Restriction Fragment Length Polymorphisms of Grapevine DNA with Phenylalanine Ammonia-Lyase cDNA.

Yamamoto, Naokatsu; Ono, Genki; Takashima, Kunio; Yotsuka, Akira

doi:10.1270/jsbbs1951.41.365

Cited by 14 publications

(3 citation statements)

References 6 publications

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“…Although some workers have described potential difficulties in using RAPD markers (Ellsworth et al, 1993;Heun and Helentjaris, 1993;Penner et al, 1993;Riedy et al, 1992;Weeden et al, 1992), the present study was undertaken to evaluate their utility for fingerprinting grape (Vitis) rootstocks. Restriction fragment-length polymorphism (RFLP) analyses have revealed genetic differences among grape rootstocks (Bourquin et al, 1991) and cultivars (Bowers et al, 1993;Mauro et al, 1992;Sivolap et al, 1992;Yamamoto et al, 1991), but the differences are not yet detectable in a PCR-based assay. In contrast, sequences.…”

mentioning

confidence: 99%

Sequence-specific Polymerase Chain-reaction Markers Derived from Randomly Amplified Polymorphic DNA Markers for Fingerprinting Grape (Vitis) Rootstocks

Xu¹,

Wilson²,

Arulsekar³

et al. 1995

jashs

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Additional index words. sequence characterized amplified region, SCAR Abstract. Randomly amplified polymorphic DNA (RAPD) markers were generated for identifying grape (Vitis) rootstocks. Seventy-seven primers (10 bases long) were screened using CsCl-purified leaf DNA derived from several field samples of nine rootstocks sampled in successive years. Nine RAPD markers were detected from six primers and, in combination, distinguished all nine rootstocks tested. Because inconsistencies were encountered in performing the RAPD assay, sequence-specific primers were derived from cloned RAPD bands for use under more stringent amplification conditions. Southern hybridization analysis of the RAPD gels with cloned RAPD bands as probes revealed deficiencies of scoring RAPD bands based solely on ethidium bromide staining. In some cases, bands of the same size generated by the same primer in different rootstocks-normally scored as the same marker-failed to cross-hybridize, implying lack of homology between the bands. More commonly, bands scored as absent based on ethidium bromide staining were detected by hybridization. Six of the nine cloned RAPD bands were partially sequenced, and sequence-specific primer pairs were synthesized. Two primer pairs amplified a product the same size as the original RAPD band in all rootstocks, resulting in loss of polymorphism. Two other pairs of sequence-specific primers derived from the same marker failed to amplify the expected band consistently. Three of the most useful primer pairs amplified apparent length variants in some accessions and will have value as polymerase chain-reaction markers for fingerprinting.

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mentioning

confidence: 99%

Sequence-specific Polymerase Chain-reaction Markers Derived from Randomly Amplified Polymorphic DNA Markers for Fingerprinting Grape (Vitis) Rootstocks

Xu¹,

Wilson²,

Arulsekar³

et al. 1995

jashs

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show abstract

“…Genomic DNA was extracted from 2 g of young leaf based on the method of Couch and Fritz (1990) modified by Yamamoto et al (1991). After RNase treatment, DNA was purified using a DNeasy Plant Mini Kit (Qiagen, USA), as described in the manufacturer's manual.…”

Section: Dna Extraction Labeling and Genechips Hybridizationmentioning

confidence: 99%

Expression analysis of UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene in an interspecific hybrid grape between Vitis ficifolia var. ganebu and Vitis vinifera cv. Muscat of Alexandria

Poudel

Goto‐Yamamoto

Mochioka

et al. 2008

Plant Biotechnol Rep

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“…Recently, multiple simply inherited markers such as allozyme composition (Wolfe, 1976 ;Schwennesen et al ., 1982 ;Loukas et al, 1983 ;Goldy et al ., 1988 ;Weeden et al ., 1988 ;Chaparro et al, 1989 ;Durham et al, 1989; and DNA restriction sites (Yamamoto et al, 1991) have been developed in grape as well as in many other crops (Weeden, 1989) . In grape, isozyme techniques have been useful for cultivar identification (Wolfe, 1976 ;Schwennesen et al ., 1982 ;Parfitt, & Arulsekar, 1989) and hybridity confirmation (Goldy et al ., 1988 ;Chaparro et al ., 1989 ;Durham et al, 1989 ;Goldy et al, 1989), and 17 polymorphic loci including Gpi-2 and Pgm-2 have been defined Weeden et al, 1988) .…”

Section: Introductionmentioning

confidence: 99%

Study of the parentage of grape cultivars by genetic interpretation of GPI-2 and PGM-2 isozymes

1992

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Restriction Fragment Length Polymorphisms of Grapevine DNA with Phenylalanine Ammonia-Lyase cDNA.

Cited by 14 publications

References 6 publications

Sequence-specific Polymerase Chain-reaction Markers Derived from Randomly Amplified Polymorphic DNA Markers for Fingerprinting Grape (Vitis) Rootstocks

Sequence-specific Polymerase Chain-reaction Markers Derived from Randomly Amplified Polymorphic DNA Markers for Fingerprinting Grape (Vitis) Rootstocks

Expression analysis of UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) gene in an interspecific hybrid grape between Vitis ficifolia var. ganebu and Vitis vinifera cv. Muscat of Alexandria

Study of the parentage of grape cultivars by genetic interpretation of GPI-2 and PGM-2 isozymes

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