the world today. According to international diabetes federation reports, diabetes affects 382 million people worldwide. Environmental pollutants have deleterious effects on glucose metabolism and cause insulin resistance. We aimed to investigate the effects of the environmental pollutants benzo(a)pyrene, and the therapeutic potential of resveratrol. Methods: 20 µM of benzo(a)pyrene was administered after 48 h of resveratrol (80 µM) application for 24 h in INS-1 (832/13) insulinoma cells. The cells were treated with 20 μM benzo(a)pyrene for 24 hours after 48 hours initial preconditions with 10 μM resveratrol. Oxidative stress status, insulin secretion and apoptosis were analyzed by molecular techniques. Results: Though resveratrol increased the antioxidant status which was decreased by benzo(a)pyrene, interestingly, it increased the oxidative status. Resveratrol increased benzo(a)pyrenedepleted reduced glutathione levels to the control level. The mRNA expression levels of beta-cell functions associated with genes insulin-1, insulin-2 and sirtuin-1 were upregulated by resveratrol. Resveratrol treatment elevated the insulin concentration of culture medium, and the mRNA expression of forkhead box protein-1 gene. Resveratrol upregulated benzo(a)pyrene-downregulated p53 gene expression. On the other hand, benzo(a)pyrene-downregulated mRNA expression of B-cell lymphoma-2 was induced by resveratrol treatment. Conclusion: The data showed that resveratrol could reverse the oxidative alterations, functional impairments and the carcinogenetic effects of benzo(a)pyrene in pancreas beta-cells.

In Vitro Interaction of 5-Aminoorotic Acid and Its Gallium(III) Complex with Superoxide Radical, Generated by Two Model Systems

“…The antioxidant capacity by way of hydrogen donation is often estimated by measuring the radical-scavenging activity (RSA) toward the stable 2,2-diphenyl-1-picrylhydrazy radical (DPPH • ) [46][47][48], whereas participation in electron-exchange reactions may be tested by way of the ABTS •+ assay [49][50][51][52].…”

Molecular Mechanisms of Action of Selected Substances Involved in the Reduction of Benzo[a]pyrene-Induced Oxidative Stress

“…These data suggested amino acid and fatty acid metabolism regulated by AHR and PPAR-FATP1 signaling as potential therapeutic targets for intervening BaP-induced toxicity and related diseases. Also, Çelik et al [57] showed that resveratrol could reverse oxidative alterations induced by BaP. INS-1 (832/13) insulinoma cells were treated with BaP 20 at µM for 24 h after 48 h of initial preconditioning with resveratrol at 10 µM.…”

“…Resveratrol increases the level of amino acid and decreases the level of fatty acid. Also, Çelik et al [57] showed that resveratrol could reverse oxidative alterations induced by BaP. INS-1 (832/13) insulinoma cells were treated with BaP 20 at µM for 24 h after 48 h of initial preconditioning with resveratrol at 10 µM.…”