Phenotypic methods were initially used for bacterial typing yet they have a number of drawbacks limiting their use. Methods of molecular and genetic typing have become wide-spread today. Among these methods, bacterial typing based on multilocus sequence
typing (Multilocus Sequence Typing - MLST) has been developing at the fastest rate. However, schemes of molecular
and genetic typing of STD pathogens as compared to other bacteria are insufficiently developed, which considerably complicates
the planning of measures aimed at the reduction of their spread.
The article is devoted to the development and validation of a standard operating procedure entitled «Molecular typing
of С. trachomatis for assessment of the clonal structure and extent of genetic diversity of С. trachomatis strains
circulating in the territory of the Russian Federation.» The studies that involved the typing of C. trachomatis strains
obtained from regions of the Russian Federation with the use of six c. trachomatis genes (ompA, СТ046, cT058, cT144,
cT172 and pbpB) revealed their significant heterogenicity with the prevalence of the E serotype. However, the typing of
c. trachomatis for six genes at one time seems to be too time-consuming and long-lasting and is hard to be interpreted,
which explains the need to develop other typing protocols using a smaller number of relevant genes (not more than two
or three - by analogy with NG-MAST).
Represents results of research, dedicated to the search of genetically determined factors of С. trachomatis virulence. Data of papers, studying features of С. trachomatis genetic variations was highlighted.
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