Study of the parentage of grape cultivars by genetic interpretation of GPI-2 and PGM-2 isozymes

Ohmi, Chie; Wakana, Akira; Shiraishi, Shinichi

doi:10.1007/bf00023083

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…In practice, however, selfed progeny tend to be non-viable, presumably due to a high deleterious recessive load and resulting inbreeding depression. As a consequence of these fitness effects, most grape cultivars are crosses between distantly related parents (Strefeler et al, 1992 ; Ohmi et al, 1993 ; Bowers and Meredith, 1997 ; Sefc et al, 1998 ; Lopes et al, 1999 ; Di Gaspero et al, 2005 ; Tapia et al, 2007 ; Ibáñez et al, 2009 ; Cipriani et al, 2010 ; Myles et al, 2011 ; Lacombe et al, 2013 ).…”

Section: High Heterozygosity Is a Challenge For Grape Genome Assemblymentioning

confidence: 99%

“…Whether a spontaneous hybrid or a product of human breeding, all of the Cabernet Sauvignon grown around the world is thought to have resulted from this single hybridization event. Just as the parents of Cabernet Sauvignon have been identified, the genetic origin of many other important wine grape cultivars is known, and they often originate from the direct crossing of common, distantly-related cultivars (Strefeler et al, 1992 ; Ohmi et al, 1993 ; Qu et al, 1996 ; Bowers and Meredith, 1997 ; Sefc et al, 1998 ; Lopes et al, 1999 ; Crespan and Milani, 2001 ; Vouillamoz et al, 2003 , 2004 ; Di Gaspero et al, 2005 ; Vouillamoz and Grando, 2006 ; Lacombe et al, 2007 , 2013 ; Tapia et al, 2007 ; Boursiquot et al, 2009 ; Ibáñez et al, 2009 ; Cipriani et al, 2010 ; Myles et al, 2011 ; García-Muñoz et al, 2012 ). Due to this intraspecific hybridization process, levels of heterozygosity in grape cultivars can easily exceed 11% (Jaillon et al, 2007 ).…”

Section: High Heterozygosity Is a Challenge For Grape Genome Assemblymentioning

confidence: 99%

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How Single Molecule Real-Time Sequencing and Haplotype Phasing Have Enabled Reference-Grade Diploid Genome Assembly of Wine Grapes

et al. 2017

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Section: High Heterozygosity Is a Challenge For Grape Genome Assemblymentioning

confidence: 99%

Section: High Heterozygosity Is a Challenge For Grape Genome Assemblymentioning

confidence: 99%

How Single Molecule Real-Time Sequencing and Haplotype Phasing Have Enabled Reference-Grade Diploid Genome Assembly of Wine Grapes

et al. 2017

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“…Although the V. vinifera genome is relatively small (∼487Mb) (Lodhi and Reisch 1995; Jaillon et al 2007) and as repetitive as other plant genomes of similar size (41.4%) (Jaillon et al 2007; Michael and Jackson 2013), it is highly heterozygous (∼13%) (Jaillon et al 2007; Velasco et al 2007). Most domesticated grape cultivars are crosses between distantly related parents; this and clonal propagation cause the high heterozygosity observed in the species (Strefeler et al 1992; Ohmi et al 1993; Bowers and Meredith 1997; Sefc et al 1998; Lopes et al 1999; Di Gaspero et al 2005; Tapia et al 2007; Ibáñez et al 2009; Cipriani et al 2010; Myles et al 2011; Lacombe et al 2013; Chin et al 2016; Minio et al 2017; Zhou et al 2017). Earlier attempts using short reads struggled to resolve complex, highly heterozygous genomes (Gnerre et al 2011; Huang et al 2012; Di Genova et al 2014; Kajitani et al 2014; Safonova et al 2015).…”

mentioning

confidence: 99%

Iso-Seq Allows Genome-Independent Transcriptome Profiling of Grape Berry Development

Minio

Massonnet

Figueroa‐Balderas

et al. 2019

G3 Genes|Genomes|Genetics

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Transcriptomics has been widely applied to study grape berry development. With few exceptions, transcriptomic studies in grape are performed using the available genome sequence, PN40024, as reference. However, differences in gene content among grape accessions, which contribute to phenotypic differences among cultivars, suggest that a single reference genome does not represent the species’ entire gene space. Though whole genome assembly and annotation can reveal the relatively unique or “private” gene space of any particular cultivar, transcriptome reconstruction is a more rapid, less costly, and less computationally intensive strategy to accomplish the same goal. In this study, we used single molecule-real time sequencing (SMRT) to sequence full-length cDNA (Iso-Seq) and reconstruct the transcriptome of Cabernet Sauvignon berries during berry ripening. In addition, short reads from ripening berries were used to error-correct low-expression isoforms and to profile isoform expression. By comparing the annotated gene space of Cabernet Sauvignon to other grape cultivars, we demonstrate that the transcriptome reference built with Iso-Seq data represents most of the expressed genes in the grape berries and includes 1,501 cultivar-specific genes. Iso-Seq produced transcriptome profiles similar to those obtained after mapping on a complete genome reference. Together, these results justify the application of Iso-Seq to identify cultivar-specific genes and build a comprehensive reference for transcriptional profiling that circumvents the necessity of a genome reference with its associated costs and computational weight.

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“…Traditional methods for fruit characterization recognition depend on the morphological properties which are also affected by environmental and growth factors, and subsequently decrease markers efficiency ( Ohmi et al, 1993). So, DNA molecular markers have been widely used to assess genetic diversity and germplasm characterization.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between the Use of Gallic Acid and Genetic Stability of Grapes

Elbotaty

Heiba

Saleh

2023

Egyptian Journal of Horticulture

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T HE effect of Gallic acid (GA) at 200, 300, and 350 mg L -1 , on the genetic stability of SO4 and Freedom micropropagated grape rootstocks was studied through two subcultures. Plantlets' vegetative parameters didn't differ significantly between both primary subcultures. An inverse correlation occurred between elevated GA concentration in culture medium and plantlets' vegetative growth, meanwhile, it was a promoter by a moderate concentration of 200 mg L -1 . Furthermore, when GA exceeded 350 mg L -1 caused plantlets' breakdown. Moreover, fingerprinting analysis, RAPD-PCR has been performed to investigate relationships among GA presences in culture media during subcultures and their genetic profile. It detected 93 monomorphic bands with a ratio of 73.8 % and 18 polymorphic bands with a ratio of 16.2 %, out of them, 11 in the 1 st subculture, while 7 in the 2 nd subculture were detected. Under GA concentrations with a total ratio of 16.2 %, the polymorphism ratio was 61.1 % in the 1 st subculture and decreased to 38.9 % in the 2 nd subculture using 350 mg L -1 of GA. Gallic acid concentrations, of 200, 300 and 350 mg L -1 in growth medium preserved both micro-propagated rootstocks' genetic stability through two subcultures.

show abstract

Study of the parentage of grape cultivars by genetic interpretation of GPI-2 and PGM-2 isozymes

Cited by 8 publications

References 18 publications

How Single Molecule Real-Time Sequencing and Haplotype Phasing Have Enabled Reference-Grade Diploid Genome Assembly of Wine Grapes

How Single Molecule Real-Time Sequencing and Haplotype Phasing Have Enabled Reference-Grade Diploid Genome Assembly of Wine Grapes

Iso-Seq Allows Genome-Independent Transcriptome Profiling of Grape Berry Development

Relationship Between the Use of Gallic Acid and Genetic Stability of Grapes

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