Results of a study of genetic factors predisposing to the development of psoriasis among the population of the Russian Federation

Abstract

Goal. To assess the incidence of genes predisposing to the development of psoriasis based on selected molecular and genetic study methods among the Russian population. Materials and methods. By using allele specific hybridization methods in the form of real-time PCR and RFLP assay (Restriction Fragment Length Polymorphism), single-nucleotide polymorphisms of fragments of 13 genes associated with predisposition to the development of psoriasis were studied in whole blood samples obtained from 45 psoriatic patients: rs4649203 (gene IL-28RA), rs11209026 (gene IL-23R), rs610604 (gene TNFAIP3), rs514315 (gene SERPINB8), rs9304742 (gene ZNF816A), rs17728338 (gene TNIP1), rs13190932 (gene TRAF3IP2), rs2235617 (gene ZNF313), rs27524 (gene ERAP1), rs702873 (gene REL), rs3213094 (gene IL-12B), rs12720356 (gene TYK2), and rs8016947 (gene NFKBI). Results. A set of data on the genotype structure and incidence in respective loci of 13 genes associated with predisposition to develop psoriasis was obtained for the first time based on a Russian sample of patients. At this stage of the study, we failed to reveal any reliable differences concerning the incidence of individual genotypes between psoriatic patients and healthy people because of the small size of the sample. Gene TYK2 (rs1272035) served as an exclusion. The homozygous T/T genotype was revealed more often (p < 0.01) in psoriatic patients (95.6%) vs. healthy people (77.8%), which makes it possible to consider the carriers of this genotype as people predisposed to the development of psoriasis.