Restriction enzyme analysis of faecal adenoviruses in Newcastle upon Tyne

Abstract

SUMMARYAdenovirus DNA was isolated directly from virus-containing stools and digested with restriction endonucleases. The resulting fragments were separated by polyacrylamide gel electrophoresis (PAGE) and visualized by silver staining. This enabled us to assign most of the viruses detected to subgenus, serotype and, sometimes, unique strains. Although less sensitive than electron microscopy, the method allowed more information about the infecting virus to be obtained and no cultivation was necessary. Comparison with culture also allowed dual infections to be recognized.A 2-year survey of faecal adenoviruses in Newcastle upon Tyne showed that type 41 (strain 41a) was the predominant type and strain 41p was not recorded. Heterogeneity in strain 41a was also noted as found elsewhere. Adenovirus type 40 was common prior to 1985 but was absent during the last 2 years.

Adenoviruses isolated in the Stockholm area during 1987?1992: restriction endonuclease analysis and molecular epidemiology

“…Also nine of ten Ad41 isolates sent to us from another Swedish laboratory (Halmstad), as well as an Icelandic Ad41 isolate analyzed by us, were found to be D12 [22]. The low incidence of Ad40 during the period studied is consistent with what has been reported from the United Kingdom [40], Canada [12], Japan [33] and the Netherlands [14].…”

Comparative epidemiology of rotavirus, subgenus F (types 40 and 41) adenovirus, and astrovirus gastroenteritis in children

“…The only study comparing all 3 viruses amalgamated data from several centres without any certainty over the uniformity of surveillance methods used in each centre [Lew et al, 19901. Moreover, the epidemiology of the 2 subgenus F adenovirus serotypes (40 and 41) has been compared to only a limited extent [Willcocks et al, 1988;Shinozaki et al, 19911. We report a 5-year prospective analysis of rotavirus, subgenus F (type 40 and 41) adenovirus, and astrovirus gastroenteritis in hospitalised children.…”

Growth and characterisation of human faecal astrovirus in a continuous cell line

“…None of the samples induced cpe in HeLa or Vero cells in the absence of trypsin. In the presence of this enzyme the monolayers of these cells were destroyed re- half of the sample was processed for electron microscopy [19]. Subsequent passages were performed in exactly the same manner except that a 0.5 ml aliquot of the previous infected cell lysate was used as inoculum.…”