Restriction fragment length polymorphism analysis of the VP2 gene of Australian strains of infectious bursal disease virus

Sapats, S; Ignjatovic, Jagoda

doi:10.1080/0307945021000024625

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…The presence or absence of the SspI site was not useful in predicting a vv pathotype since, when present, it was located at a different position than in vvIBDVs. This finding was also reported for Australian IBDVs (Sapats & Ignjatovic, 2002). Antigenic variation of IBDVs is largely due to mutations occurring in two main hydrophilic regions of VP2, located between amino acid residues 212 to 223 and amino acid residues 314 to 324 (Vakharia et al, 1994).…”

Section: Discussionsupporting

confidence: 68%

Genotyping of Canadian field strains of infectious bursal disease virus

et al. 2007

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For this retrospective study, infectious bursal disease virus (IBDV) was detected in 134 bursal samples that originated from flocks with conditions such as airsacculitis, tracheitis, pneumonia, septicaemia, inclusion body hepatitis, coccidiosis, and/or a history of production problems without overt clinical symptoms. Samples were from seven Canadian provinces: Ontario, Quebec, Manitoba, British Columbia, Nova Scotia, Alberta, and Newfoundland and Labrador. Viral RNA was identified in bursae with moderate to severe and acute to chronic bursal damage. The ages of the flocks from which samples were collected ranged from 3 to 63 days. Following reverse transcriptase-polymerase chain reaction the nucleotide sequence of the VP2 hypervariable region was determined and compared with sequences available in GenBank. The most common Canadian IBDV field strains were North-American variant viruses. Forty-four viruses were highly related (97.5% to 100.0%) to the US IBDV strain NC171. Moreover, 16 field viruses whose VP2 sequences were 99.2% to 100% identical to the South African 05SA8 IBDV strain appeared closely related to the NC171 group. Delaware E-related field viruses, 98.3% to 100.0% identical to the prototype virus, were identified in 33 samples. Thirty-four Canadian IBDVs showed the highest identity, 94.2% to 98.3%, to US IBDV strain 586. Five samples contained vaccine-related viruses, while two field strains showed the best match to Del A (United States) and IBDV strains SP_04_02 (Spain). Very virulent IBDVs were not detected in Canada.

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Section: Discussionsupporting

confidence: 68%

Genotyping of Canadian field strains of infectious bursal disease virus

et al. 2007

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“…The purified FAdVs were tested by PCR or by reverse transcription-PCR (RT-PCR) to confirm the absence of known avian pathogens, including avian reovirus (Bruhn et al, 2005), chicken anaemia virus (Kaffashi et al, 2006), infectious bursal disease virus (Sapats & Ignjatovic, 2002), avian encephalomyelitis virus (Marvil et al, 1999;Xie et al, 2005), avian leukosis virus (Hauptli et al, 1997;Bagust et al, 2004;Fenton et al, 2005), Marek's disease virus (Zhu et al, 1992), reticuloendotheliosis virus (Aly et al, 1993), egg drop syndrome virus (Raj et al, 2001), Mycoplasma gallisepticum (Ghorashi et al, 2010), Mycoplasma synoviae (Jeffery et al, 2007) and Chlamydia/Chlamydophila species (Robertson et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens

et al. 2015

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Fowl adenoviruses (FAdVs) cause diseases in domestic chickens, including inclusion body hepatitis (IBH), with immunosuppression believed to play a role in their pathogenesis. To gain a better understanding of the pathogenesis and chronology of disease caused by FAdVs, the gross pathology, histopathology and dissemination of virus were examined at several different time points, after inoculation of one-day-old specific pathogen-free chickens with FAdV-1, FAdV-8b or FAdV-11 via the ocular route. FAdV-8b had a slightly greater virulence than FAdV-11, but both were primary pathogens. The presence and severity of hepatic lesions were used to define the three stages of the disease: incubation (1-3 days post-inoculation, PI), degeneration (4-7 days PI) and convalescence (14 days PI). Both viruses were detected in the liver, kidney, bursa, thymus and gizzard of most birds during the degenerative stage, and persisted in the gizzard into convalescence. The FAdV-1 isolate was found to be apathogenic, but virus was detected in the bursa and/or gizzard of several birds between 2 and 7 days PI. This is the first study examining the chronology of gross and microscopic lesions of pathogenic and apathogenic FAdVs in association with viral presence in multiple tissues. It was concluded that both FAdV-8b and FAdV-11 are primary pathogens, and that these strains may play a role in immunosuppression.

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“…PCR was performed on seven samples: Bukuru outbreak, n=3, Vom outbreak, n=1, and Vaccine strains, n=3. PCR was carried out using primers J1: 5′-GGC CCA GAG TCT ACA CCA TAA C-3′ and J2: 5′-CCG GAT TAT GTC TTT GAA GCC-3′ (Jackwood and Sommer 1999;Sapats and Ignjatovic 2002). Clear and distinct bands of about 743 bp were considered positive.…”

Section: Methodsmentioning

confidence: 99%

Further evidence for very virulent infectious bursal disease virus in vaccinated chickens in Nigeria

Owolodun

Yakubu

Jambol

et al. 2015

Trop Anim Health Prod

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Reverse transcription polymerase chain reaction (RT-PCR) and partial sequencing of the VP2 hypervariable region was performed on clinical samples from two infectious bursal disease (IBD) outbreaks in Plateau state, Nigeria. IBD virus RNA was detected in all four bursa of Fabricius samples. Nucleotide sequencing and analysis of the four samples revealed high similarity to previous IBDV sequences from northern and southern Nigeria. The deduced amino acid sequences were compared to reference IBDV strains retrieved from the GenBank; virulence markers A222, I256, and I294 were conserved in both outbreak and reference sequences. Amino acid residue S254 was conserved in the outbreak viruses and previous viruses from northern Nigeria. Phylogenetic analysis revealed that all four viruses were very virulent IBDVs. These viruses clustered with vv2-1 variant viruses from Oyo and Ogun states and less closely with vv2-2 isolates from Tanzania. The nucleotide identity of the sequences in this study ranged from 99.6 to 100 % with each other. These findings are further evidence of IBD outbreaks in vaccinated chicken flocks in Nigeria.

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Restriction fragment length polymorphism analysis of the VP2 gene of Australian strains of infectious bursal disease virus

Cited by 15 publications

References 34 publications

Genotyping of Canadian field strains of infectious bursal disease virus

Genotyping of Canadian field strains of infectious bursal disease virus

Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens

Further evidence for very virulent infectious bursal disease virus in vaccinated chickens in Nigeria

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