Resveratrol Ameliorates Experimental Alcoholic Liver Disease by Modulating Oxidative Stress

Abstract

The aim of this study was to investigate the hepatoprotective effects of resveratrol in alcoholic liver disease (ALD). Alcohol was administered to healthy female rats starting from 6% (v/v) and gradually increased to 20% (v/v) by the fifth week. After 16 weeks of intervention, liver enzymes (aspartate aminotransferase [AST] and alanine aminotransferase [ALT]) were analyzed using a chemistry analyzer, while hepatic antioxidant enzymes, oxidative stress markers, and caspase 3 activity were assessed using ELISA kits. Furthermore, hepatic CYP2E1 protein levels and mRNA levels of antioxidant and inflammation-related genes were determined using western blotting and RT-PCR, respectively. The results showed that resveratrol significantly attenuated alcohol-induced elevation of liver enzymes and improved hepatic antioxidant enzymes. Resveratrol also attenuated alcohol-induced CYP2E1 increase, oxidative stress, and apoptosis (caspase 3 activity). Moreover, genes associated with oxidative stress and inflammation were regulated by resveratrol supplementation. Taken together, the results suggested that resveratrol alleviated ALD through regulation of oxidative stress, apoptosis, and inflammation, which was mediated at the transcriptional level. The data suggests that resveratrol is a promising natural therapeutic agent against chronic ALD.

Natural compounds in the chemoprevention of alcoholic liver disease

“…Resveratrol significantly reverses the decreased activities of antioxidant CAT and glutathione peroxidase (GPx). The authors also found that resveratrol also improves apoptosis and inflammation in ethanol-treated rats (He et al, 2017).…”

Translational Approaches with Antioxidant Phytochemicals against Alcohol-Mediated Oxidative Stress, Gut Dysbiosis, Intestinal Barrier Dysfunction, and Fatty Liver Disease

“…Additionally, these beneficial agents can alter the metabolism and production of many endogenous compounds such as ethanol, acetaldehyde, SCFAs, bile acids, and trimethylamine (TMA) by altering the compositions of the gut microbiome [ 202 , 221 ] or perhaps even ethanol and acetaldehyde, which have been shown to be produced by some gut bacterial strains [ 202 , 218 , 219 , 220 , 222 ]. These antioxidant phytochemicals and/or their gut metabolites can also suppress the increased oxidative and nitrosative/nitrative stress by inhibiting the enzymes, such as CYP2E1, NOXs, iNOS, and NF-κB [ 223 , 224 , 225 ], as listed in the Figure. Furthermore, these beneficial antioxidant compounds from dietary supplements can affect key enzymes/proteins in the cellular signaling pathways, such as AMPK [ 226 ] and hepatic Sirt-1 [ 227 ], or transcription factors, including NF-κB and PGC-1α [ 228 , 229 ], to show their biological effects, as demonstrated by polyphenols in green and black teas [ 230 ].…”

Health Benefits of Resveratrol in Kidney Disease: Evidence from In Vitro and In Vivo Studies

“…RSV is found in the skin of grapes, in berries, and peanuts, with considerably high levels in grape juice (0.19–0.96 mg/L), and red wine (1.9 ± 1.7 mg/L) [43,44,45]. RSV has been studied for its pharmacological effects, including antioxidant, anti-inflammatory, immunomodulatory, hepatoprotective, anti-cancer, anti-atherosclerotic, and anti-diabetic properties [42,46,47,48,49,50,51].…”