Resveratrol and curcumin as protective agents in an experimental rat model of intestinal ischemia and reperfusion

Abstract

The aim of this study was to evaluate the protective effects of resveratrol and curcumin in an experimental rat model of intestinal ischemia-reperfusion (I/R). 48 adult Wistar rats were used: 12 animals undergoing the sham surgery and 36 animals undergoing laparotomy, with 15 minutes of mesentric artery clamping. The animals from the latter groups (n=12) were pre-treated, for a week, with vehicle (CTR), resveratrol (RES) and curcumin (CUR). After one and 6 hours of reperfusion respectively, cyclooxigenase (COX)-2, mucin-1, E-cadherin, nuclear factor (NK)-kB expressions and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were assessed in the small intestine. Oxidative stress markers were determined in tissue homogenate and serum and histopathological analysis was performed.Pretreatment with RES decreased the expression of COX-2 and NF-kB at both intervals and increased E-cadherine (p<0.05) and mucin-1 production, after one hour. CUR had a beneficial effect on COX-2, NF-kB and E-cadherine expressions, both after one and 6 hours (p<0.0001). The two compounds increased TRAIL levels and had a protective effect on oxidative stress and histopatological lesions, both after one and 6 hours. Our results suggested that RES and CUR had beneficial effects in intestinal ischemia and may represent a promising option for complementary treatment of this pathological condition.

Resveratrol Improves Intestinal Morphology and Anti-Oxidation Ability in Deoxynivalenol-Challenged Piglets

“…Resveratrol (RES) is a naturally occurring polyphenol found in peanuts, red wine, grapes, pistachios, mulberries, and chocolate [9][10][11]. It has been shown that RES possesses efficient antioxidant activities both in vitro [12] and in vivo [13]. Supplementation with RES could significantly reverse the decline in the growth performance of piglets, and improve the feed efficiency of intrauterine growth-retarded suckling piglets [14].…”

“…Hence, the levels of DAO and D-lactic acid in plasma usually reflect the degree of damage to the intestinal barrier, also called intestinal permeability. Moreover, it has been suggested that the addition of RES could protect the intestinal mucosal-epithelial barrier in an ischemia/reperfusion rat model because of its antioxidant capacity [13,18]. Nevertheless, information regarding the effect of RES on intestinal injuries induced by DON in piglets is unavailable.…”

Effect of Resveratrol and Querceti̇n on Intesti̇nal Ischemi̇a Reperfusi̇on Injury i̇n Rats

“…In the study of Tanyeli et al 2 , it was observed that intestinal tissue TAS levels decreased significantly and TOS and OSI levels increased significantly in the rat group with mesenteric ischemia-reperfusion injury compared to the sham group. It has been seen in many studies that resveratrol has beneficial effects against intestinal I/R damage 14,[22][23][24][25] . In the study of Yıldız et al 12 , they found that TAS was significantly higher, TOS and OSI were significantly lower in the intestinal tissue in the resveratrol treatment group compared to the control group in intestinal I/R damage.…”

Protective effects of curcumin on ischemia/reperfusion injury

“…In a comparative study performed on a rat model of intestinal I/R, curcumin maintained significantly higher levels of E‐cadherin than resveratrol and for a longer period. The effect of curcumin on reducing OS (by lowering MDA levels and increasing glutathione (GSH): glutathione disulfide (GSSG) in intestinal tissue) as well as inhibiting inflammation (by reducing cyclooxygenase‐2 [COX‐2] inflammatory marker expression and increasing tumor necrosis factor‐related apoptosis‐inducing ligand [TRAIL] expression) was earlier and more persistent than resveratrol (Cucolas et al, 2016). Given the opposite effects of TRAIL on apoptosis induction, its overexpression appears to cause the shedding of the ischemia‐affected mature epithelial layer.…”