Results of a Zika Virus (ZIKV) Immunoglobulin M–Specific Diagnostic Assay Are Highly Correlated With Detection of Neutralizing Anti-ZIKV Antibodies in Neonates With Congenital Disease

Cordeiro, Marli Tenório; Brito, Carlos; Pena, Lindomar; Castanha, Priscila M. S.; Gil, Laura Helena Vega Gonzales; Lopes, Kennya G. S.; Dhália, Rafael; Meneses, Jucille; Ishigami, Ana Catarina; Mello, Luisa Medeiros de; Alencar, Liciana Xavier Eurico de; Guarines, Klarissa Miranda; Rodrigues, Laura C.; Marques, Ernesto T. A.

doi:10.1093/infdis/jiw477

Cited by 55 publications

(53 citation statements)

References 20 publications

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“…The Panbio Dengue Capture ELISA was used for the anti-DENV IgM and IgG assays following the manufacturer’s protocol. Anti-Zika IgM was determined with the Centers for Diseases Control and Prevention protocol (28, 51). Samples were classified as Zika virus–positive if they were positive for Zika virus RNA by qRT-PCR in the acute phase and positive for anti–Zika virus IgM with titers more than two times those for anti-DENV IgM in the convalescent phase when the convalescent sample was available (37 of 49 patients).…”

Section: Methodsmentioning

confidence: 99%

Rapid and specific detection of Asian- and African-lineage Zika viruses

et al. 2017

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Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.

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Section: Methodsmentioning

confidence: 99%

Rapid and specific detection of Asian- and African-lineage Zika viruses

et al. 2017

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“…117,118 ELISA testing of neonatal sera and cerebrospinal fluid for ZIKV-specific IgM has confirmed congenital ZIKV infections in newborns with microcephaly. 119 Most recently, a reverse transcription loop mediated isothermal amplification method for detecting ZIKV has been reported as a more rapid and field-safe test for ZIKV infection. 120 useful for studying vertical transmission of ZIKV as pups born to ZIKV-infected mothers have Zika virons throughout their bodies and concentrated in their brains.…”

Section: Zikv Detection and Diagnosismentioning

confidence: 99%

Potential mechanisms of Zika‐linked microcephaly

Merfeld

Ben-Avi

Kennon

et al. 2017

WIREs Developmental Biology

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A recent outbreak of Zika virus (ZIKV) in Brazil is associated with microcephaly in infants born of infected mothers. As this pandemic spreads, rapid scientific investigation is shedding new light on how prenatal infection with ZIKV causes microcephaly. In this analysis we provide an overview of both microcephaly and ZIKV, explore the connection between prenatal ZIKV infection and microcephaly, and highlight recent insights into how prenatal ZIKV infection depletes the pool of neural progenitors in the developing brain. WIREs Dev Biol 2017, 6:e273. doi: 10.1002/wdev.273For further resources related to this article, please visit the WIREs website.

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“…All samples tested positive and negative for anti-DENV IgG and IgM, respectively (anti-DENV IgG indirect ELISA and IgM capture ELISA; PanBio). Pooled sera from individuals who had experienced primary ZIKV infection were collected in epidemiological studies conducted in Brazil (3,60). Sera were confirmed to have neutralizing antibodies against ZIKV but no other DENV serotype by plaque reduction neutralization assay.…”

Section: Methodsmentioning

confidence: 99%