Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species

Fisher, L N; Mathiesen, Michelle R.; Duhamel, G

doi:10.1128/cdli.4.6.681-686.1997

Cited by 17 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The structural, biochemical and genotypic characteristics of B. pilosicoli isolated from animals are similar to those of B. pilosicoli isolated from humans (Lee and Hampson, 1994;Duhamel et al, 1995b;Trott et al, 1996dFisher et al, 1997;Muniappa et al, 1998). Transmission across species has been shown in pigs, chicks and laboratory mice inoculated with B. pilosicoli strains from humans, monkeys, pigs, dogs and birds (Dwars et al, 1992;Muniappa et al, 1996Muniappa et al, , 1998Trott et al, 1996c;Sacco et al, 1997;.…”

Section: Perspective and Future Directionssupporting

confidence: 55%

Comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis

Duhamel

2001

Anim. Health. Res. Rev.

View full text Add to dashboard Cite

Research in the past decade has led to the recognition of Brachyspira (formerly Serpulina) pilosicoli as the primary etiologic agent of colonic spirochetosis (CS), an emerging cause of colitis in humans and animals. Attachment of spirochetes to the epithelial surface of the lower intestine is considered to be the hallmark of CS. However, because B. pilosicoli, B. aalborgi and unclassified flagellated bacteria are found singly or together in humans and non-human primates with CS lesions, attachment of spiral-shaped bacteria may not represent the same etiopathogenetic entity in all hosts. Moreover, North American opossums with CS are infected with B. aalborgi-like spirochetes together with flagellated bacteria, whereas B. pilosicoli is found alone in dogs, pigs, chickens and other species of birds with CS. Conversely, guinea-pigs with CS have unidentified spirochetes that may be B. pilosicoli or B. aalborgi. The pig model of CS suggests that attachment of B. pilosicoli to epithelial cells may be transient. By contrast, persistence of B. pilosicoli in the cecal and colonic crypt lumina, chronic inflammation caused by spirochetal invasion into the subepithelial lamina propria and translocation to extraintestinal sites may be more important than previously thought. This review describes the lesions seen in naturally occurring and experimentally induced CS of animals, and it sets the stage for future research into the pathogenic mechanisms of infection and colitis caused by B. pilosicoli.

show abstract

Section: Perspective and Future Directionssupporting

confidence: 55%

Comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis

Duhamel

2001

Anim. Health. Res. Rev.

View full text Add to dashboard Cite

show abstract

“…serotyping based on reacting extracted lipooligosaccharide with hyperimmune rabbit sera (Baum and Joens, 1979), DNA restriction endonuclease analysis (REA) (Combs et al, 1992;Harel et al, 1994), random amplification of polymorphic DNA (RAPD) (Dougard et al, 1996), DNA restriction fragment polymorphism analysis (RFLP) (Fisher et al, 1997), and pulsed field gel electrophoresis (PFGE) (Atyeo et al, 1999;Fellström et al, 1999). In addition, multilocus enzyme electrophoresis (MLEE) has been used to examine the population structure and diversity of B. hyodysenteriae (Lee et al, 1993;Trott et al, 1997).…”

Section: B Hyodysenteriae Isolates Have Been Typed Using Various Metmentioning

confidence: 99%

Multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of Brachyspira hyodysenteriae

Phillips

Harland

et al. 2009

Veterinary Microbiology

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The purpose of this study was to develop and apply a multilocus sequence typing 27 (MLST) scheme to study the molecular epidemiology of Brachyspira hyodysenteriae, 28 the aetiological agent of swine dysentery. Sequences of seven conserved genomic loci 29 were examined in 111 B. hyodysenteriae strains. Fifty-eight of these previously had 30 been analysed by multilocus enzyme electrophoresis (MLEE), and for some the 31 results of pulsed field gel electrophoresis (PFGE), restriction endonuclease analysis 32 (REA) and/or serotyping also were available. The discriminatory power of these 33 methods was compared. The strains were divided into 67 sequence types (STs) and 46 34 amino acid types (AATs) by MLST. The Index of Association value was significantly 35 different from zero, indication that the population was clonal. Eleven clonal 36 complexes (Cc) comprising between 2 and 10 STs were recognised. A population 37 snapshot based on AATs placed 77.5% of the isolates from 30 of the AATs into one 38 major cluster. The founder type AAT9 included 13 strains from nine STs that were 39 isolated in Australia, Sweden, Germany and Belgium, including one from a mallard. 40The MLST results were generally comparable to those produced by MLEE. The 41 MLST system had a similar discriminatory power to PFGE, but was more 42 discriminatory than REA, MLEE or serotyping. MLST data provided evidence for 43 likely transmission of strains between farms, but also for the occurrence of temporal 44 "micro-evolution" of strains on individual farms. Overall, the MLST system proved to 45 be a useful new tool for investigating the molecular epidemiology and diversity of B. 46 hyodysenteriae. 47 48

show abstract

“…), DNA restriction fragment polymorphism analysis (Fisher et al . ), pulsed field gel electrophoresis (Atyeo et al . ) and multilocus enzyme electrophoresis (Lee et al .…”

Section: Introductionmentioning

confidence: 99%

Characterization ofBrachyspira hyodysenteriaeisolates from Italy by multilocus sequence typing and multiple locus variable number tandem repeat analysis

et al. 2017

View full text Add to dashboard Cite

show abstract

Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species

Cited by 17 publications

References 37 publications

Comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis

Comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis

Multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of Brachyspira hyodysenteriae

Characterization ofBrachyspira hyodysenteriaeisolates from Italy by multilocus sequence typing and multiple locus variable number tandem repeat analysis

Contact Info

Product

Resources

About