Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation

Kisiala, M.; Kowalska, Monika; Pastor, Michal; Korza, Henryk J.; Czapinska, H.; Bochtler, Matthias

doi:10.1093/nar/gkaa403

Cited by 5 publications

(3 citation statements)

References 70 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H. pylori has been derived from a variety of clinical specimens and identified using a multiplex polymerase chain reaction (Multiplex-PCR). Several virulence genes in H. pylori isolates have been reported, including Vacuolating cytotoxin A ( VacA ) 17 , cytotoxin-associated A ( cagA ) 17 , restriction endonuclease A ( IceA ) 18 , Outer inflammatory protein A ( OipA ) 19 , and blood-group antigen-binding adhesin ( BabA2 ) 19 . These pathogenicity genes may have a role in the progression of H. pylori .…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics

Piri-Gharaghie,

Ghajari,

Tolou-Shikhzadeh-Yazdi

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, we looked at the distribution of the pathogenicity cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antimicrobial resistance. Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 raw poultry specimens. Disk diffusion and Multiplex-PCR were used to investigate antimicrobial resistance and genotyping patterns, separately. H. pylori was found in 20 of 320 (6.25%) raw poultry samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas the fewest was found in Goose and Quebec (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were greatest in H. pylori isolates. The percentage of H. pylori isolates with a MAR value of more than 0.2 was 17/20 (85%). The most prevalent genotypes discovered were VacA s1a (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically discovered genotype patterns were s1am1a (45%), s2m1a (45%), and s2m2 (30%). BabA2, OipA + , and OipA− genotypes were found in 40%, 30%, and 30% of the population. In summary, the poultry flesh was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria implies a serious public health concern about raw poultry eating. In the future, researchers should look into H. pylori's resistance to multiple antibacterial drugs in Iran.

show abstract

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics

Piri-Gharaghie,

Ghajari,

Tolou-Shikhzadeh-Yazdi

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This needs to be avoided by replacing the type II restriction enzyme with type IIS restriction enzymes. 46,126 mRNA purification. IVT mRNAs are mixed with RNA polymerase and DNA templates after synthesis; thus, it is essential to purify IVT mRNA, including removing immunostimulatory contaminants, free ribonucleotides, short mRNA and DNA templates.…”

Section: Rna Chemical Formula Designmentioning

confidence: 99%

mRNA-based therapeutics: powerful and versatile tools to combat diseases

Qin

Tang

Chen

et al. 2022

Sig Transduct Target Ther

251

168

View full text Add to dashboard Cite

The therapeutic use of messenger RNA (mRNA) has fueled great hope to combat a wide range of incurable diseases. Recent rapid advances in biotechnology and molecular medicine have enabled the production of almost any functional protein/peptide in the human body by introducing mRNA as a vaccine or therapeutic agent. This represents a rising precision medicine field with great promise for preventing and treating many intractable or genetic diseases. In addition, in vitro transcribed mRNA has achieved programmed production, which is more effective, faster in design and production, as well as more flexible and cost-effective than conventional approaches that may offer. Based on these extraordinary advantages, mRNA vaccines have the characteristics of the swiftest response to large-scale outbreaks of infectious diseases, such as the currently devastating pandemic COVID-19. It has always been the scientists’ desire to improve the stability, immunogenicity, translation efficiency, and delivery system to achieve efficient and safe delivery of mRNA. Excitingly, these scientific dreams have gradually been realized with the rapid, amazing achievements of molecular biology, RNA technology, vaccinology, and nanotechnology. In this review, we comprehensively describe mRNA-based therapeutics, including their principles, manufacture, application, effects, and shortcomings. We also highlight the importance of mRNA optimization and delivery systems in successful mRNA therapeutics and discuss the key challenges and opportunities in developing these tools into powerful and versatile tools to combat many genetic, infectious, cancer, and other refractory diseases.

show abstract

“…H. pylori pathogenesis is linked to virulence genes only found in certain groups. Several virulence genes in H. pylori isolates have been reported, including vacuolating cytotoxin A (VacA) [23], cytotoxin-associated A (CagA) [23], restriction endonuclease A (IceA) [24], outer inflammatory protein A (OipA) [25], and blood-group antigen-binding adhesin (BabA2) [25]. Among the most efficient ways for studying relationships between H. pylori strains from diverse samples is genotyping using the virulence genes (cagA, vacA, babA2, oipA, and iceA) [15].…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori Strains Isolated from Raw Poultry Meat in the Shahrekord Region, Iran: Frequency and Molecular Characteristics

Asadi

Rahimi

Shakerian

2023

Genes

View full text Add to dashboard Cite

Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (chicken, turkey, quail, goose, and ostrich) is consumed as a regular protein source by many people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, the distribution of the virulence genes cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antibacterial resistance, was investigated. A Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 samples of raw poultry meat. Disk diffusion and multiplex-PCR were used to investigate both antimicrobial resistance and genotyping patterns. H. pylori was found in 20 of 320 (6.25 %) raw chicken meat samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas no isolate was recovered from goose or quail raw meat (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were the most commonly detected in the tested H. pylori isolates. The percentage of H. pylori isolates with a multiple antibiotic resistance (MAR) index value of more than 0.2 was 17/20 (85%). The most prevalent genotypes detected were VacA (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically detected genotype patterns were s1am1a (45 %), s2m1a (45 %), and s2 m2 (30 %). babA2, oipA+, and oipA- genotypes were found in 40%, 30%, and 30% of the population, respectively. In summary, fresh poultry meat was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria raises a serious public health concern regarding the consumption of raw poultry. Future research should evaluate antimicrobial resistance among H. pylori isolates in Iran.

show abstract

Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation

Cited by 5 publications

References 70 publications

RETRACTED ARTICLE: Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics

RETRACTED ARTICLE: Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics

mRNA-based therapeutics: powerful and versatile tools to combat diseases

Helicobacter pylori Strains Isolated from Raw Poultry Meat in the Shahrekord Region, Iran: Frequency and Molecular Characteristics

Contact Info

Product

Resources

About