Restriction Fragment Length Polymorphism of Urease C and Urease B Genes of Helicobacter pylori Strains Isolated from Brazilian Patients with Peptic Ulcer and Chronic Gastritis

Röesler, Bruna Maria; Oliveira, Telma Barbosa de; Bonon, Sandra Helena Alves; Monici, Leonardo Trevizan; Zeitune, José Murilo Robilotta; Costa, Sandra Cecı́lia Botelho

doi:10.1007/s10620-008-0525-2

Cited by 7 publications

(4 citation statements)

References 27 publications

(20 reference statements)

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“…The strain-specific genes found in the comparison of sequenced strains are consistent with earlier studies that demonstrated the high diversity of the H. pylori genome [47–49]. Consequently, this high level of genetic diversity can be an important factor in its adaptation to the host stomach and for the clinical outcome of the infection although this aspect remains unclear.…”

Section: H Pylori Eradication and Prevention Of Gastric Cancersupporting

confidence: 87%

Eradication Treatment of Helicobacter pylori Infection: Its Importance and Possible Relationship in Preventing the Development of Gastric Cancer

Röesler

Costa

Zeitune

2012

ISRN Gastroenterology

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Helicobacter pyloriis the most important carcinogen for gastric adenocarcinoma. Bacterial virulence factors are essential players in modulating the immune response involved in the initiation of carcinogenesis in the stomach; host genetic factors contribute to the regulation of the inflammatory response and to the aggravation of mucosal damage. In terms of environmental factors, salt intake and smoking contribute to the development of lesions. Various therapeutic schemes are proposed to eradicateH. pyloriinfection, which could potentially prevent gastric cancer, offering the greatest benefit if performed before premalignant changes of the gastric mucosa have occurred.

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Section: H Pylori Eradication and Prevention Of Gastric Cancersupporting

confidence: 87%

Eradication Treatment of Helicobacter pylori Infection: Its Importance and Possible Relationship in Preventing the Development of Gastric Cancer

Röesler

Costa

Zeitune

2012

ISRN Gastroenterology

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“…PCR-RFLP has been frequently used for genotyping and discrimination of H. pylori strains because it is low cost, rapid, easy to perform and can be used to analyze H. pylori genotype diversity ( Röesler et al , 2009 ). The two restriction endonucleases Hha I and Mbo I were chosen for RFLP analysis of the ure C gene ( Li et al , 1997 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Helicobacter pylori in children in eastern Turkey and molecular typing of isolates

Ozbey¹,

Doğan²,

Demirören³

et al. 2015

Braz. J. Microbiol.

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The objectives of the present study were to determine Helicobacter pylori via culture, polymerase chain reaction and histopathological diagnosis in 101 children ranging in age from 4 to 18 years, to identify the association among restriction fragment length polymorphism types and clinical disease and to investigate the relationships among different isolates of H. pylori in different age groups. We observed a high prevalence of H. pylori infections in children between the ages of 13 and 18 (75.8%), while children aged 4 to 6 years had the lowest prevalence of infection (40%). H. pylori was detected in 30.7% (31 of 101), 66.3% (67 of 101) and 63.2% (60 of 95) of children as determined by culture methods, PCR and histological examination, respectively. H. pylori isolates with RFLP types I and III were the most common among children with antral nodularity, whereas RFLP types II and IV were the least detected types. Interestingly, all isolates from peptic ulcer patients were type III. Although our results show a high prevalence of H. pylori infections in the pediatric population in eastern Turkey, no association was identified between H. pylori infection with antral nodularity and recurring abdominal pain. In addition, we found low genetic variation among H. pylori isolates from children and no association between RFLP types and antral nodularity (p > 0.05). Additionally, we found that H. pylori isolates with specific RFLP types were predominant in different age groups.

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“…From Gastritis to Gastric Cancer: The Importance of CagPAI of Helicobacter Pylori on the Development of Early and Advanced Gastric Adenocarcinoma http://dx.doi.org/10.5772/53208 With respect specifically to the bacterium strains, H. pylori has a high level of genetic diversity that represents an important factor in its adaptation to the host stomach and also for the clinical outcome of the infection [32,33]. Virulence factors of Helicobacter pylori are essential players in modulating the immune response involved in the initiation of carcinogenesis in the stomach.…”

Section: Introductionmentioning

confidence: 99%

From Gastritis to Gastric Cancer: The Importance of CagPAI of Helicobacter Pylori on the Development of Early and Advanced Gastric Adenocarcinoma

Röesler¹,

Zeitune²

2013

Current Topics in Gastritis - 2012

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Restriction Fragment Length Polymorphism of Urease C and Urease B Genes of Helicobacter pylori Strains Isolated from Brazilian Patients with Peptic Ulcer and Chronic Gastritis

Cited by 7 publications

References 27 publications

Eradication Treatment of Helicobacter pylori Infection: Its Importance and Possible Relationship in Preventing the Development of Gastric Cancer

Eradication Treatment of Helicobacter pylori Infection: Its Importance and Possible Relationship in Preventing the Development of Gastric Cancer

Prevalence of Helicobacter pylori in children in eastern Turkey and molecular typing of isolates

From Gastritis to Gastric Cancer: The Importance of CagPAI of Helicobacter Pylori on the Development of Early and Advanced Gastric Adenocarcinoma

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